Publicação

Cell-free layer (CFL) analysis in a glass capillary: comparison between a manual and automatic method

Detalhes bibliográficos
Resumo:	In this study, in vitro blood flowing through a 100 m glass capillary was studied. The images were captured using a confocal system and post-processed using Image J and MatLab. The aim of the present work, was to measure the trajectories of the cell-free layer (CFL) by using two different methods, i. e., a manual method (MM) and an automatic method (AM). For theMM we have used amanual tracking plugin (MTrackJ) from Image J to track labeled red blood cells (RBCs) flowing around the boundary of the RBCs core. For the AM we have used a MatLab scripts to measure automatically the CFL trajectories. The preliminary numerical results suggest that the CFL trajectories follow a polynomial function for both methods.
Autores principais:	Bento, David
Outros Autores:	Pinho, Diana; Pereira, Ana I.; Lima, Rui A.
Assunto:	Cell-free layer Automatic method Red blood cells Nonlinear optimization
Ano:	2011
País:	Portugal
Tipo de documento:	comunicação em conferência
Tipo de acesso:	acesso aberto
Instituição associada:	Universidade Politécnica de Bragança
Idioma:	inglês
Origem:	Biblioteca Digital da UPB

Descrição
Resumo:	In this study, in vitro blood flowing through a 100 m glass capillary was studied. The images were captured using a confocal system and post-processed using Image J and MatLab. The aim of the present work, was to measure the trajectories of the cell-free layer (CFL) by using two different methods, i. e., a manual method (MM) and an automatic method (AM). For theMM we have used amanual tracking plugin (MTrackJ) from Image J to track labeled red blood cells (RBCs) flowing around the boundary of the RBCs core. For the AM we have used a MatLab scripts to measure automatically the CFL trajectories. The preliminary numerical results suggest that the CFL trajectories follow a polynomial function for both methods.

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