Publicação
Athletes have more susceptibility to oxidative stress : truth or myth? A study in swimmers
| Resumo: | Aims. — Exercise is associated with accelerated reactive oxygen species (ROS) generation and stimulation of the antioxidant defences (AD). In high intensity exercise ROS production may overwhelm the AD and induce adverse effects on health and performance. We aim to investigate the redox balance of adult-competitive swimmers against recreational exercise practitioners. Methods. — Fifteen high-competition male swimmers (S) and 16 active men (AM), all aged between 18—25 years, participated in the study. Venous blood was collected at rest in the morning to assess oxidative and muscle damage markers, antioxidant enzymatic activities in erythrocytes, and non-enzymatic antioxidants in plasma. All parameters were assessed by photometry with the exception of thiobarbituric acid reactive substances (TBARS), assessed by fluorimetry. Bivariate correlations were performed to investigate the association between all redox balance markers. Partial correlation analysis was conducted to control for the exercise level. Results. — Differences between groups were observed in red blood cells peroxidation (RBCPx—S: 39.2 ± 4.8; AM: 46.3 ± 9.5%) and in meta-hemoglobin reductase (RmHb — S: 7.5 ± 2.1; AM: 9.1 ± 2.3mol/min.gHb), with S presenting lower mean values (P < 0.05). Controlling for the exercise level, adrenaline oxidase (AdOx) was inversely correlated with carotenoids (R = −0.596; P = 0.001) and directly with creatine kinase (CK) (R = 0.413; P = 0.045) and with TBARS (R = 0.532; P = 0.005); RBCPx was inversely correlated with acid phosphatase (ACP1) (R = −0.392; P = 0.048) and directly with glutathione reductase (GR) (R = 0.524; P = 0.006); GR was inversely correlated with TBARS (R = −0.404; P = 0.041); VitE was directly correlated with Carotenoids (R = 0.723; P = 0.001) and inversely with TBARS (R = 0.543; P = 0.004). Therefore: AD are effective at controlling ROS; VitE and carotenoids are important protective lipid antioxidants; higher TBARS may be associated to the stimulation of GR activity; muscle leakages associated to the AdOx activity which may result from ion leakage, intravascular hemolysis or inflammation. Conclusion. — Our results suggest that competitive swimmers are not at higher risk of oxidative damage than recreational exercise practitioners. |
|---|---|
| Autores principais: | Matias, Catarina Nunes |
| Outros Autores: | Bicho, Manuel; Laires, Maria José; Monteiro, Cristina P. |
| Assunto: | Antioxidants Reactive oxygen species Athletes Cardiorespiratory fitness |
| Ano: | 2020 |
| País: | Portugal |
| Tipo de documento: | artigo |
| Tipo de acesso: | acesso restrito |
| Instituição associada: | Universidade de Lisboa |
| Idioma: | inglês |
| Origem: | Repositório da Universidade de Lisboa |
| _version_ | 1877771029406810112 |
|---|---|
| author | Matias, Catarina Nunes |
| author2 | Bicho, Manuel Laires, Maria José Monteiro, Cristina P. |
| author2_role | author author author |
| author_facet | Matias, Catarina Nunes Bicho, Manuel Laires, Maria José Monteiro, Cristina P. |
| author_role | author |
| contributor_name_str_mv | Repositório Científico de Acesso Aberto da ULisboa |
| country_str | PT |
| creators_json_txt | [{\"Person.name\":\"Matias, Catarina Nunes\"},{\"Person.name\":\"Bicho, Manuel\",\"Person.identifier.cienciaID\":\"9512-8D22-1B28\",\"Person.identifier.orcid\":\"0000-0002-5773-5687\",\"Person.identifier.scopusAuthorID\":\"7004153755\"},{\"Person.name\":\"Laires, Maria José\",\"Person.identifier.orcid\":\"0000-0001-8453-9123\",\"Person.identifier.scopusAuthorID\":\"6602311659\"},{\"Person.name\":\"Monteiro, Cristina P.\",\"Person.identifier.cienciaID\":\"761C-4539-6E9E\",\"Person.identifier.orcid\":\"0000-0003-2036-7988\",\"Person.identifier.scopusAuthorID\":\"7103263077\"}] |
