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Lipase assay in yarrowia lipolytica

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Bibliographic Details
Summary:Lipases (triacylglycerol acyl hydrolases, EC 3. 1.1.3) are key enzymes in fat metabolism produced by microorganisms, plants, and animals that catalyze the breakdown of triacylglycerols to free fatty adds and glycerol. In Y. lipolytica severaJ genes encoding lipases have been isoiated,LlP2 coding for a secreted lipase (Pignede et al. 1000) and LlPl and LIP3 encoding two lipase genes of the carboxylesrerase family (Choupina et al. 1999). Lipase expression was repressed by glucose and induced by fatty acids and olive oil.
Main Authors:Morín, M.
Other Authors:Gonzalez, Francisco J.; Choupina, Altino; Burguillo, Francisco J.; Dominguez, Ángel
Year:2003
Country:Portugal
Document type:book part
Access type:open access
Associated institution:Universidade Politécnica de Bragança
Language:English
Origin:Biblioteca Digital da UPB
Description
Summary:Lipases (triacylglycerol acyl hydrolases, EC 3. 1.1.3) are key enzymes in fat metabolism produced by microorganisms, plants, and animals that catalyze the breakdown of triacylglycerols to free fatty adds and glycerol. In Y. lipolytica severaJ genes encoding lipases have been isoiated,LlP2 coding for a secreted lipase (Pignede et al. 1000) and LlPl and LIP3 encoding two lipase genes of the carboxylesrerase family (Choupina et al. 1999). Lipase expression was repressed by glucose and induced by fatty acids and olive oil.

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