Publicação
Bacterial degradation of moxifloxacin in the presence of acetate as a bulk substrate
| Resumo: | Fluoroquinolones constitute a group of emerging pollutants and their occurrence in different environmental compartments is becoming object of increasing public concern due to their ecotoxicological effects and the potential to develop resistant bacteria. This study aimed to investigate the biodegradation of moxifloxacin (MOX), for which studies in the literature are very scarce. An activated sludge (AS) consortium and three bacterial strains able to degrade fluoroaromatic compounds e strains F11, FP1 and S2 e were tested. Biodegradation studies were conducted using acetate as a bulk carbon source. Strain F11 showed the highest biodegradation capacity, being able to completely consume and dehalogenate 7.5 mM of the target antibiotic when daily co-supplemented with acetate present as a readily degradable organic substrate in wastewaters. MOX could be used by strain F11 as a sole nitrogen source but the presence of an external nitrogen source in the culture medium was essential for complete biodegradation. Strain F11 was capable of completely consuming MOX in a range between 2 and 11 mM, although stoichiometric fluoride release was not obtained for the highest tested concentration. The antibacterial activity of residual MOX and of the metabolic products potentially resultant from the biodegradation process was investigated by agar diffusion tests, demonstrating that MOX biodegradation is associated with the elimination of the antibacterial properties of the target antibiotic and of the produced metabolites, which is an important result, as the activity of antibiotics and/or their metabolites in the environment, even at low levels, may lead to the development of resistant bacterial strains. Overall, the results obtained in this study suggest that strain F11 is a promising microorganism for the treatment of waters contaminated with MOX, where it could be used for bioaugmentation/bioremediation purposes. To the best of our knowledge, this is the first study reporting complete removal and dehalogenation of MOX by a single microorganism. |
|---|---|
| Autores principais: | Carvalho, M. F. |
| Outros Autores: | Maia, A. S.; Tiritan, M. E.; Castro, P. M. L. |
| Assunto: | Moxifloxacin Fluoroquinolones Biodegradation Acetate Antimicrobial activity |
| Ano: | 2016 |
| País: | Portugal |
| Tipo de documento: | artigo |
| Tipo de acesso: | acesso restrito |
| Instituição associada: | Universidade Católica Portuguesa |
| Idioma: | inglês |
| Origem: | Veritati - Repositório Institucional da Universidade Católica Portuguesa |
| _version_ | 1877783833472925696 |
|---|---|
| author | Carvalho, M. F. |
| author2 | Maia, A. S. Tiritan, M. E. Castro, P. M. L. |
| author2_role | author author author |
| author_facet | Carvalho, M. F. Maia, A. S. Tiritan, M. E. Castro, P. M. L. |
| author_role | author |
| contributor_name_str_mv | Veritati |
| country_str | PT |
| creators_json_txt | [{\"Person.name\":\"Carvalho, M. F.\",\"Person.identifier.cienciaID\":\"6913-1464-155B\",\"Person.identifier.orcid\":\"0000-0002-7181-0540\",\"Person.identifier.scopusAuthorID\":\"14038677200\"},{\"Person.name\":\"Maia, A. S.\",\"Person.identifier.cienciaID\":\"6A15-F0A3-AEF2\",\"Person.identifier.orcid\":\"0000-0002-4167-383X\",\"Person.identifier.scopusAuthorID\":\"55332349300\"},{\"Person.name\":\"Tiritan, M. E.\",\"Person.identifier.cienciaID\":\"D915-BFC0-FB3A\",\"Person.identifier.orcid\":\"0000-0003-3320-730X\",\"Person.identifier.researcherID\":\"K-9492-2014\",\"Person.identifier.scopusAuthorID\":\"6602890981\"},{\"Person.name\":\"Castro, P. M. L.\",\"Person.identifier.cienciaID\":\"7C1F-6C72-354A\",\"Person.identifier.orcid\":\"0000-0001-8841-6606\",\"Person.identifier.researcherID\":\"M-8241-2013\",\"Person.identifier.scopusAuthorID\":\"7102781782\"}] |
