Publicação
Sequential injection system for the enzymatic determination of ethanol in wine
| Resumo: | A sequential injection system was developed for the enzymatic determination of ethanol in wine. The spectrophotometric determination is based on the enzymatic reaction catalyzed by alcohol dehydrogenase in the presence of NAD+. The system was applied to the determination of ethanol in a range of 0.008-0.024% (v/v) with good repeatability; RSDn)10 < 2.3%. The results obtained with the developed system showed good agreement with those obtained by using the reference method. The determination rate was 25 h-1; 1 ímol of NAD+, 1.1 units of enzyme, and 50 íL of sample were consumed per determination; and the waste produced was 2.2 mL per assay. |
|---|---|
| Autores principais: | Páscoa, Ricardo N. M. J. |
| Outros Autores: | Vidigal, Susana S. M. P.; Tóth, Ildikó Varga; Rangel, António O. S. S. |
| Assunto: | Sequential injection analysis Alcohol dehydrogenase Ethanol Wine Spectrophotometry |
| Ano: | 2006 |
| País: | Portugal |
| Tipo de documento: | artigo |
| Tipo de acesso: | acesso restrito |
| Instituição associada: | Universidade Católica Portuguesa |
| Idioma: | inglês |
| Origem: | Veritati - Repositório Institucional da Universidade Católica Portuguesa |
| _version_ | 1877783976401174528 |
|---|---|
| author | Páscoa, Ricardo N. M. J. |
| author2 | Vidigal, Susana S. M. P. Tóth, Ildikó Varga Rangel, António O. S. S. |
| author2_role | author author author |
| author_facet | Páscoa, Ricardo N. M. J. Vidigal, Susana S. M. P. Tóth, Ildikó Varga Rangel, António O. S. S. |
| author_role | author |
| contributor_name_str_mv | Veritati |
| country_str | PT |
| creators_json_txt | [{\"Person.name\":\"Páscoa, Ricardo N. M. J.\",\"Person.identifier.cienciaID\":\"BA14-0549-AA0A\",\"Person.identifier.orcid\":\"0000-0003-2073-7643\",\"Person.identifier.researcherID\":\"D-4791-2013\",\"Person.identifier.scopusAuthorID\":\"11738933900\"},{\"Person.name\":\"Vidigal, Susana S. M. P.\",\"Person.identifier.cienciaID\":\"2919-B77C-11C0\",\"Person.identifier.orcid\":\"0000-0002-0213-063X\",\"Person.identifier.researcherID\":\"M-4661-2013\",\"Person.identifier.scopusAuthorID\":\"11739488200\"},{\"Person.name\":\"Tóth, Ildikó Varga\",\"Person.identifier.orcid\":\"0000-0002-7342-1063\",\"Person.identifier.researcherID\":\"G-1733-2010\",\"Person.identifier.scopusAuthorID\":\"35512332800\"},{\"Person.name\":\"Rangel, António O. S. S.\",\"Person.identifier.cienciaID\":\"D61D-7CFB-178A\",\"Person.identifier.orcid\":\"0000-0002-6486-8947\",\"Person.identifier.scopusAuthorID\":\"7004827387\"}] |
| datacite.contributors.contributor.contributorName.fl_str_mv | Veritati |
| datacite.creators.creator.creatorName.fl_str_mv | Páscoa, Ricardo N. M. J. Vidigal, Susana S. M. P. Tóth, Ildikó Varga Rangel, António O. S. S. |
| datacite.date.Accepted.fl_str_mv | 2006-01-01T00:00:00Z |
| datacite.date.available.fl_str_mv | 2011-10-22T11:01:54Z |
| datacite.date.embargoed.fl_str_mv | 2011-10-22T11:01:54Z |
| datacite.rights.fl_str_mv | http://purl.org/coar/access_right/c_16ec |
| datacite.subjects.subject.fl_str_mv | Sequential injection analysis Alcohol dehydrogenase Ethanol Wine Spectrophotometry |
| datacite.titles.title.fl_str_mv | Sequential injection system for the enzymatic determination of ethanol in wine |
| dc.contributor.none.fl_str_mv | Veritati |
| dc.creator.none.fl_str_mv | Páscoa, Ricardo N. M. J. Vidigal, Susana S. M. P. Tóth, Ildikó Varga Rangel, António O. S. S. |
| dc.date.Accepted.fl_str_mv | 2006-01-01T00:00:00Z |
