Publicação
Cell-free layer (CFL) analysis in a glass capillary: comparison between a manual and automatic method
| Resumo: | In this study, in vitro blood flowing through a 100 m glass capillary was studied. The images were captured using a confocal system and post-processed using Image J and MatLab. The aim of the present work, was to measure the trajectories of the cell-free layer (CFL) by using two different methods, i. e., a manual method (MM) and an automatic method (AM). For theMM we have used amanual tracking plugin (MTrackJ) from Image J to track labeled red blood cells (RBCs) flowing around the boundary of the RBCs core. For the AM we have used a MatLab scripts to measure automatically the CFL trajectories. The preliminary numerical results suggest that the CFL trajectories follow a polynomial function for both methods. |
|---|---|
| Autores principais: | Bento, David |
| Outros Autores: | Pinho, Diana; Pereira, Ana I.; Lima, Rui A. |
| Assunto: | Cell-free layer Automatic method Red blood cells Nonlinear optimization |
| Ano: | 2011 |
| País: | Portugal |
| Tipo de documento: | comunicação em conferência |
| Tipo de acesso: | acesso aberto |
| Instituição associada: | Universidade Politécnica de Bragança |
| Idioma: | inglês |
| Origem: | Biblioteca Digital da UPB |
| _version_ | 1877782334030217216 |
|---|---|
| author | Bento, David |
| author2 | Pinho, Diana Pereira, Ana I. Lima, Rui A. |
| author2_role | author author author |
| author_facet | Bento, David Pinho, Diana Pereira, Ana I. Lima, Rui A. |
| author_role | author |
| contributor_name_str_mv | Biblioteca Digital da UPB |
| country_str | PT |
| creators_json_txt | [{\"Person.name\":\"Bento, David\"},{\"Person.name\":\"Pinho, Diana\",\"Person.identifier.cienciaID\":\"5214-ED0C-35E8\",\"Person.identifier.orcid\":\"0000-0002-3884-6496\"},{\"Person.name\":\"Pereira, Ana I.\",\"Person.identifier.cienciaID\":\"0716-B7C2-93E4\",\"Person.identifier.orcid\":\"0000-0003-3803-2043\",\"Person.identifier.researcherID\":\"F-3168-2010\",\"Person.identifier.scopusAuthorID\":\"15071961600\"},{\"Person.name\":\"Lima, Rui A.\",\"Person.identifier.cienciaID\":\"EE12-C3FB-349D\",\"Person.identifier.orcid\":\"0000-0003-3428-637X\",\"Person.identifier.researcherID\":\"H-5157-2016\",\"Person.identifier.scopusAuthorID\":\"18437397800\"}] |
| datacite.contributors.contributor.contributorName.fl_str_mv | Biblioteca Digital da UPB |
| datacite.creators.creator.creatorName.fl_str_mv | Bento, David Pinho, Diana Pereira, Ana I. Lima, Rui A. |
| datacite.date.Accepted.fl_str_mv | 2011-01-01T00:00:00Z |
| datacite.date.available.fl_str_mv | 2014-10-30T10:57:17Z |
| datacite.date.embargoed.fl_str_mv | 2014-10-30T10:57:17Z |
| datacite.rights.fl_str_mv | http://purl.org/coar/access_right/c_abf2 |
| datacite.subjects.subject.fl_str_mv | Cell-free layer Automatic method Red blood cells Nonlinear optimization |
| datacite.titles.title.fl_str_mv | Cell-free layer (CFL) analysis in a glass capillary: comparison between a manual and automatic method |
| dc.contributor.none.fl_str_mv | Biblioteca Digital da UPB |
| dc.creator.none.fl_str_mv | Bento, David Pinho, Diana Pereira, Ana I. Lima, Rui A. |
| dc.date.Accepted.fl_str_mv | 2011-01-01T00:00:00Z |
| dc.date.available.fl_str_mv | 2014-10-30T10:57:17Z |
| dc.date.embargoed.fl_str_mv | 2014-10-30T10:57:17Z |
| dc.format.none.fl_str_mv | application/pdf |
| dc.identifier.none.fl_str_mv | http://hdl.handle.net/10198/11216 |
| dc.language.none.fl_str_mv | eng |
| dc.publisher.none.fl_str_mv | AIP Conference Proceedings |
| dc.rights.none.fl_str_mv | http://purl.org/coar/access_right/c_abf2 |
| dc.subject.none.fl_str_mv | Cell-free layer Automatic method Red blood cells Nonlinear optimization |
| dc.title.fl_str_mv | Cell-free layer (CFL) analysis in a glass capillary: comparison between a manual and automatic method |
| dc.type.none.fl_str_mv | http://purl.org/coar/resource_type/c_5794 |
