Bibliographic Details
| Summary: | Burn wounds can lead to numerous severe complications including bacterial infections causing patient morbidity and mortality, mostly in low- and middle-income countries. The considerable increase of the microbial resistance against traditional antibiotics is leading towards alternative strategies to treat bacterial infections. Nisin Z is an antimicrobial peptide which exhibits a significant antibacterial activity against Gram-positive bacteria. Its efficacy against Gram-negative bacteria is limited, nonetheless it can be improved with the addition of surfactants, such as ethylenediaminetetraacetic acid (EDTA). The incorporation of peptide and other biomolecules within a biopolymer matrix provides protection maintaining their antimicrobial potential. Bacterial nanocellulose (BNC) has been widely used as wound dressings. Its impressive water retention capacity (> 99 %) and porosity are beneficial to manage wounds due to its potential to absorb exudates, providing a breathable and humid environment. In this work, the functionalization of BNC with Nisin Z (BNC-NZ) via vacuum filtration is reported. The entrapment of the peptide inside the BNC films was confirmed through morphological characterization using Attenuated Total Reflectance-Fourier Transform Infrared (ATR-FTIR) spectrometry. Typical absorbance peaks of Nisin Z are easily identifiable at 1647 cm-1 (amide group) and 1520 cm-1 (bending of primary amines). Thermal Gravimetric Analysis (TGA) suggested that Nisin Z did not interfere with the BNC matrix. The antimicrobial activity of Nisin Z against five of the most common bacteria found in burn wounds was verified by Minimum Bactericidal Concentration (MBC) ranging 8.0-256.0 µg/mL. Agar Diffusion and Shake Flask methods revealed the potential of BNC-NZ for prospective applications in burn wound dressings. |
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| Main Authors: | Melro, Liliana Sofia Silva Ferreira Pinto |
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| Other Authors: | Tavares, Tânia Daniela Eugénio; Padrão, Jorge; Dourado, Fernando; Gama, F. M.; Silva, Carla Manuela Costa; Antunes, Joana Carolina Simões; Felgueiras, Helena Prado; Zille, Andrea |
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| Subject: | Textile Antimicrobial peptides Bacterial nanocellulose Wound dressing Nisin Z Burn wounds Engenharia e Tecnologia::Engenharia dos Materiais |
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| Year: | 2022 |
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| Country: | Portugal |
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| Document type: | conference poster |
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| Access type: | open access |
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| Associated institution: | Universidade do Minho |
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| Language: | English |
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| Origin: | RepositóriUM - Universidade do Minho |
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