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Degradation kinetics of peroxidase enzyme, phenolic content, and physical and sensorial characteristics in broccoli (brassica oleracea L. ssp. Italica) during blanching

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Summary:The effects of water blanching treatment on peroxidase inactivation, total phenolic content, color parameters [-a*/b* and hue (h *)], texture (maximum shear force), and sensory attributes (color and texture, evaluated by a trained panel) of broccoli (Brassica oleracea L. ssp. Italica) were studied at five temperatures (70, 75, 80, 85, and 90 C). Experimental results showed that all studied broccoli quality parameters suffered significative changes due to blanching treatments. The vegetal total phenolic content showed a marked decline. Degradation on objective color and texture measurements and alterations in sensorial attributes were detected. Correlations between sensory and instrumental measurements have been found. Under the conditions 70 C and 6.5 min or 90 C and 0.4 min, 90% of the initial peroxidase activity was reduced. At these conditions, no significant alterations were detected by panelists, and a small amount of phenolic content was lost (ca. 16 and 10%, respectively). The peroxidase inactivation and phenolic content degradation were found to follow first-order reaction models. The zero-order reaction model showed a good fit to the broccoli color (-a*/b* and h *), texture, and sensory parameters changes. The temperature effect was welldescribed by the Arrhenius law.
Main Authors:Gonçalves, Elsa M.
Other Authors:Pinheiro, Joaquina; Alegria, C.; Abreu, Marta; Brandão, Teresa R. S.; Silva, Cristina L.M.
Subject:Broccoli Blanching Quality parameters Kinetics modeling
Year:2009
Country:Portugal
Document type:article
Access type:restricted access
Associated institution:Universidade Católica Portuguesa
Language:English
Origin:Veritati - Repositório Institucional da Universidade Católica Portuguesa
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author Gonçalves, Elsa M.
author2 Pinheiro, Joaquina
Alegria, C.
Abreu, Marta
Brandão, Teresa R. S.
Silva, Cristina L.M.
author2_role author
author
author
author
author
author_facet Gonçalves, Elsa M.
Pinheiro, Joaquina
Alegria, C.
Abreu, Marta
Brandão, Teresa R. S.
Silva, Cristina L.M.
author_role author
contributor_name_str_mv Veritati
country_str PT
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datacite.contributors.contributor.contributorName.fl_str_mv Veritati
datacite.creators.creator.creatorName.fl_str_mv Gonçalves, Elsa M.
Pinheiro, Joaquina
Alegria, C.
Abreu, Marta
Brandão, Teresa R. S.
Silva, Cristina L.M.
datacite.date.Accepted.fl_str_mv 2009-01-01T00:00:00Z
datacite.date.available.fl_str_mv 2010-10-07T16:02:10Z
datacite.date.embargoed.fl_str_mv 2010-10-07T16:02:10Z
datacite.rights.fl_str_mv http://purl.org/coar/access_right/c_16ec
datacite.subjects.subject.fl_str_mv Broccoli
Blanching
Quality parameters
Kinetics modeling
datacite.titles.title.fl_str_mv Degradation kinetics of peroxidase enzyme, phenolic content, and physical and sensorial characteristics in broccoli (brassica oleracea L. ssp. Italica) during blanching
dc.contributor.none.fl_str_mv Veritati
dc.creator.none.fl_str_mv Gonçalves, Elsa M.
Pinheiro, Joaquina
Alegria, C.
Abreu, Marta
Brandão, Teresa R. S.
Silva, Cristina L.M.
dc.date.Accepted.fl_str_mv 2009-01-01T00:00:00Z
dc.date.available.fl_str_mv 2010-10-07T16:02:10Z
dc.date.embargoed.fl_str_mv 2010-10-07T16:02:10Z
dc.format.none.fl_str_mv application/pdf
dc.identifier.none.fl_str_mv http://hdl.handle.net/10400.14/2701
dc.language.none.fl_str_mv eng
dc.publisher.none.fl_str_mv American Chemical Society
dc.rights.none.fl_str_mv http://purl.org/coar/access_right/c_16ec
dc.subject.none.fl_str_mv Broccoli
Blanching
Quality parameters
Kinetics modeling
dc.title.fl_str_mv Degradation kinetics of peroxidase enzyme, phenolic content, and physical and sensorial characteristics in broccoli (brassica oleracea L. ssp. Italica) during blanching
dc.type.none.fl_str_mv http://purl.org/coar/resource_type/c_6501
description The effects of water blanching treatment on peroxidase inactivation, total phenolic content, color parameters [-a*/b* and hue (h *)], texture (maximum shear force), and sensory attributes (color and texture, evaluated by a trained panel) of broccoli (Brassica oleracea L. ssp. Italica) were studied at five temperatures (70, 75, 80, 85, and 90 C). Experimental results showed that all studied broccoli quality parameters suffered significative changes due to blanching treatments. The vegetal total phenolic content showed a marked decline. Degradation on objective color and texture measurements and alterations in sensorial attributes were detected. Correlations between sensory and instrumental measurements have been found. Under the conditions 70 C and 6.5 min or 90 C and 0.4 min, 90% of the initial peroxidase activity was reduced. At these conditions, no significant alterations were detected by panelists, and a small amount of phenolic content was lost (ca. 16 and 10%, respectively). The peroxidase inactivation and phenolic content degradation were found to follow first-order reaction models. The zero-order reaction model showed a good fit to the broccoli color (-a*/b* and h *), texture, and sensory parameters changes. The temperature effect was welldescribed by the Arrhenius law.
