Publicação
Development of a Conductive Bioink for 3D Bioprintig of Cardiac Tissues
| Resumo: | One of the leading causes of death we currently face is cardiovascular disease. Myocardial infarc- tion is an acute event known for damaging cardiac tissue due to the poor regeneration capacity of cardi- omyocytes. The most common therapeutic method is heart transplantation; however, it presents several limitations, such as donor shortage and the need for immunosuppressive therapy to avoid transplant rejection. To address these challenges, the field of tissue engineering has highlighted the need for bio- engineered scaffolds capable of mimicking the electrical and mechanical properties of the heart and promoting its regeneration. This project focuses on developing a conductive bioink composed of meth- acrylated gelatine (GelMA), hyaluronic acid (HA), and MXenes (MX) for the three-dimensional (3D) bioprinting of cardiac tissues. HA is known for its strong influence on cell growth, water retention, and biocompatibility. At the same time, GelMA provides structural support, and MXenes enhance the me- chanical and conductive properties of the construct. Combining these materials aims to create a bioink capable of replicating cardiac tissue's electrical properties and cellular functions while maintaining the necessary mechanical stability for cardiac tissue engineering. Most of the results demonstrate that the bioinks containing HA in their composition exhibited better printability and supported minimal cell viability, achieving a balance between elasticity and viscosity, and high conductivity (0.39469 S/m e 0.38378 S/m). The inclusion of MXenes had a significant impact on the mechanical strength and stiff- ness of the hydrogels, confirmed by the higher value of Young's Modulus (4914.90 ± 52.24 kPa). The development of this innovative bioink could lead to the creation of functional conductive scaffolds, which would significantly advance cardiac tissue engineering, particularly in the treatment of myocar- dial infarction and other heart-related conditions. |
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| Autores principais: | Kerfah, Lydia |
| Assunto: | Cardiac tissue engineering Methacrylated Gelatine Hyaluronic Acid MXenes 3D bioprinting |
| Ano: | 2024 |
| País: | Portugal |
| Tipo de documento: | dissertação de mestrado |
| Tipo de acesso: | acesso aberto |
| Instituição associada: | Universidade Nova de Lisboa |
| Idioma: | inglês |
| Origem: | Repositório Institucional da UNL |
| Resumo: | One of the leading causes of death we currently face is cardiovascular disease. Myocardial infarc- tion is an acute event known for damaging cardiac tissue due to the poor regeneration capacity of cardi- omyocytes. The most common therapeutic method is heart transplantation; however, it presents several limitations, such as donor shortage and the need for immunosuppressive therapy to avoid transplant rejection. To address these challenges, the field of tissue engineering has highlighted the need for bio- engineered scaffolds capable of mimicking the electrical and mechanical properties of the heart and promoting its regeneration. This project focuses on developing a conductive bioink composed of meth- acrylated gelatine (GelMA), hyaluronic acid (HA), and MXenes (MX) for the three-dimensional (3D) bioprinting of cardiac tissues. HA is known for its strong influence on cell growth, water retention, and biocompatibility. At the same time, GelMA provides structural support, and MXenes enhance the me- chanical and conductive properties of the construct. Combining these materials aims to create a bioink capable of replicating cardiac tissue's electrical properties and cellular functions while maintaining the necessary mechanical stability for cardiac tissue engineering. Most of the results demonstrate that the bioinks containing HA in their composition exhibited better printability and supported minimal cell viability, achieving a balance between elasticity and viscosity, and high conductivity (0.39469 S/m e 0.38378 S/m). The inclusion of MXenes had a significant impact on the mechanical strength and stiff- ness of the hydrogels, confirmed by the higher value of Young's Modulus (4914.90 ± 52.24 kPa). The development of this innovative bioink could lead to the creation of functional conductive scaffolds, which would significantly advance cardiac tissue engineering, particularly in the treatment of myocar- dial infarction and other heart-related conditions. |
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Atividades financiadas
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