Publication
Development and validation of human placenta-derived hydrogels for 3D cell culture
| Summary: | Three-dimensional (3D) cell culture platforms have emerged as potential alternatives to the two-dimensional platforms traditionally used, due to their ability to recapitulate the native microenvironment more accurately. Inspired by the extracellular matrix (ECM) of tissues researchers are increasingly focus on developing models that mimic this structure. Hydrogels are hydrophilic polymeric networks capable of retaining large amounts of water, allowing nutrient and oxygen exchange, as well as cell adhesion and proliferation. Currently, Matrigel® is the most widely used commercial biomaterial for 3D cell culture, but due to its animal origin it is not efficient at accurately recapitulating the human microenvironment, resulting in a reduction in the clinical value of the in vitro results. In 2018, Metatissue® saw this gap as an opportunity to integrate into the market cell culture platforms derived from human blood and perinatal tissues. Hydrogels derived from amniotic membrane (AM), capable of effectively recapitulate the microenvironment of the human body, are being optimized for future commercialization. However, the production yields of the product per AM are very low. |
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| Main Authors: | Oliveira, Maria Inês Martins |
| Subject: | 3D cell culture Extracellular matrix Natural biomaterials Hydrogels Perinatal tissues Placenta Humanized models |
| Year: | 2023 |
| Country: | Portugal |
| Document type: | master thesis |
| Access type: | embargoed access |
| Associated institution: | Universidade de Aveiro |
| Language: | English |
| Origin: | RIA - Repositório Institucional da Universidade de Aveiro |
| Summary: | Three-dimensional (3D) cell culture platforms have emerged as potential alternatives to the two-dimensional platforms traditionally used, due to their ability to recapitulate the native microenvironment more accurately. Inspired by the extracellular matrix (ECM) of tissues researchers are increasingly focus on developing models that mimic this structure. Hydrogels are hydrophilic polymeric networks capable of retaining large amounts of water, allowing nutrient and oxygen exchange, as well as cell adhesion and proliferation. Currently, Matrigel® is the most widely used commercial biomaterial for 3D cell culture, but due to its animal origin it is not efficient at accurately recapitulating the human microenvironment, resulting in a reduction in the clinical value of the in vitro results. In 2018, Metatissue® saw this gap as an opportunity to integrate into the market cell culture platforms derived from human blood and perinatal tissues. Hydrogels derived from amniotic membrane (AM), capable of effectively recapitulate the microenvironment of the human body, are being optimized for future commercialization. However, the production yields of the product per AM are very low. |
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Funded activities
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