Publication
Tissue Engineering of Cardiac Arrhythmic Models For Drug Development
| Summary: | Cardiovascular diseases, particularly arrhythmias, remain a major global health challenge, characterized by high mortality rates and significant morbidity. The increasing prevalence of these conditions has spurred interest in innovative approaches to cardiac tissue engineering, aiming to develop functional tissues that can mimic the properties of native cardiac structures. This dissertation focuses on the development of bioinks designed to replicate the extracellular matrix (ECM) characteristics of cardiac tissue, specifically targeting conductivity and biocom- patibility to enhance cardiomyocyte maturation. The primary objective of this research was to create Gelatin Methacryloyl (GelMA) hydro- gels incorporated with conductive nanoparticles, facilitating the bioprinting of cardiac tissue models. These models are intended to emulate the electrical conductivity and anisotropic ar- chitecture of heart tissue, which are crucial for proper cardiac function. The study emphasizes the importance of these properties in promoting the growth and differentiation of cardiomy- ocytes, which are essential for effective tissue regeneration. Despite the challenges encountered, including the inability to successfully develop an in vitro model of arrhythmia, the findings contribute valuable insights into the potential of bioink formulations and bioprinting techniques in cardiac tissue engineering. In conclusion, while the goal of creating a functional in vitro arrhythmia model was not achieved, this dissertation lays the groundwork for future studies aimed at advancing cardiac tissue engineering. The proposed methodologies and materials offer promising avenues for developing cardiac models that could ultimately aid in drug development and therapeutic strategies for treating arrhythmias and other cardiovascular diseases. |
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| Main Authors: | Pinta, Carolina Ramos |
| Subject: | Arrhythmia Regeneration GelMA MXenes 3D Bioprinting Cardiomyocytes |
| Year: | 2024 |
| Country: | Portugal |
| Document type: | master thesis |
| Access type: | open access |
| Associated institution: | Universidade Nova de Lisboa |
| Language: | English |
| Origin: | Repositório Institucional da UNL |
| Summary: | Cardiovascular diseases, particularly arrhythmias, remain a major global health challenge, characterized by high mortality rates and significant morbidity. The increasing prevalence of these conditions has spurred interest in innovative approaches to cardiac tissue engineering, aiming to develop functional tissues that can mimic the properties of native cardiac structures. This dissertation focuses on the development of bioinks designed to replicate the extracellular matrix (ECM) characteristics of cardiac tissue, specifically targeting conductivity and biocom- patibility to enhance cardiomyocyte maturation. The primary objective of this research was to create Gelatin Methacryloyl (GelMA) hydro- gels incorporated with conductive nanoparticles, facilitating the bioprinting of cardiac tissue models. These models are intended to emulate the electrical conductivity and anisotropic ar- chitecture of heart tissue, which are crucial for proper cardiac function. The study emphasizes the importance of these properties in promoting the growth and differentiation of cardiomy- ocytes, which are essential for effective tissue regeneration. Despite the challenges encountered, including the inability to successfully develop an in vitro model of arrhythmia, the findings contribute valuable insights into the potential of bioink formulations and bioprinting techniques in cardiac tissue engineering. In conclusion, while the goal of creating a functional in vitro arrhythmia model was not achieved, this dissertation lays the groundwork for future studies aimed at advancing cardiac tissue engineering. The proposed methodologies and materials offer promising avenues for developing cardiac models that could ultimately aid in drug development and therapeutic strategies for treating arrhythmias and other cardiovascular diseases. |
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