Publicação
Identificação e perfil de resistência a antimicrobianos de Staphylococcus pseudintermedius isolados de piodermite canina
| Resumo: | Pyoderma is one of the most common skin disorders in dogs and Staphylococcus pseudintermedius is the bacterium that is most often isolated from lesions. During recent years a major concern in veterinary dermatology has been the increasing number of methicillin resistant Staphylococcus spp. isolates, that are resistant to several antimicrobials used in dermatological clinic. Infections caused by these resistant bacteria are a major cause of morbidity in pets and can be a source of transmission to humans. The objectives of this work are to review the literature on Staphylococcus pseudintermedius and the resistance in these bacteria. It is also to isolate and identify through biochemical and molecular biology tests, bacteria that causes canine pyoderma, determining their resistance profile to antimicrobials commonly used for this condition by antimicrobial susceptibility test and by the presence of the resistance gene, mecA. Seventy five bacterial isolates were obtained from the 25 animals selected to this study. A total of 97.3% of the isolates submitted to conventional phenotypic tests and API Staph Kit and 96% of those submitted to PCR were identified as Staphylococcus pseudintermedius. Of the 72 isolates submitted to PCR for the identification of the mecA gene, only four did not have this resistance gene. This study alerts to the high number of resistant Staphylococcus pseudintermedius in Brazil, and to the need of performing antimicrobial susceptibility tests to determine the best therapeutic approach, preventing the emergence of multi-resistant bacteria. |
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| Autores principais: | Silva, Elisa Bourguignon Dias da |
| Assunto: | Cão Dermatose Staphylococcus pseudintermedius Dog Dermatosis Staphylococcus pseudintermedius CNPQ::CIENCIAS AGRARIAS::MEDICINA VETERINARIA::CLINICA E CIRURGIA ANIMAL |
| Ano: | 2015 |
| País: | Brasil |
| Tipo de documento: | dissertação de mestrado |
| Tipo de acesso: | acesso aberto |
| Instituição associada: | IBICT |
| Idioma: | português |
| Origem: | Oasisbr |
| Resumo: | Pyoderma is one of the most common skin disorders in dogs and Staphylococcus pseudintermedius is the bacterium that is most often isolated from lesions. During recent years a major concern in veterinary dermatology has been the increasing number of methicillin resistant Staphylococcus spp. isolates, that are resistant to several antimicrobials used in dermatological clinic. Infections caused by these resistant bacteria are a major cause of morbidity in pets and can be a source of transmission to humans. The objectives of this work are to review the literature on Staphylococcus pseudintermedius and the resistance in these bacteria. It is also to isolate and identify through biochemical and molecular biology tests, bacteria that causes canine pyoderma, determining their resistance profile to antimicrobials commonly used for this condition by antimicrobial susceptibility test and by the presence of the resistance gene, mecA. Seventy five bacterial isolates were obtained from the 25 animals selected to this study. A total of 97.3% of the isolates submitted to conventional phenotypic tests and API Staph Kit and 96% of those submitted to PCR were identified as Staphylococcus pseudintermedius. Of the 72 isolates submitted to PCR for the identification of the mecA gene, only four did not have this resistance gene. This study alerts to the high number of resistant Staphylococcus pseudintermedius in Brazil, and to the need of performing antimicrobial susceptibility tests to determine the best therapeutic approach, preventing the emergence of multi-resistant bacteria. |
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