Publication
Evaluation of Monolithic and Core-Shell Columns for Separation of Triazine Herbicides by Reversed Phase High Performance Liquid Chromatography
| Summary: | Monolithic and core-shell columns were comparatively evaluated for separation of deisopropylatrazine (DIA), deethylatrazine (DEA), hydroxyatrazine (HAT), simazine (SIM) and atrazine (ATR) under similar conditions of elution, flow rate and sample volume. Although both columns provided separation of the analytes with resolution > 1.5, the core-shell column exhibited better efficiency, leading to narrower peaks. It has height equivalent to the theoretical plates (HETP) for DEA, DIA and HAT, eluted under isocratic elution, about 2.5 times smaller than those of the monolithic column. A soil sample was studied by ultrasound assisted extraction with 80:20 (v/v) acetonitrile:water, revealing the presence of HAT and ATR. This sample was enriched with 0.25, 0.50 and 1.0 mg kg-1 of the five triazine compounds and analyzed, leading to recovery percentages between 45 and 86%. |
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| Main Authors: | Urio,Ricardo P. |
| Other Authors: | Masini,Jorge C. |
| Subject: | atrazine simazine ultrasound extraction monolithic column core-shell column |
| Year: | 2015 |
| Country: | Brazil |
| Document type: | article |
| Access type: | open access |
| Associated institution: | IBICT |
| Language: | English |
| Origin: | Oasisbr |
| Summary: | Monolithic and core-shell columns were comparatively evaluated for separation of deisopropylatrazine (DIA), deethylatrazine (DEA), hydroxyatrazine (HAT), simazine (SIM) and atrazine (ATR) under similar conditions of elution, flow rate and sample volume. Although both columns provided separation of the analytes with resolution > 1.5, the core-shell column exhibited better efficiency, leading to narrower peaks. It has height equivalent to the theoretical plates (HETP) for DEA, DIA and HAT, eluted under isocratic elution, about 2.5 times smaller than those of the monolithic column. A soil sample was studied by ultrasound assisted extraction with 80:20 (v/v) acetonitrile:water, revealing the presence of HAT and ATR. This sample was enriched with 0.25, 0.50 and 1.0 mg kg-1 of the five triazine compounds and analyzed, leading to recovery percentages between 45 and 86%. |
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