| datacite.contributors.contributor.contributorName.fl_str_mv | Repositório Científico de Acesso Aberto da ULisboa |
| datacite.creators.creator.creatorName.fl_str_mv | Matias, Catarina Nunes Bicho, Manuel Laires, Maria José Monteiro, Cristina P. |
| datacite.date.Accepted.fl_str_mv | 2020-01-01T00:00:00Z |
| datacite.date.available.fl_str_mv | 2020-02-26T15:19:19Z |
| datacite.date.embargoed.fl_str_mv | 2020-02-26T15:19:19Z |
| datacite.rights.fl_str_mv | http://purl.org/coar/access_right/c_16ec |
| datacite.subjects.subject.fl_str_mv | Antioxidants Reactive oxygen species Athletes Cardiorespiratory fitness |
| datacite.titles.title.fl_str_mv | Athletes have more susceptibility to oxidative stress : truth or myth? A study in swimmers Susceptibilité accrue des athlètes au stress oxydant : réalité ou mythe? Étude chez des nageurs |
| dc.contributor.none.fl_str_mv | Repositório Científico de Acesso Aberto da ULisboa |
| dc.creator.none.fl_str_mv | Matias, Catarina Nunes Bicho, Manuel Laires, Maria José Monteiro, Cristina P. |
| dc.date.Accepted.fl_str_mv | 2020-01-01T00:00:00Z |
| dc.date.available.fl_str_mv | 2020-02-26T15:19:19Z |
| dc.date.embargoed.fl_str_mv | 2020-02-26T15:19:19Z |
| dc.format.none.fl_str_mv | application/pdf |
| dc.identifier.none.fl_str_mv | http://hdl.handle.net/10451/42026 |
| dc.language.none.fl_str_mv | eng |
| dc.publisher.none.fl_str_mv | Elsevier Masson |
| dc.rights.none.fl_str_mv | http://purl.org/coar/access_right/c_16ec |
| dc.subject.none.fl_str_mv | Antioxidants Reactive oxygen species Athletes Cardiorespiratory fitness |
| dc.title.fl_str_mv | Athletes have more susceptibility to oxidative stress : truth or myth? A study in swimmers Susceptibilité accrue des athlètes au stress oxydant : réalité ou mythe? Étude chez des nageurs |
| dc.type.none.fl_str_mv | http://purl.org/coar/resource_type/c_6501 |
| description | Aims. — Exercise is associated with accelerated reactive oxygen species (ROS) generation and stimulation of the antioxidant defences (AD). In high intensity exercise ROS production may overwhelm the AD and induce adverse effects on health and performance. We aim to investigate the redox balance of adult-competitive swimmers against recreational exercise practitioners. Methods. — Fifteen high-competition male swimmers (S) and 16 active men (AM), all aged between 18—25 years, participated in the study. Venous blood was collected at rest in the morning to assess oxidative and muscle damage markers, antioxidant enzymatic activities in erythrocytes, and non-enzymatic antioxidants in plasma. All parameters were assessed by photometry with the exception of thiobarbituric acid reactive substances (TBARS), assessed by fluorimetry. Bivariate correlations were performed to investigate the association between all redox balance markers. Partial correlation analysis was conducted to control for the exercise level. Results. — Differences between groups were observed in red blood cells peroxidation (RBCPx—S: 39.2 ± 4.8; AM: 46.3 ± 9.5%) and in meta-hemoglobin reductase (RmHb — S: 7.5 ± 2.1; AM: 9.1 ± 2.3mol/min.gHb), with S presenting lower mean values (P < 0.05). Controlling for the exercise level, adrenaline oxidase (AdOx) was inversely correlated with carotenoids (R = −0.596; P = 0.001) and directly with creatine kinase (CK) (R = 0.413; P = 0.045) and with TBARS (R = 0.532; P = 0.005); RBCPx was inversely correlated with acid phosphatase (ACP1) (R = −0.392; P = 0.048) and directly with glutathione reductase (GR) (R = 0.524; P = 0.006); GR was inversely