| datacite.contributors.contributor.contributorName.fl_str_mv | Veritati |
| datacite.creators.creator.creatorName.fl_str_mv | Carvalho, M. F. Maia, A. S. Tiritan, M. E. Castro, P. M. L. |
| datacite.date.Accepted.fl_str_mv | 2016-01-01T00:00:00Z |
| datacite.date.available.fl_str_mv | 2017-10-13T08:02:31Z |
| datacite.date.embargoed.fl_str_mv | 2017-10-13T08:02:31Z |
| datacite.rights.fl_str_mv | http://purl.org/coar/access_right/c_16ec |
| datacite.subjects.subject.fl_str_mv | Moxifloxacin Fluoroquinolones Biodegradation Acetate Antimicrobial activity |
| datacite.titles.title.fl_str_mv | Bacterial degradation of moxifloxacin in the presence of acetate as a bulk substrate |
| dc.contributor.none.fl_str_mv | Veritati |
| dc.creator.none.fl_str_mv | Carvalho, M. F. Maia, A. S. Tiritan, M. E. Castro, P. M. L. |
| dc.date.Accepted.fl_str_mv | 2016-01-01T00:00:00Z |
| dc.date.available.fl_str_mv | 2017-10-13T08:02:31Z |
| dc.date.embargoed.fl_str_mv | 2017-10-13T08:02:31Z |
| dc.format.none.fl_str_mv | application/pdf |
| dc.identifier.none.fl_str_mv | http://hdl.handle.net/10400.14/23067 |
| dc.language.none.fl_str_mv | eng |
| dc.publisher.none.fl_str_mv | Academic Press |
| dc.rights.none.fl_str_mv | http://purl.org/coar/access_right/c_16ec |
| dc.subject.none.fl_str_mv | Moxifloxacin Fluoroquinolones Biodegradation Acetate Antimicrobial activity |
| dc.title.fl_str_mv | Bacterial degradation of moxifloxacin in the presence of acetate as a bulk substrate |
| dc.type.none.fl_str_mv | http://purl.org/coar/resource_type/c_6501 |
| description | Fluoroquinolones constitute a group of emerging pollutants and their occurrence in different environmental compartments is becoming object of increasing public concern due to their ecotoxicological effects and the potential to develop resistant bacteria. This study aimed to investigate the biodegradation of moxifloxacin (MOX), for which studies in the literature are very scarce. An activated sludge (AS) consortium and three bacterial strains able to degrade fluoroaromatic compounds e strains F11, FP1 and S2 e were tested. Biodegradation studies were conducted using acetate as a bulk carbon source. Strain F11 showed the highest biodegradation capacity, being able to completely consume and dehalogenate 7.5 mM of the target antibiotic when daily co-supplemented with acetate present as a readily degradable organic substrate in wastewaters. MOX could be used by strain F11 as a sole nitrogen source but the presence of an external nitrogen source in the culture medium was essential for complete biodegradation. Strain F11 was capable of completely consuming MOX in a range between 2 and 11 mM, although stoichiometric fluoride release was not obtained for the highest tested concentration. The antibacterial activity of residual MOX and of the metabolic products potentially resultant from the biodegradation process was investigated by agar diffusion tests, demonstrating that MOX biodegradation is associated with the elimination of the antibacterial properties of the target antibiotic and of the produced metabolites, which is an important result, as the activity of antibiotics and/or their metabolites in the environment, even at low levels, may lead to the development of resistant bacterial strains. Overall, the results obtained in this study suggest that strain F11 is a promising microorganism for the treatment of waters contaminated with MOX, where it could be used for bioaugmentation/bioremediation purposes. To the best of our knowledge, this is the first study reporting complete removal and dehalogenation of MOX by a single microorganism. |
