Publicação
Chemical Profile and Biological Activities of Brassica rapa and Brassica napus Ex Situ Collection from Portugal
| Resumo: | This study aimed to analyse the chemical profile and biological activities of 29 accessions of Brassica rapa (turnips) and 9 of Brassica napus (turnips and seeds) collections, maintained ex situ in Por- tugal. HPLC-HRMS allowed the determination of glucosinolates (GLS) and polyphenolic compounds. The antioxidant and antimicrobial activities were determined by using relevant assays. The chemical profiles showed that glucosamine, gluconasturtiin, and neoglucobrassin were the most abundant GLS in the extracts from the turnip accessions. Minor forms of GLS include gluconapoleiferin, glucobrassicanapin, glucoerucin, glucobrassin, and 4-hydroxyglucobrassin. Both species exhibited strong antioxidant activity, attributed to glucosinolates and phenolic compounds. The methanol extracts of Brassica rapa accessions were assessed against a panel of five Gram-negative bacteria (Enterobacter cloacae, Escherichia coli, Pseudomonas aeruginosa, Salmonella enterica subsp. enterica serovar, and Yersinia enterocolitica) and three Gram-positive bacteria (Bacillus cereus, Listeria monocytogenes, and Staphylococcus aureus). The extracts exhibited activity against S. enterica and S. aureus, and two showed inhibitory activity against E. coli and Y. enterocolitica. This study provides valuable insights into the chemical composition and biological properties of Brassica rapa and Brassica napus collections in Portugal. The selected accessions can constitute potential sources of natural antioxidants and bioactive compounds, which can be used in breeding programs and improving human health and to promote healthy food systems. |
|---|---|
| Autores principais: | Serrano, Carmo |
| Outros Autores: | Oliveira, M. Conceição; Lopes, V. R.; Soares, Andreia; Molina, Adriana K.; Paschoalinotto, Beatriz H.; Pires, Tânia C. S. P.; Serra, Octávio; Barata, Ana M. |
| Assunto: | Brassica rapa Brassica napus glucosinolates phenolic compounds antibacterial activity antioxidant activity |
| Ano: | 2024 |
| País: | Portugal |
| Tipo de documento: | artigo |
| Tipo de acesso: | acesso aberto |
| Instituição associada: | Universidade de Lisboa |
| Idioma: | inglês |
| Origem: | Repositório da Universidade de Lisboa |
| _version_ | 1877771463504691200 |
|---|---|
| author | Serrano, Carmo |
| author2 | Oliveira, M. Conceição Lopes, V. R. Soares, Andreia Molina, Adriana K. Paschoalinotto, Beatriz H. Pires, Tânia C. S. P. Serra, Octávio Barata, Ana M. |
| author2_role | author author author author author author author author |
| author_facet | Serrano, Carmo Oliveira, M. Conceição Lopes, V. R. Soares, Andreia Molina, Adriana K. Paschoalinotto, Beatriz H. Pires, Tânia C. S. P. Serra, Octávio Barata, Ana M. |
| author_role | author |
| contributor_name_str_mv | Repositório Científico de Acesso Aberto da ULisboa |
| country_str | PT |
| creators_json_txt | [{\"Person.name\":\"Serrano, Carmo\",\"Person.identifier.cienciaID\":\"5713-9F35-DCDF\",\"Person.identifier.orcid\":\"0000-0002-7632-3485\",\"Person.identifier.scopusAuthorID\":\"41862528200\"},{\"Person.name\":\"Oliveira, M. Conceição\"},{\"Person.name\":\"Lopes, V. R.\"},{\"Person.name\":\"Soares, Andreia\"},{\"Person.name\":\"Molina, Adriana K.\"},{\"Person.name\":\"Paschoalinotto, Beatriz H.\"},{\"Person.name\":\"Pires, Tânia C. S. P.\"},{\"Person.name\":\"Serra, Octávio\"},{\"Person.name\":\"Barata, Ana M.\"}] |
