Publicação
Stem cell bioprocessing strategies for cardiac regeneration
| Resumo: | "Cardiovascular diseases (CVD) are the leading cause of morbidity and mortality worldwide. Within CVD, myocardial infarction (MI) is associated with a massive and permanent loss of cardiomyocytes (CM). Advanced therapies based (...) numerous preclinical studies. Nonetheless, their clinical efficacy to improve the heart function remains elusive and controversial. The lack of validated and standardized cell culture protocols and characterization tools are among the contributing factors for this translational failure. Moreover, understanding the key cellular mechanisms underlying cardiac regeneration may also expose a multiplicity of therapeutic targets, paving the way for improved therapies by controlling cardiac tissue remodeling and/or to impose a pro-regenerative state. This thesis aimed at the implementation of novel strategies that could contribute for improved cardiac regenerative therapies. This work was divided into two main sections covering (A) the implementation of robust and scalable protocols for the expansion of human stem cells and (B) the interrogation of the functional role of a putative therapeutic molecule, CCBE1 (collagen and calcium- EGF binding domain 1), on cardiac commitment.(...)" |
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| Autores principais: | Marques Silva, Marta |
| Assunto: | regenerative therapies cardiac regeneration |
| Ano: | 2018 |
| País: | Portugal |
| Tipo de documento: | tese de doutoramento |
| Tipo de acesso: | acesso aberto |
| Instituição associada: | Universidade Nova de Lisboa |
| Idioma: | inglês |
| Origem: | Repositório Institucional da UNL |
| Resumo: | "Cardiovascular diseases (CVD) are the leading cause of morbidity and mortality worldwide. Within CVD, myocardial infarction (MI) is associated with a massive and permanent loss of cardiomyocytes (CM). Advanced therapies based (...) numerous preclinical studies. Nonetheless, their clinical efficacy to improve the heart function remains elusive and controversial. The lack of validated and standardized cell culture protocols and characterization tools are among the contributing factors for this translational failure. Moreover, understanding the key cellular mechanisms underlying cardiac regeneration may also expose a multiplicity of therapeutic targets, paving the way for improved therapies by controlling cardiac tissue remodeling and/or to impose a pro-regenerative state. This thesis aimed at the implementation of novel strategies that could contribute for improved cardiac regenerative therapies. This work was divided into two main sections covering (A) the implementation of robust and scalable protocols for the expansion of human stem cells and (B) the interrogation of the functional role of a putative therapeutic molecule, CCBE1 (collagen and calcium- EGF binding domain 1), on cardiac commitment.(...)" |
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