correlated with TBARS (R = −0.404; P = 0.041); VitE was directly correlated with Carotenoids (R = 0.723; P = 0.001) and inversely with TBARS (R = 0.543; P = 0.004). Therefore: AD are effective at controlling ROS; VitE and carotenoids are important protective lipid antioxidants; higher TBARS may be associated to the stimulation of GR activity; muscle leakages associated to the AdOx activity which may result from ion leakage, intravascular hemolysis or inflammation. Conclusion. — Our results suggest that competitive swimmers are not at higher risk of oxidative damage than recreational exercise practitioners. |
| dirty | 0 |
| eu_rights_str_mv | restrictedAccess |
| format | article |
| fulltext.url.fl_str_mv | https://repositorio.ulisboa.pt/bitstreams/a105bee5-5869-4fd0-86a1-b97748a170d3/download |
| id | ul_d7c5b03c124f404e9bcae6c0dc8e46dd |
| identifier.url.fl_str_mv | http://hdl.handle.net/10451/42026 |
| inst_facet_str | urn:organizationAcronym:ul{{{_:::_}}}Universidade de Lisboa |
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| institution | Universidade de Lisboa |
| instname_str | Universidade de Lisboa |
| language | eng |
| network_acronym_str | ul |
| network_name_str | Repositório da Universidade de Lisboa |
| oai_identifier_str | oai:repositorio.ulisboa.pt:10451/42026 |
| organization_str_mv | urn:organizationAcronym:ul |
| person_str_mv | Matias, Catarina Nunes Bicho, Manuel Bicho, Manuel https://www.ciencia-id.pt/9512-8D22-1B28 9512-8D22-1B28 http://orcid.org/0000-0002-5773-5687 0000-0002-5773-5687 Laires, Maria José Laires, Maria José http://orcid.org/0000-0001-8453-9123 0000-0001-8453-9123 Monteiro, Cristina P. Monteiro, Cristina P. https://www.ciencia-id.pt/761C-4539-6E9E 761C-4539-6E9E http://orcid.org/0000-0003-2036-7988 0000-0003-2036-7988 |
| publishDate | 2020 |
| publisher.none.fl_str_mv | Elsevier Masson |
| repo_facet_str | urn:repositoryAcronym:ul{{{_:::_}}}Repositório da Universidade de Lisboa |
| reponame_str | Repositório da Universidade de Lisboa |
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| spelling | engElsevier Massonpt_PTAims. — Exercise is associated with accelerated reactive oxygen species (ROS) generation and stimulation of the antioxidant defences (AD). In high intensity exercise ROS production may overwhelm the AD and induce adverse effects on health and performance. We aim to investigate the redox balance of adult-competitive swimmers against recreational exercise practitioners. Methods. — Fifteen high-competition male swimmers (S) and 16 active men (AM), all aged between 18—25 years, participated in the study. Venous blood was collected at rest in the morning to assess oxidative and muscle damage markers, antioxidant enzymatic activities in erythrocytes, and non-enzymatic antioxidants in plasma. All parameters were assessed by photometry with the exception of thiobarbituric acid reactive substances (TBARS), assessed by fluorimetry. Bivariate correlations were performed to investigate the association between all redox balance markers. Partial correlation analysis was conducted to control for the exercise level. Results. — Differences between groups were observed in red blood cells peroxidation (RBCPx—S: 39.2 ± 4.8; AM: 46.3 ± 9.5%) and in meta-hemoglobin reductase (RmHb — S: 7.5 ± 2.1; AM: 9.1 ± 2.3mol/min.gHb), with S presenting lower mean values (P < 0.05). Controlling for the exercise level, adrenaline oxidase (AdOx) was inversely correlated with carotenoids (R = −0.596; P = 0.001) and directly with creatine kinase (CK) (R = 0.413; P = 0.045) and with TBARS (R = 0.532; P = 0.005); RBCPx was inversely correlated with acid phosphatase (ACP1) (R = −0.392; P = 0.048) and directly with glutathione