| datacite.contributors.contributor.contributorName.fl_str_mv | Repositório Científico de Acesso Aberto da ULisboa |
| datacite.creators.creator.creatorName.fl_str_mv | Serrano, Carmo Oliveira, M. Conceição Lopes, V. R. Soares, Andreia Molina, Adriana K. Paschoalinotto, Beatriz H. Pires, Tânia C. S. P. Serra, Octávio Barata, Ana M. |
| datacite.date.Accepted.fl_str_mv | 2024-04-01T00:00:00Z |
| datacite.date.available.fl_str_mv | 2025-07-23T15:08:36Z |
| datacite.date.embargoed.fl_str_mv | 2025-07-23T15:08:36Z |
| datacite.rights.fl_str_mv | http://purl.org/coar/access_right/c_abf2 |
| datacite.subjects.subject.fl_str_mv | Brassica rapa Brassica napus glucosinolates phenolic compounds antibacterial activity antioxidant activity |
| datacite.titles.title.fl_str_mv | Chemical Profile and Biological Activities of Brassica rapa and Brassica napus Ex Situ Collection from Portugal |
| dc.contributor.none.fl_str_mv | Repositório Científico de Acesso Aberto da ULisboa |
| dc.creator.none.fl_str_mv | Serrano, Carmo Oliveira, M. Conceição Lopes, V. R. Soares, Andreia Molina, Adriana K. Paschoalinotto, Beatriz H. Pires, Tânia C. S. P. Serra, Octávio Barata, Ana M. |
| dc.date.Accepted.fl_str_mv | 2024-04-01T00:00:00Z |
| dc.date.available.fl_str_mv | 2025-07-23T15:08:36Z |
| dc.date.embargoed.fl_str_mv | 2025-07-23T15:08:36Z |
| dc.format.none.fl_str_mv | application/pdf |
| dc.identifier.none.fl_str_mv | http://hdl.handle.net/10400.5/102386 |
| dc.language.none.fl_str_mv | eng |
| dc.publisher.none.fl_str_mv | MDPI |
| dc.rights.cclincense.fl_str_mv | http://creativecommons.org/licenses/by/4.0/ |
| dc.rights.none.fl_str_mv | http://purl.org/coar/access_right/c_abf2 |
| dc.subject.none.fl_str_mv | Brassica rapa Brassica napus glucosinolates phenolic compounds antibacterial activity antioxidant activity |
| dc.title.fl_str_mv | Chemical Profile and Biological Activities of Brassica rapa and Brassica napus Ex Situ Collection from Portugal |
| dc.type.none.fl_str_mv | http://purl.org/coar/resource_type/c_6501 |
| description | This study aimed to analyse the chemical profile and biological activities of 29 accessions of Brassica rapa (turnips) and 9 of Brassica napus (turnips and seeds) collections, maintained ex situ in Por- tugal. HPLC-HRMS allowed the determination of glucosinolates (GLS) and polyphenolic compounds. The antioxidant and antimicrobial activities were determined by using relevant assays. The chemical profiles showed that glucosamine, gluconasturtiin, and neoglucobrassin were the most abundant GLS in the extracts from the turnip accessions. Minor forms of GLS include gluconapoleiferin, glucobrassicanapin, glucoerucin, glucobrassin, and 4-hydroxyglucobrassin. Both species exhibited strong antioxidant activity, attributed to glucosinolates and phenolic compounds. The methanol extracts of Brassica rapa accessions were assessed against a panel of five Gram-negative bacteria (Enterobacter cloacae, Escherichia coli, Pseudomonas aeruginosa, Salmonella enterica subsp. enterica serovar, and Yersinia enterocolitica) and three Gram-positive bacteria (Bacillus cereus, Listeria monocytogenes, and Staphylococcus aureus). The extracts exhibited activity against S. enterica and S. aureus, and two showed inhibitory activity against E. coli and Y. enterocolitica. This study provides valuable insights into the chemical composition and biological properties of Brassica rapa and Brassica napus collections in Portugal. The selected accessions can constitute potential sources of natural antioxidants and bioactive compounds, which can be used in breeding programs and improving human health and to promote healthy food systems. |
