Publicação
Adaptation of Scheffersomyces stipitis to hardwood spent sulfite liquor by evolutionary engineering
| Resumo: | Hardwood spent sulfite liquor (HSSL) is a by-product of acid sulfite pulping process that is rich in xylose, a monosaccharide that can be fermented to ethanol by Scheffersomyces stipitis. However, HSSL also contains acetic acid and lignosulfonates that are inhibitory compounds of yeast growth. The main objective of this study was the use of an evolutionary engineering strategy to obtain variants of S. stipitis with increased tolerance to HSSL inhibitors while maintaining the ability to ferment xylose to ethanol. |
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| Autores principais: | Pereira, Susana R |
| Outros Autores: | Sanchez i Nogue, Violeta; Frazao, Cludio J R; Serafim, Luisa S; Gorwa-Grauslund, Marie F; Xavier, Ana M R B |
| Assunto: | Hardwood spent sulfite liquor (HSSL) Scheffersomyces stipitis Lignocellulosic inhibitors Evolutionary engineering Bioethanol |
| Ano: | 2015 |
| País: | Portugal |
| Tipo de documento: | artigo |
| Tipo de acesso: | acesso aberto |
| Instituição associada: | Universidade de Aveiro |
| Idioma: | inglês |
| Origem: | RIA - Repositório Institucional da Universidade de Aveiro |
| Resumo: | Hardwood spent sulfite liquor (HSSL) is a by-product of acid sulfite pulping process that is rich in xylose, a monosaccharide that can be fermented to ethanol by Scheffersomyces stipitis. However, HSSL also contains acetic acid and lignosulfonates that are inhibitory compounds of yeast growth. The main objective of this study was the use of an evolutionary engineering strategy to obtain variants of S. stipitis with increased tolerance to HSSL inhibitors while maintaining the ability to ferment xylose to ethanol. |
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