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organization_str_mv urn:organizationAcronym:ucp
person_str_mv Gonçalves, Elsa M.
Gonçalves, Elsa M.
https://www.ciencia-id.pt/AA12-42D6-6D59
AA12-42D6-6D59
http://orcid.org/0000-0002-5814-0490
0000-0002-5814-0490
Pinheiro, Joaquina
Pinheiro, Joaquina
https://www.ciencia-id.pt/B113-2D80-406B
B113-2D80-406B
http://orcid.org/0000-0001-9319-2651
0000-0001-9319-2651
Alegria, C.
Alegria, C.
https://www.ciencia-id.pt/5115-6CF2-C01A
5115-6CF2-C01A
http://orcid.org/0000-0002-9461-4569
0000-0002-9461-4569
Abreu, Marta
Abreu, Marta
https://www.ciencia-id.pt/A31F-D2A3-36A8
A31F-D2A3-36A8
http://orcid.org/0000-0002-0245-7122
0000-0002-0245-7122
Brandão, Teresa R. S.
Brandão, Teresa R. S.
https://www.ciencia-id.pt/7B19-E23B-BB31
7B19-E23B-BB31
http://orcid.org/0000-0002-8857-6471
0000-0002-8857-6471
Silva, Cristina L.M.
Silva, Cristina L.M.
https://www.ciencia-id.pt/6611-634A-132C
6611-634A-132C
http://orcid.org/0000-0002-0495-3955
0000-0002-0495-3955
publishDate 2009
publisher.none.fl_str_mv American Chemical Society
repo_facet_str urn:repositoryAcronym:veritati{{{_:::_}}}Veritati - Repositório Institucional da Universidade Católica Portuguesa
reponame_str Veritati - Repositório Institucional da Universidade Católica Portuguesa
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spelling engAmerican Chemical SocietyporThe effects of water blanching treatment on peroxidase inactivation, total phenolic content, color parameters [-a*/b* and hue (h *)], texture (maximum shear force), and sensory attributes (color and texture, evaluated by a trained panel) of broccoli (Brassica oleracea L. ssp. Italica) were studied at five temperatures (70, 75, 80, 85, and 90 C). Experimental results showed that all studied broccoli quality parameters suffered significative changes due to blanching treatments. The vegetal total phenolic content showed a marked decline. Degradation on objective color and texture measurements and alterations in sensorial attributes were detected. Correlations between sensory and instrumental measurements have been found. Under the conditions 70 C and 6.5 min or 90 C and 0.4 min, 90% of the initial peroxidase activity was reduced. At these conditions, no significant alterations were detected by panelists, and a small amount of phenolic content was lost (ca. 16 and 10%, respectively). The peroxidase inactivation and phenolic content degradation were found to follow first-order reaction models. The zero-order reaction model showed a good fit to the broccoli color (-a*/b* and h *), texture, and sensory parameters changes. The temperature effect was welldescribed by the Arrhenius law.application/pdfporDegradation kinetics of peroxidase enzyme, phenolic content, and physical and sensorial characteristics in broccoli (brassica oleracea L. ssp. Italica) during blanchingPersonalGonçalves, Elsa M.DSpacehttp://dspace.org/items/cb65b8c9-419a-4bbb-ab94-4b9197ff9585DSpacehttp://dspace.org/items/cb65b8c9-419a-4bbb-ab94-4b9197ff9585GonçalvesElsa MargaridaCiência IDhttps://www.ciencia-id.ptAA12-42D6-6D59ORCIDhttp://orcid.org0000-0002-5814-0490Researcher IDhttps://www.researcherid.comN-4198-2013Scopus Author IDhttps://www.scopus.com7004990784PersonalPinheiro, JoaquinaDSpacehttp://dspace.org/items/2e82d021-812c-4df6-839c-cc62fe1f1861DSpacehttp://dspace.org/items/2e82d021-812c-4df6-839c-cc62fe1f1861PinheiroJoaquinaCiência IDhttps://www.ciencia-id.ptB113-2D80-406BORCIDhttp://orcid.org0000-0001-9319-2651Scopus Author