Publicação
Coaxial electrospun PCL/Gelatin-MA fibers as scaffolds for vascular tissue engineering
| Resumo: | Coaxial electrospinning is a technique that allows the production of nanofibers with a core–shell structure. Such fibers present several advantages as materials for the preparation of scaffolds, namely due to the possibility of combining a core with the desired mechanical properties with a shell prepared from biocompatible materials that will establish proper interactions with the host. Herein, core-shell fibrous meshes, composed of a polycaprolactone (PCL) core and a functionalized gelatin shell, were prepared by coaxial electrospinning and then photocrosslinked under UV light aiming to be used in vascular tissue regeneration. The suitability of the meshes for the pretended biomedical application was evaluated by assessing their chemical/physical properties as well as their haemo and biocompatibility in vitro. The obtained results revealed that meshes’ shell prepared with a higher content of gelatin showed fibers with diameters presenting a unimodal distribution and a mean value of 600 nm. Moreover, those fibers with higher content of gelatin also displayed lower water contact angles, and therefore higher hydrophilicities. Such features are crucial for the good biologic performance displayed by these meshes, when in contact with blood and with Normal Human Dermal Fibroblasts cells. |
|---|---|
| Autores principais: | Coimbra, Patrícia |
| Outros Autores: | Santos, Patrícia; Alves, Patrícia; Miguel, Sónia P.; Carvalho, Marco António Paulo de; Sá, Kevin; Correia, Ilídio Joaquim Sobreira; Ferreira, Paula |
| Assunto: | Coaxial electrospinning Photocrosslinking Scaffolds Tissue engineering Biocompatibility |
| Ano: | 2017 |
| País: | Portugal |
| Tipo de documento: | artigo |
| Tipo de acesso: | acesso restrito |
| Instituição associada: | Universidade da Beira Interior |
| Idioma: | inglês |
| Origem: | uBibliorum |
| _version_ | 1868420777349480448 |
|---|---|
| author | Coimbra, Patrícia |
| author2 | Santos, Patrícia Alves, Patrícia Miguel, Sónia P. Carvalho, Marco António Paulo de Sá, Kevin Correia, Ilídio Joaquim Sobreira Ferreira, Paula |
| author2_role | author author author author author author author |
| author_facet | Coimbra, Patrícia Santos, Patrícia Alves, Patrícia Miguel, Sónia P. Carvalho, Marco António Paulo de Sá, Kevin Correia, Ilídio Joaquim Sobreira Ferreira, Paula |
| author_role | author |
| contributor_name_str_mv | uBibliorum |
| country_str | PT |
| creators_json_txt | [{\"Person.name\":\"Coimbra, Patrícia\"},{\"Person.name\":\"Santos, Patrícia\"},{\"Person.name\":\"Alves, Patrícia\",\"Person.identifier.orcid\":\"0000-0002-8943-8329\"},{\"Person.name\":\"Miguel, Sónia P.\",\"Person.identifier.orcid\":\"0000-0001-7274-0399\"},{\"Person.name\":\"Carvalho, Marco António Paulo de\"},{\"Person.name\":\"Sá, Kevin\",\"Person.identifier.orcid\":\"0000-0002-7153-3272\"},{\"Person.name\":\"Correia, Ilídio Joaquim Sobreira\",\"Person.identifier.orcid\":\"0000-0003-1613-9675\"},{\"Person.name\":\"Ferreira, Paula\",\"Person.identifier.orcid\":\"0000-0003-3393-4427\"}] |
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| datacite.creators.creator.creatorName.fl_str_mv | Coimbra, Patrícia Santos, Patrícia Alves, Patrícia Miguel, Sónia P. Carvalho, Marco António Paulo de Sá, Kevin Correia, Ilídio Joaquim Sobreira Ferreira, Paula |
| datacite.date.Accepted.fl_str_mv | 2017-07-26T00:00:00Z |
| datacite.date.available.fl_str_mv | 2018-03-22T10:13:07Z |
