Bibliographic Details
| Summary: | Aim: Propose a nanoparticle for neuron-targeted retrograde gene delivery and describe a microfluidic-based culture system to provide insight into vector performance and safety. Methods: Using compartmentalized neuron cultures we dissected nanoparticle bioactivity upon delivery taking advantage of (quantitative) bioimaging tools. Results: Targeted and nontargeted nanoparticles were internalized at axon terminals and retrogradely transported to cell bodies at similar average velocities but the former have shown an axonal flux 2.7-times superior to nontargeted nanoparticles, suggesting an improved cargo-transportation efficiency. The peripheral administration of nanoparticles to axon terminals is nontoxic as compared with their direct administration to the cell body or whole neuron. Conclusion: A neuron-targeted nanoparticle system was put forward. Microfluidic-based neuron cultures are proposed as a powerful tool to investigate nanoparticle bio-performance. |
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| Main Authors: | Lopes, C |
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| Other Authors: | Gomes, C; Neto, E; Sampaio, P; Aguiar, P; Pêgo, AP |
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| Subject: | gene delivery microfluidics targeted nanoparticles |
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| Year: | 2016 |
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| Country: | Portugal |
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| Document type: | article |
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| Access type: | open access |
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| Associated institution: | Universidade do Porto |
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| Language: | English |
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| Origin: | Repositório Aberto da Universidade do Porto |
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