| dc.date.available.fl_str_mv | 2011-10-22T11:01:54Z |
| dc.date.embargoed.fl_str_mv | 2011-10-22T11:01:54Z |
| dc.format.none.fl_str_mv | application/pdf |
| dc.identifier.none.fl_str_mv | http://hdl.handle.net/10400.14/6786 |
| dc.language.none.fl_str_mv | eng |
| dc.publisher.none.fl_str_mv | American Chemical Society |
| dc.rights.none.fl_str_mv | http://purl.org/coar/access_right/c_16ec |
| dc.subject.none.fl_str_mv | Sequential injection analysis Alcohol dehydrogenase Ethanol Wine Spectrophotometry |
| dc.title.fl_str_mv | Sequential injection system for the enzymatic determination of ethanol in wine |
| dc.type.none.fl_str_mv | http://purl.org/coar/resource_type/c_6501 |
| description | A sequential injection system was developed for the enzymatic determination of ethanol in wine. The spectrophotometric determination is based on the enzymatic reaction catalyzed by alcohol dehydrogenase in the presence of NAD+. The system was applied to the determination of ethanol in a range of 0.008-0.024% (v/v) with good repeatability; RSDn)10 < 2.3%. The results obtained with the developed system showed good agreement with those obtained by using the reference method. The determination rate was 25 h-1; 1 ímol of NAD+, 1.1 units of enzyme, and 50 íL of sample were consumed per determination; and the waste produced was 2.2 mL per assay. |
| dirty | 0 |
| eu_rights_str_mv | restrictedAccess |
| format | article |
| fulltext.url.fl_str_mv | https://repositorio.ucp.pt/bitstreams/fd93ad7d-c1fd-4fd2-9504-965a2dfe91dc/download |
| id | veritati_badcb3ca19a17c2ce85f484bf98b71fd |
| identifier.url.fl_str_mv | http://hdl.handle.net/10400.14/6786 |
| inst_facet_str | urn:organizationAcronym:ucp{{{_:::_}}}Universidade Católica Portuguesa |
| instacron_str | ucp |
| institution | Universidade Católica Portuguesa |
| instname_str | Universidade Católica Portuguesa |
| language | eng |
| network_acronym_str | veritati |
| network_name_str | Veritati - Repositório Institucional da Universidade Católica Portuguesa |
| oai_identifier_str | oai:repositorio.ucp.pt:10400.14/6786 |
| organization_str_mv | urn:organizationAcronym:ucp |
| person_str_mv | Páscoa, Ricardo N. M. J. Páscoa, Ricardo N. M. J. https://www.ciencia-id.pt/BA14-0549-AA0A BA14-0549-AA0A http://orcid.org/0000-0003-2073-7643 0000-0003-2073-7643 Vidigal, Susana S. M. P. Vidigal, Susana S. M. P. https://www.ciencia-id.pt/2919-B77C-11C0 2919-B77C-11C0 http://orcid.org/0000-0002-0213-063X 0000-0002-0213-063X Tóth, Ildikó Varga Tóth, Ildikó Varga http://orcid.org/0000-0002-7342-1063 0000-0002-7342-1063 Rangel, António O. S. S. Rangel, António O. S. S. https://www.ciencia-id.pt/D61D-7CFB-178A D61D-7CFB-178A http://orcid.org/0000-0002-6486-8947 0000-0002-6486-8947 |
| publishDate | 2006 |
| publisher.none.fl_str_mv | American Chemical Society |
| repo_facet_str | urn:repositoryAcronym:veritati{{{_:::_}}}Veritati - Repositório Institucional da Universidade Católica Portuguesa |
| reponame_str | Veritati - Repositório Institucional da Universidade Católica Portuguesa |
| repository_id_str | urn:repositoryAcronym:veritati |
| service_str_mv | urn:repositoryAcronym:veritati |
