Publicação
Innovative contributions on calibration methodologies towards reliable microflow measurements
| Resumo: | Flow measurement is critical in healthcare, chemistry and pharmaceutics, to mention a few. In fact, there are several applications in the microflow and nanoflow range, such as scaled-down process technology, drug development, and special health-care applications, as organ-on-a- chip technology. Nevertheless, the majority of the instruments used for the specified applica- tions are not sufficiently studied regarding their flow accuracy and traceability. Hence these fluid applications at the micro and nanoscale still lack well defined calibration methodologies for the devices working at the mentioned flow range with adequate uncertainty values. The work presented in this thesis focuses on the development and improvement of in- novative applications of calibration methodologies for microflow measuring instruments. The gravimetric method already implemented at IPQ from 120 L/h to 2000 mL/h was used and improved for low flow rates down to 10 L/h. Additionally, other 4 methods were developed to enable the calibration of micro/nano flows in a non-intrusive way. They are interferometry, pending drop, front track and comparison method (where a calibrated flow generator is used as the reference standard). The methodology that is best suited for each specific instrument and each measurement range, with the lowest uncertainty, was successfully identified along with the relevant influence factors in microflow measurements. A specific objective of this work was to increase the measuring range of IPQ-LVC down to 5 nL/min (0.3 L/h) with a 3% target uncertainty (k=2). This objective was possible to achieve and even surpass with the use of the interferometric method, where measurements were per- formed down to 1.6 nL/min (0.1 L/h) with 2 % uncertainty(k=2). This method was internally validated by comparison with the gravimetric method and is now in the process of external validation by EURAMET project 1508. |
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| Autores principais: | Batista, Elsa Maria Isqueiro |
| Assunto: | Calibration microflow gravimetry interferometry drop front track principle |
| Ano: | 2022 |
| País: | Portugal |
| Tipo de documento: | tese de doutoramento |
| Tipo de acesso: | acesso aberto |
| Instituição associada: | Universidade Nova de Lisboa |
| Idioma: | inglês |
| Origem: | Repositório Institucional da UNL |
| Resumo: | Flow measurement is critical in healthcare, chemistry and pharmaceutics, to mention a few. In fact, there are several applications in the microflow and nanoflow range, such as scaled-down process technology, drug development, and special health-care applications, as organ-on-a- chip technology. Nevertheless, the majority of the instruments used for the specified applica- tions are not sufficiently studied regarding their flow accuracy and traceability. Hence these fluid applications at the micro and nanoscale still lack well defined calibration methodologies for the devices working at the mentioned flow range with adequate uncertainty values. The work presented in this thesis focuses on the development and improvement of in- novative applications of calibration methodologies for microflow measuring instruments. The gravimetric method already implemented at IPQ from 120 L/h to 2000 mL/h was used and improved for low flow rates down to 10 L/h. Additionally, other 4 methods were developed to enable the calibration of micro/nano flows in a non-intrusive way. They are interferometry, pending drop, front track and comparison method (where a calibrated flow generator is used as the reference standard). The methodology that is best suited for each specific instrument and each measurement range, with the lowest uncertainty, was successfully identified along with the relevant influence factors in microflow measurements. A specific objective of this work was to increase the measuring range of IPQ-LVC down to 5 nL/min (0.3 L/h) with a 3% target uncertainty (k=2). This objective was possible to achieve and even surpass with the use of the interferometric method, where measurements were per- formed down to 1.6 nL/min (0.1 L/h) with 2 % uncertainty(k=2). This method was internally validated by comparison with the gravimetric method and is now in the process of external validation by EURAMET project 1508. |
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