| datacite.date.embargoed.fl_str_mv | 2018-03-22T10:13:07Z |
| datacite.rights.fl_str_mv | http://purl.org/coar/access_right/c_16ec |
| datacite.subjects.subject.fl_str_mv | Coaxial electrospinning Photocrosslinking Scaffolds Tissue engineering Biocompatibility |
| datacite.titles.title.fl_str_mv | Coaxial electrospun PCL/Gelatin-MA fibers as scaffolds for vascular tissue engineering |
| dc.contributor.none.fl_str_mv | uBibliorum |
| dc.creator.none.fl_str_mv | Coimbra, Patrícia Santos, Patrícia Alves, Patrícia Miguel, Sónia P. Carvalho, Marco António Paulo de Sá, Kevin Correia, Ilídio Joaquim Sobreira Ferreira, Paula |
| dc.date.Accepted.fl_str_mv | 2017-07-26T00:00:00Z |
| dc.date.available.fl_str_mv | 2018-03-22T10:13:07Z |
| dc.date.embargoed.fl_str_mv | 2018-03-22T10:13:07Z |
| dc.format.none.fl_str_mv | application/pdf |
| dc.identifier.none.fl_str_mv | http://hdl.handle.net/10400.6/4706 |
| dc.language.none.fl_str_mv | eng |
| dc.publisher.none.fl_str_mv | Elsevier |
| dc.rights.none.fl_str_mv | http://purl.org/coar/access_right/c_16ec |
| dc.subject.none.fl_str_mv | Coaxial electrospinning Photocrosslinking Scaffolds Tissue engineering Biocompatibility |
| dc.title.fl_str_mv | Coaxial electrospun PCL/Gelatin-MA fibers as scaffolds for vascular tissue engineering |
| dc.type.none.fl_str_mv | http://purl.org/coar/resource_type/c_6501 |
| description | Coaxial electrospinning is a technique that allows the production of nanofibers with a core–shell structure. Such fibers present several advantages as materials for the preparation of scaffolds, namely due to the possibility of combining a core with the desired mechanical properties with a shell prepared from biocompatible materials that will establish proper interactions with the host. Herein, core-shell fibrous meshes, composed of a polycaprolactone (PCL) core and a functionalized gelatin shell, were prepared by coaxial electrospinning and then photocrosslinked under UV light aiming to be used in vascular tissue regeneration. The suitability of the meshes for the pretended biomedical application was evaluated by assessing their chemical/physical properties as well as their haemo and biocompatibility in vitro. The obtained results revealed that meshes’ shell prepared with a higher content of gelatin showed fibers with diameters presenting a unimodal distribution and a mean value of 600 nm. Moreover, those fibers with higher content of gelatin also displayed lower water contact angles, and therefore higher hydrophilicities. Such features are crucial for the good biologic performance displayed by these meshes, when in contact with blood and with Normal Human Dermal Fibroblasts cells. |
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| identifier.url.fl_str_mv | http://hdl.handle.net/10400.6/4706 |
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| person_str_mv | Coimbra, Patrícia Coimbra, Patrícia https://www.ciencia-id.pt/111E-0CE2-0C3E 111E-0CE2-0C3E Santos, Patrícia Alves, Patrícia Alves, Patrícia https://www.ciencia-id.pt/341B-551C-19FD 341B-551C-19FD http://orcid.org/0000-0002-8943-8329 0000-0002-8943-8329 Miguel, Sónia P. Miguel, Sónia P. https://www.ciencia-id.pt/1A13-ABC0-C32C 1A13-ABC0-C32C http://orcid.org/0000-0001-7274-0399 0000-0001-7274-0399 Carvalho, Marco António Paulo de Sá, Kevin Sá, Kevin http://orcid.org/0000-0002-7153-3272 0000-0002-7153-3272 Correia, Ilídio Joaquim Sobreira Correia, Ilídio Joaquim Sobreira https://www.ciencia-id.pt/F610-7373-DC81 F610-7373-DC81 http://orcid.org/0000-0003-1613-9675 0000-0003-1613-9675 Ferreira, Paula Ferreira, Paula https://www.ciencia-id.pt/A119-FFB8-0899 A119-FFB8-0899 http://orcid.org/0000-0003-3393-4427 0000-0003-3393-4427 |
