Publicação
Determination of Target Pesticide Residues in Tropical Fruits Employing Matrix Solid-Phase Dispersion (MSPD) Extraction Followed by High Resolution Gas Chromatography
| Resumo: | A simple method based on matrix solid-phase dispersion (MSPD) extraction and gas chromatography with electron-capture detection (GC-ECD) is employed to determine pesticide residues in the following tropical fruits: banana, mango, melon, papaya and pineapple. In the MSPD proposed method, C18, silica gel and ethyl acetate presented the best results in the extraction, clean-up and elution steps, respectively. Spiked blank samples were used to minimize the matrix effect in the chromatographic determination. The validation process was conducted at three different concentration levels of spiked samples (0.50-2.50 µg g-1) in within-a-day and in among days assays. The limits of detection for the pesticides ranged from 4.0 to 23 µg kg-1. The method showed acceptable selectivity, coefficients of correlation higher than 0.997, and recovery between 76-105%. The within-a-day precision was assessed through the relative standard deviation (2.8-19%) for the different levels of spiked samples tested. |
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| Autores principais: | Freitas,Silvia S. |
| Outros Autores: | Serafim,Felipe A. T.; Lanças,Fernando M. |
| Assunto: | matrix solid-phase dispersion pesticide residues method validation GC-ECD |
| Ano: | 2018 |
| País: | Brasil |
| Tipo de documento: | artigo |
| Tipo de acesso: | acesso aberto |
| Instituição associada: | IBICT |
| Idioma: | inglês |
| Origem: | Oasisbr |
| Resumo: | A simple method based on matrix solid-phase dispersion (MSPD) extraction and gas chromatography with electron-capture detection (GC-ECD) is employed to determine pesticide residues in the following tropical fruits: banana, mango, melon, papaya and pineapple. In the MSPD proposed method, C18, silica gel and ethyl acetate presented the best results in the extraction, clean-up and elution steps, respectively. Spiked blank samples were used to minimize the matrix effect in the chromatographic determination. The validation process was conducted at three different concentration levels of spiked samples (0.50-2.50 µg g-1) in within-a-day and in among days assays. The limits of detection for the pesticides ranged from 4.0 to 23 µg kg-1. The method showed acceptable selectivity, coefficients of correlation higher than 0.997, and recovery between 76-105%. The within-a-day precision was assessed through the relative standard deviation (2.8-19%) for the different levels of spiked samples tested. |
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