| spelling | engAmerican Chemical SocietyporA sequential injection system was developed for the enzymatic determination of ethanol in wine. The spectrophotometric determination is based on the enzymatic reaction catalyzed by alcohol dehydrogenase in the presence of NAD+. The system was applied to the determination of ethanol in a range of 0.008-0.024% (v/v) with good repeatability; RSDn)10 < 2.3%. The results obtained with the developed system showed good agreement with those obtained by using the reference method. The determination rate was 25 h-1; 1 ímol of NAD+, 1.1 units of enzyme, and 50 íL of sample were consumed per determination; and the waste produced was 2.2 mL per assay.application/pdfporSequential injection system for the enzymatic determination of ethanol in winePersonalPáscoa, Ricardo N. M. J.DSpacehttp://dspace.org/items/2140ed4c-4229-4ff5-9940-c7b476f09741DSpacehttp://dspace.org/items/2140ed4c-4229-4ff5-9940-c7b476f09741PáscoaRicardoCiência IDhttps://www.ciencia-id.ptBA14-0549-AA0AORCIDhttp://orcid.org0000-0003-2073-7643Researcher IDhttps://www.researcherid.comD-4791-2013Scopus Author IDhttps://www.scopus.com11738933900PersonalVidigal, Susana S. M. P.DSpacehttp://dspace.org/items/59453cc2-ee50-4a1d-bbfc-924ca6417004DSpacehttp://dspace.org/items/59453cc2-ee50-4a1d-bbfc-924ca6417004VidigalSusanaCiência IDhttps://www.ciencia-id.pt2919-B77C-11C0ORCIDhttp://orcid.org0000-0002-0213-063XResearcher IDhttps://www.researcherid.comM-4661-2013Scopus Author IDhttps://www.scopus.com11739488200PersonalTóth, Ildikó VargaDSpacehttp://dspace.org/items/7889563f-f3fc-4057-a97c-b51425b4dc46DSpacehttp://dspace.org/items/7889563f-f3fc-4057-a97c-b51425b4dc46TóthIldikóORCIDhttp://orcid.org0000-0002-7342-1063Researcher IDhttps://www.researcherid.comG-1733-2010Scopus Author IDhttps://www.scopus.com35512332800PersonalRangel, António O. S. S.DSpacehttp://dspace.org/items/467be022-fd9c-4549-aa48-48ebcc1098ebDSpacehttp://dspace.org/items/467be022-fd9c-4549-aa48-48ebcc1098ebSantos Silva RangelAntónio OsmaroCiência IDhttps://www.ciencia-id.ptD61D-7CFB-178AORCIDhttp://orcid.org0000-0002-6486-8947Scopus Author IDhttps://www.scopus.com7004827387HostingInstitutionOrganizationalVeritatie-mailmailto:repositorio@ucp.ptrepositorio@ucp.ptDOIIsPartOf10.1021/jf051787l2011-10-22T11:01:54Z20062006-01-01T00:00:00ZHandlehttp://hdl.handle.net/10400.14/6786http://purl.org/coar/access_right/c_16ecrestricted accessSequential injection analysisAlcohol dehydrogenaseEthanolWineSpectrophotometry83173 bytesliteraturehttp://purl.org/coar/resource_type/c_6501journal articlehttp://purl.org/coar/access_right/c_16ecapplication/pdffulltexthttps://repositorio.ucp.pt/bitstreams/fd93ad7d-c1fd-4fd2-9504-965a2dfe91dc/download |
| spellingShingle | Sequential injection system for the enzymatic determination of ethanol in wine Páscoa, Ricardo N. M. J. Sequential injection analysis Alcohol dehydrogenase Ethanol Wine Spectrophotometry |
| status | SINGLETON |
| subject.fl_str_mv | Sequential injection analysis Alcohol dehydrogenase Ethanol Wine Spectrophotometry |
| title | Sequential injection system for the enzymatic determination of ethanol in wine |
| title_full | Sequential injection system for the enzymatic determination of ethanol in wine |
| title_fullStr | Sequential injection system for the enzymatic determination of ethanol in wine |
| title_full_unstemmed | Sequential injection system for the enzymatic determination of ethanol in wine |
| title_short | Sequential injection system for the enzymatic determination of ethanol in wine |
| title_sort | Sequential injection system for the enzymatic determination of ethanol in wine |
| topic | Sequential injection analysis Alcohol dehydrogenase Ethanol Wine Spectrophotometry |
| topic_facet | Sequential injection analysis Alcohol dehydrogenase Ethanol Wine Spectrophotometry |
| url | http://hdl.handle.net/10400.14/6786 |
| visible | 1 |
Atividades financiadas
Não foram encontrados projetos financiados.