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| funding.funder.identifier_str_mv | http://doi.org/10.13039/501100001871 http://doi.org/10.13039/501100001871 |
| funding.funder.name_str_mv | Fundação para a Ciência e a Tecnologia Fundação para a Ciência e a Tecnologia |
| funding.identifier_str_mv | SFRH/BPD/44670/2008 PTDC/EBB-EBI/111699/2009 |
| funding.name_str_mv | 3599-PPCDT |
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| person_str_mv | Carvalho, M. F. Carvalho, M. F. https://www.ciencia-id.pt/6913-1464-155B 6913-1464-155B http://orcid.org/0000-0002-7181-0540 0000-0002-7181-0540 Maia, A. S. Maia, A. S. https://www.ciencia-id.pt/6A15-F0A3-AEF2 6A15-F0A3-AEF2 http://orcid.org/0000-0002-4167-383X 0000-0002-4167-383X Tiritan, M. E. Tiritan, M. E. https://www.ciencia-id.pt/D915-BFC0-FB3A D915-BFC0-FB3A http://orcid.org/0000-0003-3320-730X 0000-0003-3320-730X Castro, P. M. L. Castro, P. M. L. https://www.ciencia-id.pt/7C1F-6C72-354A 7C1F-6C72-354A http://orcid.org/0000-0001-8841-6606 0000-0001-8841-6606 |
| publishDate | 2016 |
| publisher.none.fl_str_mv | Academic Press |
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| spelling | engAcademic Presspt_PTFluoroquinolones constitute a group of emerging pollutants and their occurrence in different environmental compartments is becoming object of increasing public concern due to their ecotoxicological effects and the potential to develop resistant bacteria. This study aimed to investigate the biodegradation of moxifloxacin (MOX), for which studies in the literature are very scarce. An activated sludge (AS) consortium and three bacterial strains able to degrade fluoroaromatic compounds e strains F11, FP1 and S2 e were tested. Biodegradation studies were conducted using acetate as a bulk carbon source. Strain F11 showed the highest biodegradation capacity, being able to completely consume and dehalogenate 7.5 mM of the target antibiotic when daily co-supplemented with acetate present as a readily degradable organic substrate in wastewaters. MOX could be used by strain F11 as a sole nitrogen source but the presence of an external nitrogen source in the culture medium was essential for complete biodegradation. Strain F11 was capable of completely consuming MOX in a range between 2 and 11 mM, although stoichiometric fluoride release was not obtained for the highest tested concentration. The antibacterial activity of residual MOX and of the metabolic products potentially resultant from the biodegradation process was investigated by agar diffusion tests, demonstrating that MOX biodegradation is associated with the elimination of the antibacterial properties of the target antibiotic and of the produced metabolites, which is an important result, as the activity of antibiotics and/or their metabolites in the environment, even at low levels, may lead to the development of resistant bacterial strains. Overall, the results obtained in this study suggest that strain F11 is a promising microorganism for the treatment of waters contaminated with MOX, where it could be used for bioaugmentation/bioremediation purposes. To the best of our knowledge, this is the first study reporting complete removal and dehalogenation of MOX by a single microorganism.application/pdfpt_PTBacterial degradation of moxifloxacin in the presence of acetate as a bulk