Publicação
The influence of short-chain fatty acids on the survival and virulence of Arcobacter butzleri
| Resumo: | Aims: Arcobacter butzleri, a widespread bacterium linked to gastrointestinal disease, can bypass host colonization resistance mechanisms; however, its response to short-chain fatty acids (SCFAs) remains poorly understood. This study investigated the impact of SCFAs on A. butzleri ’s survival and virulence. Methods and results: Eight A. butzleri isolates were assessed under varying concentrations of individual SCFAs and mixtures (m-SCFAs). Higher SCFAs concentrations inhibited bacterial growth in a strain-dependent manner. Transcript analysis of putative virulence genes revealed upregula- tion of ciaB and flaA across most m-SCFAs concentrations, while luxS expression increased at 90 mM. SCFAs generally reduced bacterial motility, with sodium propionate reducing motility but enhancing biofilm-forming ability in the model strain. Additionally, SCFAs exposure decreased the ability of A. butzleri to adhere to and invade the Caco-2 intestinal epithelial cell line. Whole-genome sequencing of the eight A. butzleri isolates revealed extensive genetic diversity, particularly in virulence- and stress-associated genes, although consistent genot ype/phenot ype correlations were not observed. Conclusions: Altogether, these findings demonstrate that SCFAs modulate A. butzleri survival and virulence, providing novel insights into their significance in shaping pathogen behaviour and host-pathogen interactions. |
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| Autores principais: | Fonseca, Inês M. |
| Outros Autores: | Mateus, Cristiana; Vieira, Alexandre; Domingues, Fernanda; Manageiro, Vera; Oleastro, Mónica; Ferreira, Susana |
| Assunto: | Arcobacter Butzleri Adhesion and Invasion Biofilms Motility Short-Chain Fatty Acids Virulence Genes Resistência aos Antimicrobianos Infecções Gastrointestinais |
| Ano: | 2025 |
| País: | Portugal |
| Tipo de documento: | artigo |
| Tipo de acesso: | acesso restrito |
| Instituição associada: | Instituto Nacional de Saúde Doutor Ricardo Jorge |
| Idioma: | inglês |
| Origem: | Repositório Científico do Instituto Nacional de Saúde |
| Resumo: | Aims: Arcobacter butzleri, a widespread bacterium linked to gastrointestinal disease, can bypass host colonization resistance mechanisms; however, its response to short-chain fatty acids (SCFAs) remains poorly understood. This study investigated the impact of SCFAs on A. butzleri ’s survival and virulence. Methods and results: Eight A. butzleri isolates were assessed under varying concentrations of individual SCFAs and mixtures (m-SCFAs). Higher SCFAs concentrations inhibited bacterial growth in a strain-dependent manner. Transcript analysis of putative virulence genes revealed upregula- tion of ciaB and flaA across most m-SCFAs concentrations, while luxS expression increased at 90 mM. SCFAs generally reduced bacterial motility, with sodium propionate reducing motility but enhancing biofilm-forming ability in the model strain. Additionally, SCFAs exposure decreased the ability of A. butzleri to adhere to and invade the Caco-2 intestinal epithelial cell line. Whole-genome sequencing of the eight A. butzleri isolates revealed extensive genetic diversity, particularly in virulence- and stress-associated genes, although consistent genot ype/phenot ype correlations were not observed. Conclusions: Altogether, these findings demonstrate that SCFAs modulate A. butzleri survival and virulence, providing novel insights into their significance in shaping pathogen behaviour and host-pathogen interactions. |
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