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Production of yeast extract from whey using Kluyveromyces marxianus

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Bibliographic Details
Summary:The yeast Kluyveromyces marxianus CBS 6556 was grown on whey to produce nucleotide-rich yeast extracts. Thermal treatments of cells at 35 or 50ºC for 15-30h resulted in yeast extracts containing about 20 g/L protein, with only the second treatment resulting in the presence of small amounts of RNA. In contrast, autolysis in buffered solution was the unique treatment that resulted in release of high amounts of intracellular RNA, being, therefore, the better procedure to produce 5'-nucletide rich extract with K. marxianus.
Main Authors:Revillion,Jean P. de Palma
Other Authors:Brandelli,Adriano; Ayub,Marco A. Záchia
Subject:Cheese whey yeast extract 5'-nucleotide food flavour enhancer
Year:2003
Country:Brazil
Document type:article
Access type:open access
Associated institution:IBICT
Language:English
Origin:Oasisbr
Description
Summary:The yeast Kluyveromyces marxianus CBS 6556 was grown on whey to produce nucleotide-rich yeast extracts. Thermal treatments of cells at 35 or 50ºC for 15-30h resulted in yeast extracts containing about 20 g/L protein, with only the second treatment resulting in the presence of small amounts of RNA. In contrast, autolysis in buffered solution was the unique treatment that resulted in release of high amounts of intracellular RNA, being, therefore, the better procedure to produce 5'-nucletide rich extract with K. marxianus.

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