| description | In this study, in vitro blood flowing through a 100 m glass capillary was studied. The images were captured using a confocal system and post-processed using Image J and MatLab. The aim of the present work, was to measure the trajectories of the cell-free layer (CFL) by using two different methods, i. e., a manual method (MM) and an automatic method (AM). For theMM we have used amanual tracking plugin (MTrackJ) from Image J to track labeled red blood cells (RBCs) flowing around the boundary of the RBCs core. For the AM we have used a MatLab scripts to measure automatically the CFL trajectories. The preliminary numerical results suggest that the CFL trajectories follow a polynomial function for both methods. |
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| eu_rights_str_mv | openAccess |
| format | conferencePaper |
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| funding.funder.alternateName_str_mv | FCT FCT FCT FCT |
| funding.funder.identifier_str_mv | http://doi.org/10.13039/501100001871 http://doi.org/10.13039/501100001871 http://doi.org/10.13039/501100001871 http://doi.org/10.13039/501100001871 |
| funding.funder.name_str_mv | Fundação para a Ciência e a Tecnologia Fundação para a Ciência e a Tecnologia Fundação para a Ciência e a Tecnologia Fundação para a Ciência e a Tecnologia |
| funding.identifier_str_mv | PTDC/EME-MFE/099109/2008 PTDC/SAU-BEB/108728/2008 PTDC/SAU-BEB/105650/2008 PTDC/SAU-ENB/116929/2010 |
| funding.name_str_mv | 5876-PPCDTI 5876-PPCDTI 5876-PPCDTI 5876-PPCDTI |
| funding_str_mv | PTDC/EME-MFE/099109/2008 info:eu-repo/grantAgreement/FCT/5876-PPCDTI/PTDC%2FEME-MFE%2F099109%2F2008/PT PTDC/SAU-BEB/108728/2008 info:eu-repo/grantAgreement/FCT/5876-PPCDTI/PTDC%2FSAU-BEB%2F108728%2F2008/PT PTDC/SAU-BEB/105650/2008 info:eu-repo/grantAgreement/FCT/5876-PPCDTI/PTDC%2FSAU-BEB%2F105650%2F2008/PT PTDC/SAU-ENB/116929/2010 info:eu-repo/grantAgreement/FCT/5876-PPCDTI/PTDC%2FSAU-ENB%2F116929%2F2010/PT |
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| identifier.url.fl_str_mv | http://hdl.handle.net/10198/11216 |
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| person_str_mv | Bento, David Pinho, Diana Pinho, Diana https://www.ciencia-id.pt/5214-ED0C-35E8 5214-ED0C-35E8 http://orcid.org/0000-0002-3884-6496 0000-0002-3884-6496 Pereira, Ana I. Pereira, Ana I. https://www.ciencia-id.pt/0716-B7C2-93E4 0716-B7C2-93E4 http://orcid.org/0000-0003-3803-2043 0000-0003-3803-2043 Lima, Rui A. Lima, Rui A. https://www.ciencia-id.pt/EE12-C3FB-349D EE12-C3FB-349D http://orcid.org/0000-0003-3428-637X 0000-0003-3428-637X |
| publishDate | 2011 |
| publisher.none.fl_str_mv | AIP Conference Proceedings |
| repo_facet_str | urn:repositoryAcronym:ipb{{{_:::_}}}Biblioteca Digital da UPB |
| reponame_str | Biblioteca Digital da UPB |
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| spelling | engAIP Conference ProceedingsporIn this study, in vitro blood flowing through a 100 m glass capillary was studied. The images were captured using a confocal system and post-processed using Image J and MatLab. The aim of the present work, was to measure the trajectories of the cell-free layer (CFL) by using two different methods, i. e., a manual method (MM) and an automatic method (AM). For theMM we have used amanual tracking plugin (MTrackJ) from Image J to track labeled red blood cells (RBCs) flowing around the boundary of the RBCs core. For the AM we have used a MatLab scripts to measure automatically the CFL trajectories. The preliminary numerical results suggest that the CFL trajectories follow a polynomial function for both methods.application/pdfporCell-free layer (CFL) analysis in a glass capillary: comparison between a manual and automatic methodBento, DavidPersonalPinho, DianaDSpacehttp://dspace.org/items/02bf2dd9-33ed-4d87-b61d-ca39c51f8451DSpacehttp://dspace.org/items/02bf2dd9-33ed-4d87-b61d-ca39c51f8451PinhoDianaCiência