Publicação
Skin prick tests : preliminary evaluation of this technique for the diagnosis of canine atopic dermatitis sensitization
| Resumo: | Canine atopic dermatitis (CAD) is a multifactorial disease involving a type I hypersensitivity to allergens, cutaneous barrier defects, microbial infections and other flare factors. It is “an inflammatory and pruritic skin disease, with genetic predisposition and characteristic clinical features associated with IgE antibodies most commonly directed against environmental allergens”. The clinical signs of CAD frequently manifest from 6 months to 3 years of age. Some breeds, such as Bull Terrier, Cocker Spaniel, English Bulldog, German Shepherd Dog, Golden Retriever, Labrador Retriever, Pug, Shar Pei, West Highland White Terrier, Yorkshire Terrier, among others, seem to be at a higher risk of developing the disease. The lesions are typically distributed in the periocular skin, muzzle, ears, interdigital area, flexural joints of the extremities, axillae, abdomen, groin and perineum. The first clinical manifestation is usually pruritus with erythema, followed by secondary lesions, such as excoriations, self-induced alopecia, dry hair, hyperpigmentation, scaling and lichenification, which reflect chronic pruritus and inflammation, concurrent secondary infections and bacterial overgrowth. The diagnosis of the disease is based on the anamnesis, clinical signs, dermatological examination, exclusion of other pruritic skin diseases and allergy testing, with in vivo techniques, such as Intradermal Tests (IDT) or Skin Prick Tests (SPT), and in vitro methods, such as, allergen-specific IgE measurements. CAD has no cure, however it is possible to be controlled with multifactorial treatment, which may include allergen avoidance measures, allergen-specific immunotherapy (ASIT), improvement of the skin barrier function and anti-inflammatory therapy. The objective of this study was to determine if SPT technique was doable in dogs. Also, it was important to determine whether or not the standardized concentrations of the allergens used for human patients would cause irritant false positive skin reactions in healthy non-atopic dogs. Therefore, 22 healthy non-atopic dogs were tested with 15 aqueous allergens commercially available for use in human medicine. The results showed that all dogs had negative skin reactions to the concentrations available for each allergen tested. It was possible to conclude that the SPT were not only doable but they showed many advantages versus IDT, such as simplicity, rapidity, less discomfort, less irritation and safety, and also, that the concentrations used did not cause irritant false positive skin reactions in healthy dogs. |
|---|---|
| Autores principais: | Rocha, Maria Inês Lopes |
| Assunto: | Canine atopic dermatitis skin prick tests allergens Dermatite atópica canina testes cutâneos Prick alergénios |
| Ano: | 2012 |
| País: | Portugal |
| Tipo de documento: | dissertação de mestrado |
| Tipo de acesso: | acesso aberto |
| Instituição associada: | Universidade de Lisboa |
| Idioma: | inglês |
| Origem: | Repositório da Universidade de Lisboa |
| Resumo: | Canine atopic dermatitis (CAD) is a multifactorial disease involving a type I hypersensitivity to allergens, cutaneous barrier defects, microbial infections and other flare factors. It is “an inflammatory and pruritic skin disease, with genetic predisposition and characteristic clinical features associated with IgE antibodies most commonly directed against environmental allergens”. The clinical signs of CAD frequently manifest from 6 months to 3 years of age. Some breeds, such as Bull Terrier, Cocker Spaniel, English Bulldog, German Shepherd Dog, Golden Retriever, Labrador Retriever, Pug, Shar Pei, West Highland White Terrier, Yorkshire Terrier, among others, seem to be at a higher risk of developing the disease. The lesions are typically distributed in the periocular skin, muzzle, ears, interdigital area, flexural joints of the extremities, axillae, abdomen, groin and perineum. The first clinical manifestation is usually pruritus with erythema, followed by secondary lesions, such as excoriations, self-induced alopecia, dry hair, hyperpigmentation, scaling and lichenification, which reflect chronic pruritus and inflammation, concurrent secondary infections and bacterial overgrowth. The diagnosis of the disease is based on the anamnesis, clinical signs, dermatological examination, exclusion of other pruritic skin diseases and allergy testing, with in vivo techniques, such as Intradermal Tests (IDT) or Skin Prick Tests (SPT), and in vitro methods, such as, allergen-specific IgE measurements. CAD has no cure, however it is possible to be controlled with multifactorial treatment, which may include allergen avoidance measures, allergen-specific immunotherapy (ASIT), improvement of the skin barrier function and anti-inflammatory therapy. The objective of this study was to determine if SPT technique was doable in dogs. Also, it was important to determine whether or not the standardized concentrations of the allergens used for human patients would cause irritant false positive skin reactions in healthy non-atopic dogs. Therefore, 22 healthy non-atopic dogs were tested with 15 aqueous allergens commercially available for use in human medicine. The results showed that all dogs had negative skin reactions to the concentrations available for each allergen tested. It was possible to conclude that the SPT were not only doable but they showed many advantages versus IDT, such as simplicity, rapidity, less discomfort, less irritation and safety, and also, that the concentrations used did not cause irritant false positive skin reactions in healthy dogs. |
|---|
Atividades financiadas
Não foram encontrados projetos financiados.