Publicação
In vivo toxicity evaluation of Carbon nanoparticles
| Resumo: | In recent years, the interest in nanotechnology has increased dramatically due to its wide range applications. However, studies have emerged that raise awareness about the effects that the use of nanoparticles (NPs) can have on both the population and the environment, making it relevant to understand the toxic effect that these nanoparticles can have on animal models. Carbon NPs (NPCs), in particular carbon dots (CDots), have attracted the attention of specialists due to their excellent biocompatibility. The objective of this study was to evaluate the toxicity of NPCs (CDots) in vivo, and the reactive oxygen species (ROS) produced by NPCs when exposed to ultraviolet radiation. In this sense, Drosophila melanogaster was used as an animal model. In order to evaluate the possible toxic effect of the NPCs, they were placed in the Drosophila food, at different concentrations (0.0, 0.4 and 0.8 mM). The following parameters where evaluated: mortality, the number of eggs, the number of pupae, the locomotion in each section, the average distance travelled by the larvae and the number of descendant flies. These parameters were evaluated in three assays: A1 – NPCs not exposed to UV; A2 – Use of females and males from the F1 generation 0.4 mM of NPCs not exposed to UV and A3 – NPCs exposed to UV (400 nm) for 30 min. It was found that NPCs did not have a statistically significant toxic effect on Parental (P1) survival and average distance travelled by the F1 generation. On the other hand, a statistically harmful effect of NPCs was identified on the number of eggs produced, on locomotion and on the number of flies that reached the adult state in tests A1 and A3, and in A3 exposure to ultraviolet radiation did not have an increased statistically significant effect. Regarding the A2 test, it was found that there is a negative transgenerational effect (after exposure of P1 and F1 to NPCs) on the number of pupae of the F2 generation. However, as seen previously for the A1 test, the decrease in the number of adult flies was only verified due to the lower number of formed pupae, not due to a direct effect of the NPCs on hatching. Thus, it appears that the exposure of flies to ROS produced by NPCs did not increase their toxicity, since the results obtained for the A1 and A3 tests where very similar. Therefore, the additional exposure to these stress-inducing elements did not change any of the variables under study, not ruling out the hypothesis that they may have had an influence on something that was not the subject of this study. |
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| Autores principais: | Sousa, Luís Carlos Faria de |
| Assunto: | Nanoparticles Carbon dots |
| Ano: | 2023 |
| País: | Portugal |
| Tipo de documento: | dissertação de mestrado |
| Tipo de acesso: | acesso aberto |
| Instituição associada: | Universidade de Trás-os-Montes e Alto Douro |
| Idioma: | inglês |
| Origem: | Repositório da UTAD |
| Resumo: | In recent years, the interest in nanotechnology has increased dramatically due to its wide range applications. However, studies have emerged that raise awareness about the effects that the use of nanoparticles (NPs) can have on both the population and the environment, making it relevant to understand the toxic effect that these nanoparticles can have on animal models. Carbon NPs (NPCs), in particular carbon dots (CDots), have attracted the attention of specialists due to their excellent biocompatibility. The objective of this study was to evaluate the toxicity of NPCs (CDots) in vivo, and the reactive oxygen species (ROS) produced by NPCs when exposed to ultraviolet radiation. In this sense, Drosophila melanogaster was used as an animal model. In order to evaluate the possible toxic effect of the NPCs, they were placed in the Drosophila food, at different concentrations (0.0, 0.4 and 0.8 mM). The following parameters where evaluated: mortality, the number of eggs, the number of pupae, the locomotion in each section, the average distance travelled by the larvae and the number of descendant flies. These parameters were evaluated in three assays: A1 – NPCs not exposed to UV; A2 – Use of females and males from the F1 generation 0.4 mM of NPCs not exposed to UV and A3 – NPCs exposed to UV (400 nm) for 30 min. It was found that NPCs did not have a statistically significant toxic effect on Parental (P1) survival and average distance travelled by the F1 generation. On the other hand, a statistically harmful effect of NPCs was identified on the number of eggs produced, on locomotion and on the number of flies that reached the adult state in tests A1 and A3, and in A3 exposure to ultraviolet radiation did not have an increased statistically significant effect. Regarding the A2 test, it was found that there is a negative transgenerational effect (after exposure of P1 and F1 to NPCs) on the number of pupae of the F2 generation. However, as seen previously for the A1 test, the decrease in the number of adult flies was only verified due to the lower number of formed pupae, not due to a direct effect of the NPCs on hatching. Thus, it appears that the exposure of flies to ROS produced by NPCs did not increase their toxicity, since the results obtained for the A1 and A3 tests where very similar. Therefore, the additional exposure to these stress-inducing elements did not change any of the variables under study, not ruling out the hypothesis that they may have had an influence on something that was not the subject of this study. |
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