Publicação
Assessing the molecular fingerprint of T lymphocyte activation
| Resumo: | Methods to evaluate Lymphocyte T function, are very important in clinical context, namely in patients with primary and secondary immunodeficiencies, bone marrow or hematopoietic stem cell transplantation, organ transplant and immunosuppressive therapy. However, the conventional monitoring techniques are based on laborious, expensive methods. In the present work, it was evaluated the potential of an infrared spectroscopy based-method, in the assessment of lymphocyte T function after mitogen activation. It was possible to discriminate, either by principal component analysis either by hierarchical cluster analysis of second derivative spectra of lymphocytes T, between resting T cells and activated T cells. The infrared spectroscopy-based method presents therefore a high potential for a new diagnostic tool of the T cell alloreactivity. |
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| Autores principais: | Ramalhete, Luís |
| Outros Autores: | Calado, Cecília |
| Assunto: | T cell Phytohemagglutinin Infrared spectroscopy |
| Ano: | 2019 |
| País: | Portugal |
| Tipo de documento: | documento de conferência |
| Tipo de acesso: | acesso restrito |
| Instituição associada: | Universidade Politécnica de Lisboa |
| Idioma: | inglês |
| Origem: | Repositório Científico da Universidade Politécnica de Lisboa |
| Resumo: | Methods to evaluate Lymphocyte T function, are very important in clinical context, namely in patients with primary and secondary immunodeficiencies, bone marrow or hematopoietic stem cell transplantation, organ transplant and immunosuppressive therapy. However, the conventional monitoring techniques are based on laborious, expensive methods. In the present work, it was evaluated the potential of an infrared spectroscopy based-method, in the assessment of lymphocyte T function after mitogen activation. It was possible to discriminate, either by principal component analysis either by hierarchical cluster analysis of second derivative spectra of lymphocytes T, between resting T cells and activated T cells. The infrared spectroscopy-based method presents therefore a high potential for a new diagnostic tool of the T cell alloreactivity. |
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