Publicação
INCORPORATION OF 4-AMINOPIRIDINE IN MEMBRANES FOR SKIN REINNERVATION
| Resumo: | Significant advances have been made in investigations regarding solutions for skin re- generation, however, challenges persist, particularly in restoring sensitivity and function to se- verely damaged skin. 4-aminopyridine (4-AP) has been identified as a drug that promotes rein- nervation, making it a promising candidate for dermal regeneration and reinnervation. In this study, 4-AP was incorporated for the first time into electrospun polymeric membranes of chi- tosan (CS), poly(ε-caprolactone) (PCL) and CS/PCL. This approach aims to contribute to the development of an innovative skin treatment, offering a potential alternative to conventional drug delivery methods. The incorporation of 4-AP resulted in an increase in porosity for the PCL and CS membranes, swelling ratio, and a reduction in fiber diameter to a level that could have a positive impact on wound healing. The PCL and CS/PCL membranes followed release kinetics consistent with the Higuchi model. Concentrations of 4-AP below 0.5 mM demon- strated no cytotoxicity in HFFF2, HaCaT, and SH-SY5Y cells, which were used as cell line models for fibroblasts, keratinocytes and neural cells, respectively. CS/PCL membranes supported the highest cell proliferation, as confirmed by adhesion/proliferation assays and fluorescence mi- croscopy images. 4-AP slowed the wound closure process in HaCaT and HFFF2 cells. Differen- tiation was observed in PCL/4-AP membranes with retinoic acid (RA), suggesting that 4-AP may enhance the effects of RA. However, additional replicates are needed to confirm that 4-AP induces SH-SY5Y cell differentiation on the membranes. |
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| Autores principais: | Ribeiro, Sofia Hassane |
| Assunto: | Skin regeneration 4-aminopyridine Wound healing assay Electrospinning Membranes Drug release |
| Ano: | 2024 |
| País: | Portugal |
| Tipo de documento: | dissertação de mestrado |
| Tipo de acesso: | acesso aberto |
| Instituição associada: | Universidade Nova de Lisboa |
| Idioma: | inglês |
| Origem: | Repositório Institucional da UNL |
| Resumo: | Significant advances have been made in investigations regarding solutions for skin re- generation, however, challenges persist, particularly in restoring sensitivity and function to se- verely damaged skin. 4-aminopyridine (4-AP) has been identified as a drug that promotes rein- nervation, making it a promising candidate for dermal regeneration and reinnervation. In this study, 4-AP was incorporated for the first time into electrospun polymeric membranes of chi- tosan (CS), poly(ε-caprolactone) (PCL) and CS/PCL. This approach aims to contribute to the development of an innovative skin treatment, offering a potential alternative to conventional drug delivery methods. The incorporation of 4-AP resulted in an increase in porosity for the PCL and CS membranes, swelling ratio, and a reduction in fiber diameter to a level that could have a positive impact on wound healing. The PCL and CS/PCL membranes followed release kinetics consistent with the Higuchi model. Concentrations of 4-AP below 0.5 mM demon- strated no cytotoxicity in HFFF2, HaCaT, and SH-SY5Y cells, which were used as cell line models for fibroblasts, keratinocytes and neural cells, respectively. CS/PCL membranes supported the highest cell proliferation, as confirmed by adhesion/proliferation assays and fluorescence mi- croscopy images. 4-AP slowed the wound closure process in HaCaT and HFFF2 cells. Differen- tiation was observed in PCL/4-AP membranes with retinoic acid (RA), suggesting that 4-AP may enhance the effects of RA. However, additional replicates are needed to confirm that 4-AP induces SH-SY5Y cell differentiation on the membranes. |
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