Publication
From honeycomb- to microsphere-patterned surfaces of poly(lactic acid) and a starch-poly(lactic acid) blend via the breath figure method
| Summary: | Background: This study investigated the preparation of ordered patterned surfaces and/or microspheres from a natural-based polymer, using the breath figure and reverse breath figure methods. Methods: Poly(D,L-lactic acid) and starch poly(lactic acid) solutions were precipitated in different conditions â namely, polymer concentration, vapor atmosphere temperature and substrate â to evaluate the effect of these conditions on the morphology of the precipitates obtained. Results: The possibility of fine-tuning the properties of the final patterns simply by changing the vapor atmo- sphere was also demonstrated here using a range of compositions of the vapor phase. Porous films or discrete particles are formed when the differences in surface tension determine the ability of polymer solution to sur- round water droplets or methanol to surround polymer droplets, respectively. In vitro cytotoxicity was assessed applying a simple standard protocol to evaluate the possibility to use these materials in biomedical applications. Moreover, fluorescent microscopy images showed a good interaction of cells with the material, which were able to adhere on the patterned surfaces after 24 hours in culture. Conclusions: The development of patterned surfaces using the breath figure method was tested in this work for the preparation of both poly(lactic acid) and a blend containing starch and poly(lactic acid). The potential of these films to be used in the biomedical area was confirmed by a preliminary cytotoxicity test and by morphological observation of cell adhesion. |
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| Main Authors: | Duarte, Ana Rita C. |
| Other Authors: | Maniglio, D.; Sousa, Nuno; Mano, J. F.; Reis, R. L.; Migliaresi, C. |
| Subject: | Biomaterials Breath figures natural polymers Patterns Poly(lactic acid) Starch |
| Year: | 2017 |
| Country: | Portugal |
| Document type: | article |
| Access type: | restricted access |
| Associated institution: | Universidade do Minho |
| Language: | English |
| Origin: | RepositóriUM - Universidade do Minho |
| Summary: | Background: This study investigated the preparation of ordered patterned surfaces and/or microspheres from a natural-based polymer, using the breath figure and reverse breath figure methods. Methods: Poly(D,L-lactic acid) and starch poly(lactic acid) solutions were precipitated in different conditions â namely, polymer concentration, vapor atmosphere temperature and substrate â to evaluate the effect of these conditions on the morphology of the precipitates obtained. Results: The possibility of fine-tuning the properties of the final patterns simply by changing the vapor atmo- sphere was also demonstrated here using a range of compositions of the vapor phase. Porous films or discrete particles are formed when the differences in surface tension determine the ability of polymer solution to sur- round water droplets or methanol to surround polymer droplets, respectively. In vitro cytotoxicity was assessed applying a simple standard protocol to evaluate the possibility to use these materials in biomedical applications. Moreover, fluorescent microscopy images showed a good interaction of cells with the material, which were able to adhere on the patterned surfaces after 24 hours in culture. Conclusions: The development of patterned surfaces using the breath figure method was tested in this work for the preparation of both poly(lactic acid) and a blend containing starch and poly(lactic acid). The potential of these films to be used in the biomedical area was confirmed by a preliminary cytotoxicity test and by morphological observation of cell adhesion. |
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