substratePersonalCarvalho, M. F.DSpacehttp://dspace.org/items/964897d5-02c3-4efe-a8c3-44ea68cf4974DSpacehttp://dspace.org/items/964897d5-02c3-4efe-a8c3-44ea68cf4974CarvalhoM. FátimaCiência IDhttps://www.ciencia-id.pt6913-1464-155BORCIDhttp://orcid.org0000-0002-7181-0540Scopus Author IDhttps://www.scopus.com14038677200PersonalMaia, A. S.DSpacehttp://dspace.org/items/56eedbbf-8105-4d55-bf1f-e38c62ed4da5DSpacehttp://dspace.org/items/56eedbbf-8105-4d55-bf1f-e38c62ed4da5MaiaAlexandra S.Ciência IDhttps://www.ciencia-id.pt6A15-F0A3-AEF2ORCIDhttp://orcid.org0000-0002-4167-383XScopus Author IDhttps://www.scopus.com55332349300PersonalTiritan, M. E.DSpacehttp://dspace.org/items/1ab8e8f5-cf82-4edd-89d8-46e5cca13e19DSpacehttp://dspace.org/items/1ab8e8f5-cf82-4edd-89d8-46e5cca13e19TiritanMaria ElizabethCiência IDhttps://www.ciencia-id.ptD915-BFC0-FB3AORCIDhttp://orcid.org0000-0003-3320-730XResearcher IDhttps://www.researcherid.comK-9492-2014Scopus Author IDhttps://www.scopus.com6602890981PersonalCastro, P. M. L.DSpacehttp://dspace.org/items/f2253992-dc8d-4042-9a0f-597ebcf0a1d6DSpacehttp://dspace.org/items/f2253992-dc8d-4042-9a0f-597ebcf0a1d6CastroPaulaCiência IDhttps://www.ciencia-id.pt7C1F-6C72-354AORCIDhttp://orcid.org0000-0001-8841-6606Researcher IDhttps://www.researcherid.comM-8241-2013Scopus Author IDhttps://www.scopus.com7102781782HostingInstitutionOrganizationalVeritatie-mailmailto:repositorio@ucp.ptrepositorio@ucp.ptDOIIsPartOf10.1016/j.jenvman.2015.12.0102017-10-13T08:02:31Z20162016-01-01T00:00:00ZHandlehttp://hdl.handle.net/10400.14/23067http://purl.org/coar/access_right/c_16ecrestricted accessMoxifloxacinFluoroquinolonesBiodegradationAcetateAntimicrobial activity839604 bytesFundação para a Ciência e a TecnologiaBIOLOGICAL PRODUCTION OF INDUSTRIALLY RELEVANT FLUOROORGANIC COMPOUNDSinfo:eu-repo/grantAgreement/FCT//SFRH%2FBPD%2F44670%2F2008/PTSFRH/BPD/44670/2008Crossref Funder IDhttp://doi.org/10.13039/501100001871Fundação para a Ciência e a TecnologiaFLUOROPHARMA - Biodegradation and removal of chiral and achiral fluorinated pharmaceuticals from wastewatersinfo:eu-repo/grantAgreement/FCT/3599-PPCDT/PTDC%2FEBB-EBI%2F111699%2F2009/PTPTDC/EBB-EBI/111699/20093599-PPCDTCrossref Funder IDhttp://doi.org/10.13039/501100001871literaturehttp://purl.org/coar/resource_type/c_6501journal articlehttp://purl.org/coar/access_right/c_16ecapplication/pdffulltexthttps://repositorio.ucp.pt/bitstreams/0ff1d460-8838-4d7c-9f40-ff1120eb54a2/downloadJournal of Environmental Management219228 |
| spellingShingle | Bacterial degradation of moxifloxacin in the presence of acetate as a bulk substrate Carvalho, M. F. Moxifloxacin Fluoroquinolones Biodegradation Acetate Antimicrobial activity |
| status | SINGLETON |
| subject.fl_str_mv | Moxifloxacin Fluoroquinolones Biodegradation Acetate Antimicrobial activity |
| title | Bacterial degradation of moxifloxacin in the presence of acetate as a bulk substrate |
| title_full | Bacterial degradation of moxifloxacin in the presence of acetate as a bulk substrate |
| title_fullStr | Bacterial degradation of moxifloxacin in the presence of acetate as a bulk substrate |
| title_full_unstemmed | Bacterial degradation of moxifloxacin in the presence of acetate as a bulk substrate |
| title_short | Bacterial degradation of moxifloxacin in the presence of acetate as a bulk substrate |
| title_sort | Bacterial degradation of moxifloxacin in the presence of acetate as a bulk substrate |
| topic | Moxifloxacin Fluoroquinolones Biodegradation Acetate Antimicrobial activity |
| topic_facet | Moxifloxacin Fluoroquinolones Biodegradation Acetate Antimicrobial activity |
| url | http://hdl.handle.net/10400.14/23067 |
| visible | 1 |