Publication

Monitoring of 17 β-estradiol in raw and treated samples of wastewater treatment plants

Bibliographic Details
Summary:	Nowadays, monitoring the concentration levels of estrogens in treated wastewaters of wastewater treatment plants (WWTP) is an environmental mandatory task to minimize or eliminate water pollution. The present work is divided in two main experimental stages. First, an SPE/HPLC-UV experimental methodology is optimized to detect and quantify 17β-Estradiol (E2) present in aqueous samples. The HPLC-UV operating conditions were selected by performing a screening between 10 different mobile phase compositions. A pure methanol composition was selected based in the lower retention time and the highest UV detector signal. The solid phase extraction optimization involves a three-level Box-Behnken experimental design with four factors (sample volume, sample pH, adsorbent drying time and solvent composition used for the washing step), combined with a response surface methodology. The validation of the optimized experimental methodology is done by the monitoring of estradiol in wastewater influent and effluent samples from Bragança Wastewater Treatment Plant in Portugal.
Main Authors:	Foureaux, Nathalia S.
Other Authors:	Carneiro, Eduardo V.; Ribeiro, António E.; Brito, Paulo; Queiroz, Ana
Subject:	Estradiol Wastewater Solid phase extraction High performance liquid chromatography
Year:	2021
Country:	Portugal
Document type:	conference output
Access type:	open access
Associated institution:	Universidade Politécnica de Bragança
Language:	English
Origin:	Biblioteca Digital da UPB

Description
Summary:	Nowadays, monitoring the concentration levels of estrogens in treated wastewaters of wastewater treatment plants (WWTP) is an environmental mandatory task to minimize or eliminate water pollution. The present work is divided in two main experimental stages. First, an SPE/HPLC-UV experimental methodology is optimized to detect and quantify 17β-Estradiol (E2) present in aqueous samples. The HPLC-UV operating conditions were selected by performing a screening between 10 different mobile phase compositions. A pure methanol composition was selected based in the lower retention time and the highest UV detector signal. The solid phase extraction optimization involves a three-level Box-Behnken experimental design with four factors (sample volume, sample pH, adsorbent drying time and solvent composition used for the washing step), combined with a response surface methodology. The validation of the optimized experimental methodology is done by the monitoring of estradiol in wastewater influent and effluent samples from Bragança Wastewater Treatment Plant in Portugal.

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