Publicação
Avaliação de óleos essenciais e extratos de Lavandula SPP. no controlo de microrganismos
| Resumo: | The Portuguese flora is very rich in aromatic and medicinal plants, among which are Lavandula stoechas subsp. luisieri and L. pedunculata. The present study was aimed to evaluate the potential antimicrobial activity of five plant extracts and essential oils (EO) obtained from these species, with respect to human and plant pathogens. The EO were extracted from dried material by hydrodistillation and the extracts by a solvent system of increasing polarity. To evaluate the activity of samples against human pathogens the microdilution method was used and determined the values of minimum inhibitory concentration (MIC) at different concentrations: 7.5, 15.5, 31, 62.5, 125, 250 and 500 μg/mL. Results were considered positives for MIC values < 100 μg/mL. Lavandula species showed different antimicrobial properties, attributed to differences in the chemical composition of the respective EO and extracts. For phytopathogenic bacteria a similar procedure was followed. Regarding the fungi, the activity of EO and extracts was evaluated by the disc diffusion method on solid medium inoculated with spore suspensions or mycelial discs at sample concentration of 1 mg/mL and 2 mg/mL, respectively. None of the plant pathogens was inhibited. This study tends to confirm the use of EO and plant extracts to control different types of microbial pathogens |
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| Autores principais: | Jorge, Rita Eduardo |
| Assunto: | Lavandula stoechas subsp. luisieri Lavandula pedunculata essential oils extracts antimicrobial activity |
| Ano: | 2014 |
| País: | Portugal |
| Tipo de documento: | dissertação de mestrado |
| Tipo de acesso: | acesso aberto |
| Instituição associada: | Universidade de Lisboa |
| Idioma: | português |
| Origem: | Repositório da Universidade de Lisboa |
| Resumo: | The Portuguese flora is very rich in aromatic and medicinal plants, among which are Lavandula stoechas subsp. luisieri and L. pedunculata. The present study was aimed to evaluate the potential antimicrobial activity of five plant extracts and essential oils (EO) obtained from these species, with respect to human and plant pathogens. The EO were extracted from dried material by hydrodistillation and the extracts by a solvent system of increasing polarity. To evaluate the activity of samples against human pathogens the microdilution method was used and determined the values of minimum inhibitory concentration (MIC) at different concentrations: 7.5, 15.5, 31, 62.5, 125, 250 and 500 μg/mL. Results were considered positives for MIC values < 100 μg/mL. Lavandula species showed different antimicrobial properties, attributed to differences in the chemical composition of the respective EO and extracts. For phytopathogenic bacteria a similar procedure was followed. Regarding the fungi, the activity of EO and extracts was evaluated by the disc diffusion method on solid medium inoculated with spore suspensions or mycelial discs at sample concentration of 1 mg/mL and 2 mg/mL, respectively. None of the plant pathogens was inhibited. This study tends to confirm the use of EO and plant extracts to control different types of microbial pathogens |
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