Publicação
Design of electrospun mats from natural polymers for skin regeneration
| Resumo: | Chronic wounds and burn injuries significantly impact global health, highlighting the limita- tions of current treatments that often fail to restore full skin functionality and sensitivity. This work presents the development of a dermal skin substitute composed of natural polymers—chitosan, fish gel- atin, and hyaluronic acid—designed to enhance skin regeneration and reinnervation. Electrospun scaffolds were fabricated using various combinations of these polymers and char- acterized for porosity (~48-84%), viscosity (~203-4150mPa·s), chemical composition using Fourier- transform infrared spectroscopy (FTIR), conductivity (~295-1130μS/cm), and morphology via scanning electron microscopy (SEM), which confirmed defect-free fibres with diameters of (~0.2-1.1μm). With a fibrous morphology mimicking the skin's native extracellular matrix, the scaffolds demonstrated ten- sile properties (~6-29 kPa) comparable to those of human skin. Further characterization included deg- radation rate (~0.2% over 64 days), swelling, and vapor transmission rate (~3700 g/m²·day). Biocompatibility was assessed using human fibroblasts (HFFF2) and neuroblastoma cells (SH- SY5Y). Cytotoxicity assays following ISO 10993-5 standards indicated the membranes were non-toxic. Cellular adhesion and proliferation were evaluated over 7 days for HFFF2 using resazurin assays and over 7 days for SH-SY5Y cells using the WST-1 assay, demonstrating cell proliferation. Differentiation assays using retinoic acid (RA) showed that SH-SY5Y cells differentiated into neuron-like cells on the scaffolds over 7 days. These findings suggest that the developed skin substitute is a promising candidate for treating skin injuries, addressing both physiological regeneration and functional restoration, with potential for future clinical applications. |
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| Autores principais: | Vilão, Alexandre Miguel dos Santos |
| Assunto: | chronic skin wounds tissue engineering electrospinning fish gelatin chitosan hyaluronic acid |
| Ano: | 2025 |
| País: | Portugal |
| Tipo de documento: | dissertação de mestrado |
| Tipo de acesso: | acesso aberto |
| Instituição associada: | Universidade Nova de Lisboa |
| Idioma: | inglês |
| Origem: | Repositório Institucional da UNL |
| Resumo: | Chronic wounds and burn injuries significantly impact global health, highlighting the limita- tions of current treatments that often fail to restore full skin functionality and sensitivity. This work presents the development of a dermal skin substitute composed of natural polymers—chitosan, fish gel- atin, and hyaluronic acid—designed to enhance skin regeneration and reinnervation. Electrospun scaffolds were fabricated using various combinations of these polymers and char- acterized for porosity (~48-84%), viscosity (~203-4150mPa·s), chemical composition using Fourier- transform infrared spectroscopy (FTIR), conductivity (~295-1130μS/cm), and morphology via scanning electron microscopy (SEM), which confirmed defect-free fibres with diameters of (~0.2-1.1μm). With a fibrous morphology mimicking the skin's native extracellular matrix, the scaffolds demonstrated ten- sile properties (~6-29 kPa) comparable to those of human skin. Further characterization included deg- radation rate (~0.2% over 64 days), swelling, and vapor transmission rate (~3700 g/m²·day). Biocompatibility was assessed using human fibroblasts (HFFF2) and neuroblastoma cells (SH- SY5Y). Cytotoxicity assays following ISO 10993-5 standards indicated the membranes were non-toxic. Cellular adhesion and proliferation were evaluated over 7 days for HFFF2 using resazurin assays and over 7 days for SH-SY5Y cells using the WST-1 assay, demonstrating cell proliferation. Differentiation assays using retinoic acid (RA) showed that SH-SY5Y cells differentiated into neuron-like cells on the scaffolds over 7 days. These findings suggest that the developed skin substitute is a promising candidate for treating skin injuries, addressing both physiological regeneration and functional restoration, with potential for future clinical applications. |
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