Publicação
THERAPEUTIC EFFECTS OF NOVEL DRESSING COATED LL37-AuNP FOR THE ENHANCED WOUND HEALING
| Resumo: | Despite an increasing awareness of the pathophysiologic events that takes place in the development of wounds of various underlying etiologies, impaired wound healing and its medical complications remain one of the most prevalent and economically burdensome healthcare issues in the world. Approximately 6.5 US million patients are affected by chronic wounds and more than US$25 billion are spent annually for the treatment of this condition. Chronic wounds have many psychosocial consequences, ranging from social isolation to mental malaise. In addition, impaired wound healing and its medical complications remains one of the most prevalent and economically burdensome healthcare issues in the world, due to an increase in aging population and a rise in diabetes and obesity incidence around the world, impaired wound healing will continue to increase. Given this, research into tissue regeneration in chronic wound repair is vital, as well as the development of new therapeutics is absolutely necessary and important to satisfy the unmet clinical need.In this thesis, we studied the therapeutic effects of LL37 conjugated gold nanoparticles coated polyurethane (LL37-AuNP-PU) dressing, which is a novel technology developed by our lab. We performed biocompatibility assays and showed that the viability of the human keratinocytes cells (HaCat cells) was not compromised, indicating that LL37-AuNP-PU dressing is not toxic to the cells. In addition, we also showed that this treatment induces wound closure trough expression of more keratinocytes in the proliferative edges of the wound, as well as in the wound gap. Furthermore, LL37-AuNP-PU dressing regulates the activation of macrophages and improves the progression of the inflammatory stages of wound healing, which makes this environment more prone to the beginning of proliferative and remodeling stages of wound healing.Overall, the results presented in this thesis demonstrated that LL-37-AuNP-PU dressings could potentially be used for the treatment of chronic wounds, diabetic wounds and burns, while preventing bacterial infection. |
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| Autores principais: | Monteiro, Denise Irene Marta |
| Assunto: | Patch LL37-AuNP-PU Feridas crónicas Feridas crónicas Queratinócitos Tratamento LL37-AuNP-PU dressing Chronic Wounds Macrophage regulation Keratinocytes Treatment |
| Ano: | 2019 |
| País: | Portugal |
| Tipo de documento: | dissertação de mestrado |
| Tipo de acesso: | acesso aberto |
| Instituição associada: | Universidade de Coimbra |
| Idioma: | inglês |
| Origem: | Estudo Geral - Universidade de Coimbra |
| Resumo: | Despite an increasing awareness of the pathophysiologic events that takes place in the development of wounds of various underlying etiologies, impaired wound healing and its medical complications remain one of the most prevalent and economically burdensome healthcare issues in the world. Approximately 6.5 US million patients are affected by chronic wounds and more than US$25 billion are spent annually for the treatment of this condition. Chronic wounds have many psychosocial consequences, ranging from social isolation to mental malaise. In addition, impaired wound healing and its medical complications remains one of the most prevalent and economically burdensome healthcare issues in the world, due to an increase in aging population and a rise in diabetes and obesity incidence around the world, impaired wound healing will continue to increase. Given this, research into tissue regeneration in chronic wound repair is vital, as well as the development of new therapeutics is absolutely necessary and important to satisfy the unmet clinical need.In this thesis, we studied the therapeutic effects of LL37 conjugated gold nanoparticles coated polyurethane (LL37-AuNP-PU) dressing, which is a novel technology developed by our lab. We performed biocompatibility assays and showed that the viability of the human keratinocytes cells (HaCat cells) was not compromised, indicating that LL37-AuNP-PU dressing is not toxic to the cells. In addition, we also showed that this treatment induces wound closure trough expression of more keratinocytes in the proliferative edges of the wound, as well as in the wound gap. Furthermore, LL37-AuNP-PU dressing regulates the activation of macrophages and improves the progression of the inflammatory stages of wound healing, which makes this environment more prone to the beginning of proliferative and remodeling stages of wound healing.Overall, the results presented in this thesis demonstrated that LL-37-AuNP-PU dressings could potentially be used for the treatment of chronic wounds, diabetic wounds and burns, while preventing bacterial infection. |
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