Publication
Extraction of phenolic compounds from rosemary using choline chloride – based Deep Eutectic Solvents
| Summary: | Deep eutectic solvents (DES) have been explored as an alternative to hazardous solvents to extract natural bioactive compounds. In this work, choline chloride-based DES (neat and hydrated) were assessed in the extraction of phenolics from rosemary leaves. The DES studied on an initial screening showed a total phenolic content (TPC) up to 220% higher than the control. The solubilities of the main rosemary biocompounds, estimated by COSMO-RS, showed a positive correlation to TPC. Choline chloride:1,2-propanediol (CPH) was selected for further optimization. At the optimal conditions (65 °C, liquid: solid ratio of 40:1 and 50 %wt of water), the antioxidant activity and TPC were 80 mg of Trolox equivalent/g and 78 mg of gallic acid equivalent/g, respectively. The antimicrobial activity of the optimized extract revealed inhibition of 39–51% to all bacteria tested. In summary, an extract with good antioxidant and antimicrobial activities was obtained from rosemary leaves using CPH as solvent. |
|---|---|
| Main Authors: | Wojeicchowski, José Pedro |
| Other Authors: | Marques, Caroline; Igarashi-Mafra, Luciana; Coutinho, João A.P.; Mafra, Marcos R. |
| Subject: | Solubility Carnosic acid Carnosol Rosmarinic acid COSMO-RS |
| Year: | 2021 |
| Country: | Portugal |
| Document type: | article |
| Access type: | open access |
| Associated institution: | Universidade de Aveiro |
| Language: | English |
| Origin: | RIA - Repositório Institucional da Universidade de Aveiro |
| Summary: | Deep eutectic solvents (DES) have been explored as an alternative to hazardous solvents to extract natural bioactive compounds. In this work, choline chloride-based DES (neat and hydrated) were assessed in the extraction of phenolics from rosemary leaves. The DES studied on an initial screening showed a total phenolic content (TPC) up to 220% higher than the control. The solubilities of the main rosemary biocompounds, estimated by COSMO-RS, showed a positive correlation to TPC. Choline chloride:1,2-propanediol (CPH) was selected for further optimization. At the optimal conditions (65 °C, liquid: solid ratio of 40:1 and 50 %wt of water), the antioxidant activity and TPC were 80 mg of Trolox equivalent/g and 78 mg of gallic acid equivalent/g, respectively. The antimicrobial activity of the optimized extract revealed inhibition of 39–51% to all bacteria tested. In summary, an extract with good antioxidant and antimicrobial activities was obtained from rosemary leaves using CPH as solvent. |
|---|
Funded activities
No funded projects found.