Publicação
Aspergillus ibericus biomass production by submerged fermentation of Codium tomentosum hydrolysate
| Resumo: | Seaweeds are potential feedstocks for biorefinery approaches, owing to their carbohydrate content, high biomass production and lack of lignin content, which makes saccharification more feasible. The seaweed sugar-rich hydrolysates can be used to produce alternative protein sources, such as mycoprotein, to meet the increasing global protein needs in a sustainable way. Therefore, this study aimed to produce biomass of the filamentous fungus Aspergillus ibericus MUM 03.49, a non-mycotoxin producer, from a seaweed hydrolysate. Firstly, hydrothermal pretreatment of Codium tomentosum was performed in an autoclave, followed by enzymatic hydrolysis using the commercial enzyme cocktail Cellic® Ctec 2 or a fungal enzyme crude extract. With the commercial enzyme cocktail, the maximum saccharification of cellulose and xylan was obtained when using 500 U g1 (around 75% and 30%, respectively), while when using the fungal enzyme extract, around 100% cellulose and 50% xylan conversion were achieved with 180 U xylanase g1. The obtained sugar-rich hydrolysate was used as the culture medium for A. ibericus growth in a stirred tank bioreactor. Several values of volumetric oxygen mass transfer coefficients (kLa) were used, from 20 to 66 h1, and a maximum biomass yield of 85% was achieved in batch cultures. The fungal biomass recovered contains 28% (w/w, dry mass) protein, 28% ash and 3% lipids. Aspergillus ibericus biomass has great potential as a protein alternative source for several applications, including in the food and feed industries. |
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| Autores principais: | Ferreira, Marta Alexandra Lages |
| Outros Autores: | Salgado, José Manuel Seara; Peres, Helena; Belo, Isabel |
| Assunto: | Aspergillus ibericus biorefinery hydrothermal and enzymatic hydrolysis mycoprotein seaweeds |
| Ano: | 2025 |
| País: | Portugal |
| Tipo de documento: | artigo |
| Tipo de acesso: | acesso restrito |
| Instituição associada: | Universidade do Minho |
| Idioma: | inglês |
| Origem: | RepositóriUM - Universidade do Minho |
| Resumo: | Seaweeds are potential feedstocks for biorefinery approaches, owing to their carbohydrate content, high biomass production and lack of lignin content, which makes saccharification more feasible. The seaweed sugar-rich hydrolysates can be used to produce alternative protein sources, such as mycoprotein, to meet the increasing global protein needs in a sustainable way. Therefore, this study aimed to produce biomass of the filamentous fungus Aspergillus ibericus MUM 03.49, a non-mycotoxin producer, from a seaweed hydrolysate. Firstly, hydrothermal pretreatment of Codium tomentosum was performed in an autoclave, followed by enzymatic hydrolysis using the commercial enzyme cocktail Cellic® Ctec 2 or a fungal enzyme crude extract. With the commercial enzyme cocktail, the maximum saccharification of cellulose and xylan was obtained when using 500 U g1 (around 75% and 30%, respectively), while when using the fungal enzyme extract, around 100% cellulose and 50% xylan conversion were achieved with 180 U xylanase g1. The obtained sugar-rich hydrolysate was used as the culture medium for A. ibericus growth in a stirred tank bioreactor. Several values of volumetric oxygen mass transfer coefficients (kLa) were used, from 20 to 66 h1, and a maximum biomass yield of 85% was achieved in batch cultures. The fungal biomass recovered contains 28% (w/w, dry mass) protein, 28% ash and 3% lipids. Aspergillus ibericus biomass has great potential as a protein alternative source for several applications, including in the food and feed industries. |
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