Detalhes bibliográficos
| Resumo: | PURPOSE: To assess S. aureus in vitro viability after the exposure to ultraviolet light A (UVA) and riboflavin (B2). METHODS: Samples of S. aureus in 96 well plates (in triplicate) were exposed to riboflavin (B2) and ultraviolet light A (365 nm wavelength) at a power density of 3 mW/cm², 8 mm spot diameter, for 30 minutes. Control groups were prepared as well in triplicate: blank control, ultraviolet light A only, riboflavin only and dead bacteria Control. The bacterial viability was measured using fluorescent microscopy. In order to investigate the occurrence of "viable but non-culturable" microorganisms after treatment, the cell viability was also investigated by plate culture procedure onto a broth medium. Statistical analysis was performed using the triplicate values from each experimental condition. RESULTS: No difference was observed among the treatment group and the control samples (p=1). CONCLUSION: The combination of riboflavin 0.1% and ultraviolet light A at 365 nm did not exhibit antimicrobial activity against oxacillin susceptible S. aureus. |
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| Autores principais: | Kashiwabuchi,Renata Tiemi |
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| Outros Autores: | Khan,Yasin; Carvalho,Fabio Ramos de Souza; Hirai,Flavio; Campos,Mauro Silveira; McDonnell,Peter John |
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| Assunto: | Keratitis Riboflavin Corneal ulcer Staphylococcus aureus Ultraviolet rays Cross-linking reagents |
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| Ano: | 2012 |
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| País: | Brasil |
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| Tipo de documento: | artigo |
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| Tipo de acesso: | acesso aberto |
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| Instituição associada: | IBICT |
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| Idioma: | inglês |
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| Origem: | Oasisbr |
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