| publishDate | 2017 |
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| spelling | engElsevierpt_PTCoaxial electrospinning is a technique that allows the production of nanofibers with a core–shell structure. Such fibers present several advantages as materials for the preparation of scaffolds, namely due to the possibility of combining a core with the desired mechanical properties with a shell prepared from biocompatible materials that will establish proper interactions with the host. Herein, core-shell fibrous meshes, composed of a polycaprolactone (PCL) core and a functionalized gelatin shell, were prepared by coaxial electrospinning and then photocrosslinked under UV light aiming to be used in vascular tissue regeneration. The suitability of the meshes for the pretended biomedical application was evaluated by assessing their chemical/physical properties as well as their haemo and biocompatibility in vitro. The obtained results revealed that meshes’ shell prepared with a higher content of gelatin showed fibers with diameters presenting a unimodal distribution and a mean value of 600 nm. Moreover, those fibers with higher content of gelatin also displayed lower water contact angles, and therefore higher hydrophilicities. Such features are crucial for the good biologic performance displayed by these meshes, when in contact with blood and with Normal Human Dermal Fibroblasts cells.application/pdfpt_PTCoaxial electrospun PCL/Gelatin-MA fibers as scaffolds for vascular tissue engineeringPersonalCoimbra, PatríciaDSpacehttp://dspace.org/items/95a46930-ac14-449d-8ddd-db23927062b0DSpacehttp://dspace.org/items/95a46930-ac14-449d-8ddd-db23927062b0Almeida CoimbraPatrícia ManuelaCiência IDhttps://www.ciencia-id.pt111E-0CE2-0C3ESantos, PatríciaPersonalAlves, PatríciaDSpacehttp://dspace.org/items/64a3aef7-8d91-4327-8407-294909eed51aDSpacehttp://dspace.org/items/64a3aef7-8d91-4327-8407-294909eed51aAlvesPatríciaCiência IDhttps://www.ciencia-id.pt341B-551C-19FDORCIDhttp://orcid.org0000-0002-8943-8329Researcher IDhttps://www.researcherid.comI-1004-2013Scopus Author IDhttps://www.scopus.com12762379800PersonalMiguel, Sónia P.DSpacehttp://dspace.org/items/3956033a-4abf-439b-a4fd-25c5bd9d8e7aDSpacehttp://dspace.org/items/3956033a-4abf-439b-a4fd-25c5bd9d8e7aPereira MiguelSónia AlexandraCiência IDhttps://www.ciencia-id.pt1A13-ABC0-C32CORCIDhttp://orcid.org0000-0001-7274-0399Scopus Author IDhttps://www.scopus.com55638166400Carvalho, Marco António Paulo dePersonalSá, KevinDSpacehttp://dspace.org/items/29c91d07-360c-4414-89c5-347c6a69de2fDSpacehttp://dspace.org/items/29c91d07-360c-4414-89c5-347c6a69de2fSáKevinORCIDhttp://orcid.org0000-0002-7153-3272PersonalCorreia, Ilídio Joaquim SobreiraDSpacehttp://dspace.org/items/f2c3e779-34a2-4309-950a-80687664c6adDSpacehttp://dspace.org/items/f2c3e779-34a2-4309-950a-80687664c6adCorreiaIlídio Joaquim SobreiraCiência IDhttps://www.ciencia-id.ptF610-7373-DC81ORCIDhttp://orcid.org0000-0003-1613-9675Scopus Author IDhttps://www.scopus.com7003557499PersonalFerreira, PaulaDSpacehttp://dspace.org/items/a7e98e57-6817-4cd2-95c5-42ebadda09c5DSpacehttp://dspace.org/items/a7e98e57-6817-4cd2-95c5-42ebadda09c5CalvinhoPaula Cristina Nunes FerreiraCiência IDhttps://www.ciencia-id.ptA119-FFB8-0899ORCIDhttp://orcid.org0000-0003-3393-4427Scopus Author