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Molecular cloning and sequence of gilthead sea bream (Sparus aurata) alpha-skeletal actin: tissue and developmental expression

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Detalhes bibliográficos
Resumo:In the present study, the Sparus aurata -skeletal actin was cloned from a mixed larvae complementary DNA library. The clone isolated was 1523 bp long with an open reading frame of 1134 bp coding for a 377-amino acid protein. The deduced amino acid sequence of sea bream -actin is identical to Fugu -actin-1. The expression of -actin was initiated at the onset of segmentation. In adult fish, -actin is expressed predominantly in white and red muscle.
Autores principais:Moutou, K. A.
Outros Autores:Socorro, Sílvia; Power, Deborah; Mamuris, Z.; Canario, Adelino V. M.
Assunto:Teleost Skeletal muscle Actin cDNA Gilthead sea bream ŽSparus aurata
Ano:2001
País:Portugal
Tipo de documento:artigo
Tipo de acesso:acesso restrito
Instituição associada:Universidade do Algarve
Idioma:inglês
Origem:Sapientia - Universidade do Algarve
Descrição
Resumo:In the present study, the Sparus aurata -skeletal actin was cloned from a mixed larvae complementary DNA library. The clone isolated was 1523 bp long with an open reading frame of 1134 bp coding for a 377-amino acid protein. The deduced amino acid sequence of sea bream -actin is identical to Fugu -actin-1. The expression of -actin was initiated at the onset of segmentation. In adult fish, -actin is expressed predominantly in white and red muscle.

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