Publicação
Deteção e Quantificação de fármacos em ovas de peixe por UHPLC-ToF-MS
| Resumo: | The increase in the world's population and life expectancy has also led to a global increase in the consumption of drugs, which are now recognised as emerging pollutants. Their potential adverse effects are a cause for concern because there is still a lack of effective methods for the treatment and disposal of waste and possible pharmaceutical contaminants in soil and water, thus bringing consequences for the environment, such as alterations in the life cycle of animals and microorganisms. The main origin of drugs in the environment is mainly due to their excretion, ineffective removal in sewage treatment plants and effluents from the pharmaceutical industry and agriculture, among other sources, leading to contamination in the aquatic environment. Due to the continuous introduction of drugs into ecosystems, aquatic organisms are often exposed throughout their life cycle, promoting an environmental impact. Although drugs are detected in low concentrations, they can cause toxicity because they are developed to promote a biological effect.The main aim of this study is to identify and quantify drugs by validanting a ultra-high performance liquid chromatography linked to a mass spectrometer with time-of-flight analyser (UHPLC-TOF-MS) methodology for fish roe. After validation, 45 samples of fish roe from different species namely, croaker (Argyrosomus regius), letter (Arnoglossus laterna), redhead (Chelidonichthys lucerna), hake (Merluccius merluccius), sand sole (Pegusa lascaris), turbot (Scophthalmus rhombus), white sole (Solea senegalensis) and pout (Trisopterus luscus) were analysed to investigate whether there is contamination and how this poses a risk to consumer health. Of the 45 samples, contamination was detected in 7 samples of two different species, in pout (Trisopterus luscus) and hake (Merluccius merluccius). Two drug residues were identified, clozapine in 6 samples of pout with concentrations ranging between 22.1µg/kg and 35.4 µg/kg and gabapentin detected in one sample of hake in which the concentration obtained was 11.9 µg/kg. These results show that it is relevant to have drug residue monitoring and control programmes in place, as confirmation of drug contamination in fish roe can lead to bioaccumulation, raising additional food safety concerns. |
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| Autores principais: | Matos, Laura de Almeida |
| Assunto: | Drugs Fish roe Food safety Environmental impact UHPLC-ToF-MS Fármacos Ovas de peixe Segurança Alimentar Impacto Ambiental UHPLC-ToF-MS |
| Ano: | 2024 |
| País: | Portugal |
| Tipo de documento: | dissertação de mestrado |
| Tipo de acesso: | acesso embargado |
| Instituição associada: | Universidade de Coimbra |
| Idioma: | português |
| Origem: | Estudo Geral - Universidade de Coimbra |
| Resumo: | The increase in the world's population and life expectancy has also led to a global increase in the consumption of drugs, which are now recognised as emerging pollutants. Their potential adverse effects are a cause for concern because there is still a lack of effective methods for the treatment and disposal of waste and possible pharmaceutical contaminants in soil and water, thus bringing consequences for the environment, such as alterations in the life cycle of animals and microorganisms. The main origin of drugs in the environment is mainly due to their excretion, ineffective removal in sewage treatment plants and effluents from the pharmaceutical industry and agriculture, among other sources, leading to contamination in the aquatic environment. Due to the continuous introduction of drugs into ecosystems, aquatic organisms are often exposed throughout their life cycle, promoting an environmental impact. Although drugs are detected in low concentrations, they can cause toxicity because they are developed to promote a biological effect.The main aim of this study is to identify and quantify drugs by validanting a ultra-high performance liquid chromatography linked to a mass spectrometer with time-of-flight analyser (UHPLC-TOF-MS) methodology for fish roe. After validation, 45 samples of fish roe from different species namely, croaker (Argyrosomus regius), letter (Arnoglossus laterna), redhead (Chelidonichthys lucerna), hake (Merluccius merluccius), sand sole (Pegusa lascaris), turbot (Scophthalmus rhombus), white sole (Solea senegalensis) and pout (Trisopterus luscus) were analysed to investigate whether there is contamination and how this poses a risk to consumer health. Of the 45 samples, contamination was detected in 7 samples of two different species, in pout (Trisopterus luscus) and hake (Merluccius merluccius). Two drug residues were identified, clozapine in 6 samples of pout with concentrations ranging between 22.1µg/kg and 35.4 µg/kg and gabapentin detected in one sample of hake in which the concentration obtained was 11.9 µg/kg. These results show that it is relevant to have drug residue monitoring and control programmes in place, as confirmation of drug contamination in fish roe can lead to bioaccumulation, raising additional food safety concerns. |
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