IDhttps://www.scopus.com55321888200HostingInstitutionOrganizationaluBibliorume-mailmailto:noreplyuBibliorum@ubi.ptnoreplyuBibliorum@ubi.ptDOIIsPartOf10.1016/j.colsurfb.2017.07.0652018-03-22T10:13:07Z2017-07-262017-07-26T00:00:00ZHandlehttp://hdl.handle.net/10400.6/4706http://purl.org/coar/access_right/c_16ecrestricted accessCoaxial electrospinningPhotocrosslinkingScaffoldsTissue engineeringBiocompatibility4803007 bytesFundação para a Ciência e a TecnologiaHealth Sciences Research Centre [UID/Multi/00709/2019]info:eu-repo/grantAgreement/FCT/6817 - DCRRNI ID/UID%2FMulti%2F00709%2F2019/PTUID/Multi/00709/20196817 - DCRRNI IDCrossref Funder IDhttp://doi.org/10.13039/501100001871Fundação para a Ciência e a TecnologiaBolsa de Pós-Doutoramento FCT: Utilização de enxertos de osso alógeno e materiais derivados do seu processamento na preparação de sistemas de libertação controlada e localizada de antibióticos para aplicações em cirurgia ortopédica [SFRH/BPD/73367/2010]info:eu-repo/grantAgreement/FCT/FARH/SFRH%2FBPD%2F73367%2F2010/PTSFRH/BPD/73367/2010FARHBolsa de Pós-DoutoramentoCrossref Funder IDhttp://doi.org/10.13039/501100001871Fundação para a Ciência e a TecnologiaDevelopment of New Stimuli-Responsive Polymer-Liposome Complexes by Living Radical Polymerization and “Click Chemistry” [SFRH/BPD/69410/2010]info:eu-repo/grantAgreement/FCT//SFRH%2FBPD%2F69410%2F2010/PTSFRH/BPD/69410/2010Crossref Funder IDhttp://doi.org/10.13039/501100001871Fundação para a Ciência e a TecnologiaBolsa de Pós-Doutoramento FCT: Pharmacologically Active Bioadhesive Designed for Wound Closure [SFRH/BPD/86338/2012]info:eu-repo/grantAgreement/FCT/OE/SFRH%2FBPD%2F86338%2F2012/PTSFRH/BPD/86338/2012OEBolsa de Pós-DoutoradoCrossref Funder IDhttp://doi.org/10.13039/501100001871Fundação para a Ciência e a TecnologiaBolsa de Doutoramento FCT: Development of a bioactive nanofibrous membrane loaded with bioactive molecules for wound healing [SFRH/BD/109563/2015]info:eu-repo/grantAgreement/FCT/POR_CENTRO/SFRH%2FBD%2F109563%2F2015/PTSFRH/BD/109563/2015POR_CENTROBolsa de DoutoramentoCrossref Funder IDhttp://doi.org/10.13039/501100001871literaturehttp://purl.org/coar/resource_type/c_6501journal articlehttp://purl.org/coar/access_right/c_16ecapplication/pdffulltexthttps://ubibliorum.ubi.pt/bitstreams/3eedc379-3687-4874-9b0e-198000914237/downloadColloids and Surfaces B: Biointerfaces159715 |
| spellingShingle | Coaxial electrospun PCL/Gelatin-MA fibers as scaffolds for vascular tissue engineering Coimbra, Patrícia Coaxial electrospinning Photocrosslinking Scaffolds Tissue engineering Biocompatibility |
| status | SINGLETON |
| subject.fl_str_mv | Coaxial electrospinning Photocrosslinking Scaffolds Tissue engineering Biocompatibility |
| title | Coaxial electrospun PCL/Gelatin-MA fibers as scaffolds for vascular tissue engineering |
| title_full | Coaxial electrospun PCL/Gelatin-MA fibers as scaffolds for vascular tissue engineering |
| title_fullStr | Coaxial electrospun PCL/Gelatin-MA fibers as scaffolds for vascular tissue engineering |
| title_full_unstemmed | Coaxial electrospun PCL/Gelatin-MA fibers as scaffolds for vascular tissue engineering |
| title_short | Coaxial electrospun PCL/Gelatin-MA fibers as scaffolds for vascular tissue engineering |
| title_sort | Coaxial electrospun PCL/Gelatin-MA fibers as scaffolds for vascular tissue engineering |
| topic | Coaxial electrospinning Photocrosslinking Scaffolds Tissue engineering Biocompatibility |
| topic_facet | Coaxial electrospinning Photocrosslinking Scaffolds Tissue engineering Biocompatibility |
| url | http://hdl.handle.net/10400.6/4706 |
| visible | 1 |