IDhttps://www.ciencia-id.pt5214-ED0C-35E8ORCIDhttp://orcid.org0000-0002-3884-6496PersonalPereira, Ana I.DSpacehttp://dspace.org/items/e9981d62-2a2b-4fef-b75e-c2a14b0e7846DSpacehttp://dspace.org/items/e9981d62-2a2b-4fef-b75e-c2a14b0e7846PereiraAna I.Ciência IDhttps://www.ciencia-id.pt0716-B7C2-93E4ORCIDhttp://orcid.org0000-0003-3803-2043Researcher IDhttps://www.researcherid.comF-3168-2010Scopus Author IDhttps://www.scopus.com15071961600PersonalLima, Rui A.DSpacehttp://dspace.org/items/7b50c499-8095-4f4f-8b1b-fa7388e4ff62DSpacehttp://dspace.org/items/7b50c499-8095-4f4f-8b1b-fa7388e4ff62LimaRui A.Ciência IDhttps://www.ciencia-id.ptEE12-C3FB-349DORCIDhttp://orcid.org0000-0003-3428-637XResearcher IDhttps://www.researcherid.comH-5157-2016Scopus Author IDhttps://www.scopus.com18437397800HostingInstitutionOrganizationalBiblioteca Digital da UPBe-mailmailto:dspace@unipb.ptdspace@unipb.pt2014-10-30T10:57:17Z20112011-01-01T00:00:00ZHandlehttp://hdl.handle.net/10198/11216http://purl.org/coar/access_right/c_abf2open accessCell-free layerAutomatic methodRed blood cellsNonlinear optimization355476 bytesFundação para a Ciência e a TecnologiaANEURYSM - Hemodynamic Study in Cerebral Aneurysmsinfo:eu-repo/grantAgreement/FCT/5876-PPCDTI/PTDC%2FEME-MFE%2F099109%2F2008/PTPTDC/EME-MFE/099109/20085876-PPCDTICrossref Funder IDhttp://doi.org/10.13039/501100001871Fundação para a Ciência e a TecnologiaHemo-Networks - Experimental and computational investigations of the factors influencing blood flow in microvascular networks.info:eu-repo/grantAgreement/FCT/5876-PPCDTI/PTDC%2FSAU-BEB%2F108728%2F2008/PTPTDC/SAU-BEB/108728/20085876-PPCDTICrossref Funder IDhttp://doi.org/10.13039/501100001871Fundação para a Ciência e a TecnologiaCSD-Chip - Desenvolvimento de um biochip para a separação e deformação de células sanguíneasinfo:eu-repo/grantAgreement/FCT/5876-PPCDTI/PTDC%2FSAU-BEB%2F105650%2F2008/PTPTDC/SAU-BEB/105650/20085876-PPCDTICrossref Funder IDhttp://doi.org/10.13039/501100001871Fundação para a Ciência e a TecnologiaBIOMIMETIC - A biomimetic microdevice for the diagnosis of erythrocytes diseases based on their deformabilityinfo:eu-repo/grantAgreement/FCT/5876-PPCDTI/PTDC%2FSAU-ENB%2F116929%2F2010/PTPTDC/SAU-ENB/116929/20105876-PPCDTICrossref Funder IDhttp://doi.org/10.13039/501100001871other research producthttp://purl.org/coar/resource_type/c_5794conference paperhttp://purl.org/coar/access_right/c_abf2application/pdffulltexthttps://bibliotecadigital.unipb.pt/bitstreams/500095e1-71b7-40c3-9749-42582496c6ac/downloadInternational Conference on Numerical Analysis and Applied Mathematics786789Greece |
| spellingShingle | Cell-free layer (CFL) analysis in a glass capillary: comparison between a manual and automatic method Bento, David Cell-free layer Automatic method Red blood cells Nonlinear optimization |
| status | SINGLETON |
| subject.fl_str_mv | Cell-free layer Automatic method Red blood cells Nonlinear optimization |
| title | Cell-free layer (CFL) analysis in a glass capillary: comparison between a manual and automatic method |
| title_full | Cell-free layer (CFL) analysis in a glass capillary: comparison between a manual and automatic method |
| title_fullStr | Cell-free layer (CFL) analysis in a glass capillary: comparison between a manual and automatic method |
| title_full_unstemmed | Cell-free layer (CFL) analysis in a glass capillary: comparison between a manual and automatic method |
| title_short | Cell-free layer (CFL) analysis in a glass capillary: comparison between a manual and automatic method |
| title_sort | Cell-free layer (CFL) analysis in a glass capillary: comparison between a manual and automatic method |
| topic | Cell-free layer Automatic method Red blood cells Nonlinear optimization |
| topic_facet | Cell-free layer Automatic method Red blood cells Nonlinear optimization |
| url | http://hdl.handle.net/10198/11216 |
| visible | 1 |