Publication

Cloning and expression of an elongation factor-1α in sea bream ( Sparus aurata ) larvae and adult tissue

Bibliographic Details
Summary:	A clone encoding the polypeptide elongation factor EF-1a was isolated from a complementary DNA library prepared from sea bream (Spartus aurata) larvae 1 to 10 days after hatching. The deduced amino acid sequence is between 82% and 95% similar to EF-1a in other animal species. EF-1a messenger RNA is present at low abundance in sea bream embryos prior to gastrulation, but at around 15 hours postfertilization, there is a 10-fold increase in transcript levels. This increase presumably reflects midblastula transition in this species. In adult sea bream, EF-1a appeared to have a relatively uniform distribution across all the tissues analyzed.
Main Authors:	Nowell, M. A.
Other Authors:	Power, Deborah; Guerreiro, P. M.; Llewellyn, Lynda; Ramsurn, Vimi P.; Wigham, Trevor; Sweeney, Glen E.
Subject:	EF-1a cDNA Expression Sea beam (sparus aurata) Teleost Larvae Adult tissue
Year:	2000
Country:	Portugal
Document type:	article
Access type:	restricted access
Associated institution:	Universidade do Algarve
Language:	English
Origin:	Sapientia - Universidade do Algarve

Description
Summary:	A clone encoding the polypeptide elongation factor EF-1a was isolated from a complementary DNA library prepared from sea bream (Spartus aurata) larvae 1 to 10 days after hatching. The deduced amino acid sequence is between 82% and 95% similar to EF-1a in other animal species. EF-1a messenger RNA is present at low abundance in sea bream embryos prior to gastrulation, but at around 15 hours postfertilization, there is a 10-fold increase in transcript levels. This increase presumably reflects midblastula transition in this species. In adult sea bream, EF-1a appeared to have a relatively uniform distribution across all the tissues analyzed.

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