Bibliographic Details
| Summary: | A label-free optical immunosensor was developed based on surface plasmon resonance (SPR) method for rapid and selective detection of Brucella melitensis. This immunosensor was constructed by immobilizing capture antibody on 11-mercaptoundecanoic acid modified gold disk. This fabricated immunosensor detected B. melitensis at a concentration range from 103 to 107 cell mL-1 (R2 = 0.998) and a detection limit of 100 cell mL-1. Additionally, the kinetic equilibrium dissociation constant (KD) for assessment of the interaction affinity was calculated as 1.1 × 10-9 mol L-1 that can be considered as high affinity interaction. This SPR immunosensor provided advantages in term of fast response, label free and accurate detection of B. melitensis in analytical systems. |
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| Main Authors: | Saberi,Fatemeh |
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| Other Authors: | Kamali,Mehdi; Taheri,Ramezan A.; Ramandi,Mahdi F.; Bagdeli,Samira; Mirnejad,Reza |
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| Subject: | Brucella surface plasmon resonance kinetics biosensor |
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| Year: | 2016 |
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| Country: | Brazil |
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| Document type: | article |
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| Access type: | open access |
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| Associated institution: | IBICT |
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| Language: | English |
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| Origin: | Oasisbr |
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