IDhttps://www.scopus.com25228814000PersonalAlegria, C.DSpacehttp://dspace.org/items/f0d4301e-0bdc-41e0-80c6-e096c1e32a33DSpacehttp://dspace.org/items/f0d4301e-0bdc-41e0-80c6-e096c1e32a33AlegriaCarlaCiência IDhttps://www.ciencia-id.pt5115-6CF2-C01AORCIDhttp://orcid.org0000-0002-9461-4569Scopus Author IDhttps://www.scopus.com25228203500PersonalAbreu, MartaDSpacehttp://dspace.org/items/de86ca0a-74b6-490e-9466-5b5d31ce948fDSpacehttp://dspace.org/items/de86ca0a-74b6-490e-9466-5b5d31ce948fAbreuMartaCiência IDhttps://www.ciencia-id.ptA31F-D2A3-36A8ORCIDhttp://orcid.org0000-0002-0245-7122Scopus Author IDhttps://www.scopus.com7102768632PersonalBrandão, Teresa R. S.DSpacehttp://dspace.org/items/ebf7ead0-3772-4fd6-988c-f6b8722de84eDSpacehttp://dspace.org/items/ebf7ead0-3772-4fd6-988c-f6b8722de84eBrandãoTeresaCiência IDhttps://www.ciencia-id.pt7B19-E23B-BB31ORCIDhttp://orcid.org0000-0002-8857-6471Researcher IDhttps://www.researcherid.comJ-9418-2012Scopus Author IDhttps://www.scopus.com6602174128Scopus Author IDhttps://www.scopus.com6602174128PersonalSilva, Cristina L.M.DSpacehttp://dspace.org/items/4e2903de-0679-4cb1-981e-10de14c0c995DSpacehttp://dspace.org/items/4e2903de-0679-4cb1-981e-10de14c0c995SilvaCristinaCiência IDhttps://www.ciencia-id.pt6611-634A-132CORCIDhttp://orcid.org0000-0002-0495-3955Researcher IDhttps://www.researcherid.comA-3568-2013Scopus Author IDhttps://www.scopus.com7201387397Scopus Author IDhttps://www.scopus.com7201387397HostingInstitutionOrganizationalVeritatie-mailmailto:repositorio@ucp.ptrepositorio@ucp.ptDOIIsPartOf10.1021/jf900314x2010-10-07T16:02:10Z20092009-01-01T00:00:00ZHandlehttp://hdl.handle.net/10400.14/2701http://purl.org/coar/access_right/c_16ecrestricted accessBroccoliBlanchingQuality parametersKinetics modeling885001 bytesliteraturehttp://purl.org/coar/resource_type/c_6501journal articlehttp://purl.org/coar/access_right/c_16ecapplication/pdffulltexthttps://repositorio.ucp.pt/bitstreams/5a66d33c-3fd5-4b07-9345-ba13999f2871/download
spellingShingle Degradation kinetics of peroxidase enzyme, phenolic content, and physical and sensorial characteristics in broccoli (brassica oleracea L. ssp. Italica) during blanching
Gonçalves, Elsa M.
Broccoli
Blanching
Quality parameters
Kinetics modeling
status SINGLETON
subject.fl_str_mv Broccoli
Blanching
Quality parameters
Kinetics modeling
title Degradation kinetics of peroxidase enzyme, phenolic content, and physical and sensorial characteristics in broccoli (brassica oleracea L. ssp. Italica) during blanching
title_full Degradation kinetics of peroxidase enzyme, phenolic content, and physical and sensorial characteristics in broccoli (brassica oleracea L. ssp. Italica) during blanching
title_fullStr Degradation kinetics of peroxidase enzyme, phenolic content, and physical and sensorial characteristics in broccoli (brassica oleracea L. ssp. Italica) during blanching
title_full_unstemmed Degradation kinetics of peroxidase enzyme, phenolic content, and physical and sensorial characteristics in broccoli (brassica oleracea L. ssp. Italica) during blanching
title_short Degradation kinetics of peroxidase enzyme, phenolic content, and physical and sensorial characteristics in broccoli (brassica oleracea L. ssp. Italica) during blanching
title_sort Degradation kinetics of peroxidase enzyme, phenolic content, and physical and sensorial characteristics in broccoli (brassica oleracea L. ssp. Italica) during blanching
topic Broccoli
Blanching
Quality parameters
Kinetics modeling
topic_facet Broccoli
Blanching
Quality parameters
Kinetics modeling
url http://hdl.handle.net/10400.14/2701
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