reductase (GR) (R = 0.524; P = 0.006); GR was inversely correlated with TBARS (R = −0.404; P = 0.041); VitE was directly correlated with Carotenoids (R = 0.723; P = 0.001) and inversely with TBARS (R = 0.543; P = 0.004). Therefore: AD are effective at controlling ROS; VitE and carotenoids are important protective lipid antioxidants; higher TBARS may be associated to the stimulation of GR activity; muscle leakages associated to the AdOx activity which may result from ion leakage, intravascular hemolysis or inflammation. Conclusion. — Our results suggest that competitive swimmers are not at higher risk of oxidative damage than recreational exercise practitioners.application/pdfpt_PTAthletes have more susceptibility to oxidative stress : truth or myth? A study in swimmersAlternativeTitlept_PTSusceptibilité accrue des athlètes au stress oxydant : réalité ou mythe? Étude chez des nageursMatias, Catarina NunesPersonalBicho, ManuelDSpacehttp://dspace.org/items/c61e4f7e-28b3-47b4-bc5d-677b73fc3aecDSpacehttp://dspace.org/items/c61e4f7e-28b3-47b4-bc5d-677b73fc3aecBichoManuelCiência IDhttps://www.ciencia-id.pt9512-8D22-1B28ORCIDhttp://orcid.org0000-0002-5773-5687Scopus Author IDhttps://www.scopus.com7004153755PersonalLaires, Maria JoséDSpacehttp://dspace.org/items/916c78bd-6fd1-4c99-9fe9-6bf68eb29e3dDSpacehttp://dspace.org/items/916c78bd-6fd1-4c99-9fe9-6bf68eb29e3dLairesMaria JoséORCIDhttp://orcid.org0000-0001-8453-9123Scopus Author IDhttps://www.scopus.com6602311659PersonalMonteiro, Cristina P.DSpacehttp://dspace.org/items/79d2a5c5-5f80-434c-b1f5-8010fe1046f2DSpacehttp://dspace.org/items/79d2a5c5-5f80-434c-b1f5-8010fe1046f2Ciência IDhttps://www.ciencia-id.pt761C-4539-6E9EORCIDhttp://orcid.org0000-0003-2036-7988Scopus Author IDhttps://www.scopus.com7103263077HostingInstitutionOrganizationalRepositório Científico de Acesso Aberto da ULisboae-mailmailto:repositorio@ulisboa.ptrepositorio@ulisboa.ptISSNIsPartOf0765-1597DOIIsPartOf10.1016/j.scispo.2018.11.0082020-02-26T15:19:19Z20202020-01-01T00:00:00ZHandlehttp://hdl.handle.net/10451/42026http://purl.org/coar/access_right/c_16ecrestricted accessAntioxidantsReactive oxygen speciesAthletesCardiorespiratory fitness449117 bytesliteraturehttp://purl.org/coar/resource_type/c_6501journal articlehttp://purl.org/coar/access_right/c_16ecapplication/pdffulltexthttps://repositorio.ulisboa.pt/bitstreams/a105bee5-5869-4fd0-86a1-b97748a170d3/downloadScience & Sports3512028 |
| spellingShingle | Athletes have more susceptibility to oxidative stress : truth or myth? A study in swimmers Matias, Catarina Nunes Antioxidants Reactive oxygen species Athletes Cardiorespiratory fitness |
| status | SINGLETON |
| subject.fl_str_mv | Antioxidants Reactive oxygen species Athletes Cardiorespiratory fitness |
| title | Athletes have more susceptibility to oxidative stress : truth or myth? A study in swimmers |
| title_full | Athletes have more susceptibility to oxidative stress : truth or myth? A study in swimmers |
| title_fullStr | Athletes have more susceptibility to oxidative stress : truth or myth? A study in swimmers |
| title_full_unstemmed | Athletes have more susceptibility to oxidative stress : truth or myth? A study in swimmers |
| title_short | Athletes have more susceptibility to oxidative stress : truth or myth? A study in swimmers |
| title_sort | Athletes have more susceptibility to oxidative stress : truth or myth? A study in swimmers |
| topic | Antioxidants Reactive oxygen species Athletes Cardiorespiratory fitness |
| topic_facet | Antioxidants Reactive oxygen species Athletes Cardiorespiratory fitness |
| url | http://hdl.handle.net/10451/42026 |
| visible | 1 |
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