Publicação
Phaeodactylum tricornutum biorefinery: a sustainable approach to valorize microalgae biomass towards biomolecules recovery
| Resumo: | Microalgae superior ability to sustain harsh, stressing or limiting conditions is widely known, while being also a unique source of specific and valuable biocompounds. Microalgae extracts’ potential has been well recognized for their interesting functional and bioactive properties, showing specially interesting features for skin care products’ manufacturing mainly as thickening, water-binding and antioxidant agents. The expanding interest in addressing a more sustainable and eco-friendly product development results in a growing community searching for natural-based formulations. However, microalgae potential and usefulness are dependent on the extraction techniques applied. The selection of the preferential method presents a major impact on the final product quality, which should exceed costumer’s requests, while simultaneously offering an economically appealing process. Thus, this thesis aimed at acknowledging Phaeodactylum tricornutum potential, by ultimately providing important information for the formulation of an algae-based cosmeceutical product. The present work focused on achieving two different main fractions accountable for bioactive and technological functions, underlying P. tricornutum growth conditions impact and designing the extraction methodology, including ohmic heating (OH) as a potential alternative to conventional extraction processes. Firstly, an adaptive laboratory evolution (ALE) taking advantage of UV-C mutagenesis was performed in P. tricornutum cultures. Although the results did not allow a clear positive impact in global extracts’ antioxidant activity, it presented as a potentially useful tool to enhance lipidic extraction. In order to explore the OH-based and conventional extraction implications on the microalgae extracts properties, water and different ethanol/water mixtures were used as solvents, addressing a polarity spectrum. P. tricornutum functionality was evaluated by relating the extracts composition (assessed by intrinsic fluorescence analysis, SDS-PAGE electrophoresis, FTIR and gel permeation chromatography) with their thermal, rheological and emulsifying behavior. Bioactive potential was analyzed by three different antioxidant activity measuring tests (FRAP, ABTS and DPPH) and a viability assay in normal and cancer cell lines. This diatom extracts showed promising technological and bioactive potential, with interesting gelling, emulsification and antioxidant capacities. Extracts showed different biochemical and performance profiles, depending on the solvent used. Generally, no significant effects were observed for OH application, having this process lower energy consumptions. In conclusion, the major goals of the present work were successfully accomplished, underlining P. tricornutum extracts potentiality not only for cosmeceutical industry, but also for functional foods development. |
|---|---|
| Autores principais: | Ferreira, Catarina Castro |
| Assunto: | Bioactivity Extraction Functional properties Ohmic heating Phaeodactylum tricornutum Engenharia e Tecnologia |
| Ano: | 2021 |
| País: | Portugal |
| Tipo de documento: | dissertação de mestrado |
| Tipo de acesso: | acesso aberto |
| Instituição associada: | Universidade do Minho |
| Idioma: | inglês |
| Origem: | RepositóriUM - Universidade do Minho |
| Resumo: | Microalgae superior ability to sustain harsh, stressing or limiting conditions is widely known, while being also a unique source of specific and valuable biocompounds. Microalgae extracts’ potential has been well recognized for their interesting functional and bioactive properties, showing specially interesting features for skin care products’ manufacturing mainly as thickening, water-binding and antioxidant agents. The expanding interest in addressing a more sustainable and eco-friendly product development results in a growing community searching for natural-based formulations. However, microalgae potential and usefulness are dependent on the extraction techniques applied. The selection of the preferential method presents a major impact on the final product quality, which should exceed costumer’s requests, while simultaneously offering an economically appealing process. Thus, this thesis aimed at acknowledging Phaeodactylum tricornutum potential, by ultimately providing important information for the formulation of an algae-based cosmeceutical product. The present work focused on achieving two different main fractions accountable for bioactive and technological functions, underlying P. tricornutum growth conditions impact and designing the extraction methodology, including ohmic heating (OH) as a potential alternative to conventional extraction processes. Firstly, an adaptive laboratory evolution (ALE) taking advantage of UV-C mutagenesis was performed in P. tricornutum cultures. Although the results did not allow a clear positive impact in global extracts’ antioxidant activity, it presented as a potentially useful tool to enhance lipidic extraction. In order to explore the OH-based and conventional extraction implications on the microalgae extracts properties, water and different ethanol/water mixtures were used as solvents, addressing a polarity spectrum. P. tricornutum functionality was evaluated by relating the extracts composition (assessed by intrinsic fluorescence analysis, SDS-PAGE electrophoresis, FTIR and gel permeation chromatography) with their thermal, rheological and emulsifying behavior. Bioactive potential was analyzed by three different antioxidant activity measuring tests (FRAP, ABTS and DPPH) and a viability assay in normal and cancer cell lines. This diatom extracts showed promising technological and bioactive potential, with interesting gelling, emulsification and antioxidant capacities. Extracts showed different biochemical and performance profiles, depending on the solvent used. Generally, no significant effects were observed for OH application, having this process lower energy consumptions. In conclusion, the major goals of the present work were successfully accomplished, underlining P. tricornutum extracts potentiality not only for cosmeceutical industry, but also for functional foods development. |
|---|
Atividades financiadas
Não foram encontrados projetos financiados.