Bibliographic Details
| Summary: | Background: GPR125 belongs to the family of Adhesion G protein-coupled receptors (GPCRs). A single copy of GPR125 was found in many vertebrate genomes. We also identified a Drosophila sequence, DmCG15744, which shares a common ancestor with the entire Group III of Adhesion GPCRs, and also contains Ig, LRR and HBD domains which were observed in mammalian GPR125.Results: We found specific expression of GPR125 in cells of the choroid plexus using in situ hybridization and protein-specific antibodies and combined in situ/immunohistochemistry co-localization using cytokeratin, a marker specific for epithelial cells. Induction of inflammation by LPS did not change GPR125 expression. However, GPR125 expression was transiently increased ( almost 2-fold) at 4 h after traumatic brain injury (TBI) followed by a decrease ( approximately 4-fold) from 2 days onwards in the choroid plexus as well as increased expression ( 2-fold) in the hippocampus that was delayed until 1 day after injury.Conclusion: These findings suggest that GPR125 plays a functional role in choroidal and hippocampal response to injury. |
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| Main Authors: | Pickering, Chris |
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| Other Authors: | Hägglund, Maria; Szmydynger-Chodobska, Joanna; Marques, Fernanda; Palha, Joana Almeida; Waller, Linn; Chodobski, Adam; Fredriksson, Robert; Lagerström, Malin C.; Schiöth, Helgi B. |
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| Subject: | Animals Base Sequence Brain Injuries Choroid Plexus Gene Expression Profiling Hippocampus Humans Immunohistochemistry In Situ Hybridization Inflammation Keratins Lipopolysaccharides Male Membrane Glycoproteins Membrane Proteins Mice Platelet Glycoprotein GPIb-IX Complex RNA, Messenger Rats Receptors, G-Protein-Coupled Time Factors Up-Regulation |
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| Year: | 2008 |
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| Country: | Portugal |
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| Document type: | article |
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| Access type: | open access |
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| Associated institution: | Universidade do Minho |
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| Language: | English |
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| Origin: | RepositóriUM - Universidade do Minho |
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