| dirty | 0 |
| eu_rights_str_mv | openAccess |
| format | article |
| fulltext.url.fl_str_mv | https://repositorio.ulisboa.pt/bitstreams/ce642519-7d84-41a6-b04f-fa80ebdaa739/download |
| funder_facet_str_mv | FCT{{{_:::_}}}Fundação para a Ciência e a Tecnologia FCT{{{_:::_}}}Fundação para a Ciência e a Tecnologia |
| funding.funder.alternateName_str_mv | FCT FCT |
| funding.funder.identifier_str_mv | http://doi.org/10.13039/501100001871 http://doi.org/10.13039/501100001871 |
| funding.funder.name_str_mv | Fundação para a Ciência e a Tecnologia Fundação para a Ciência e a Tecnologia |
| funding.identifier_str_mv | UIDB/00690/2020 UIDP/00100/2020 |
| funding.name_str_mv | 6817 - DCRRNI ID 6817 - DCRRNI ID |
| funding_str_mv | UIDB/00690/2020 info:eu-repo/grantAgreement/FCT/6817 - DCRRNI ID/UIDB%2F00690%2F2020/PT UIDP/00100/2020 info:eu-repo/grantAgreement/FCT/6817 - DCRRNI ID/UIDP%2F00100%2F2020/PT |
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| identifier.url.fl_str_mv | http://hdl.handle.net/10400.5/102386 |
| inst_facet_str | urn:organizationAcronym:ul{{{_:::_}}}Universidade de Lisboa |
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| institution | Universidade de Lisboa |
| instname_str | Universidade de Lisboa |
| language | eng |
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| network_name_str | Repositório da Universidade de Lisboa |
| oai_identifier_str | oai:repositorio.ulisboa.pt:10400.5/102386 |
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| person_str_mv | Serrano, Carmo Serrano, Carmo https://www.ciencia-id.pt/5713-9F35-DCDF 5713-9F35-DCDF http://orcid.org/0000-0002-7632-3485 0000-0002-7632-3485 Oliveira, M. Conceição Lopes, V. R. Soares, Andreia Molina, Adriana K. Paschoalinotto, Beatriz H. Pires, Tânia C. S. P. Serra, Octávio Barata, Ana M. |
| publishDate | 2024 |
| publisher.none.fl_str_mv | MDPI |
| repo_facet_str | urn:repositoryAcronym:ul{{{_:::_}}}Repositório da Universidade de Lisboa |
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| spelling | engMDPIpt_PTThis study aimed to analyse the chemical profile and biological activities of 29 accessions of Brassica rapa (turnips) and 9 of Brassica napus (turnips and seeds) collections, maintained ex situ in Por- tugal. HPLC-HRMS allowed the determination of glucosinolates (GLS) and polyphenolic compounds. The antioxidant and antimicrobial activities were determined by using relevant assays. The chemical profiles showed that glucosamine, gluconasturtiin, and neoglucobrassin were the most abundant GLS in the extracts from the turnip accessions. Minor forms of GLS include gluconapoleiferin, glucobrassicanapin, glucoerucin, glucobrassin, and 4-hydroxyglucobrassin. Both species exhibited strong antioxidant activity, attributed to glucosinolates and phenolic compounds. The methanol extracts of Brassica rapa accessions were assessed against a panel of five Gram-negative bacteria (Enterobacter cloacae, Escherichia coli, Pseudomonas aeruginosa, Salmonella enterica subsp. enterica serovar, and Yersinia enterocolitica) and three Gram-positive bacteria (Bacillus cereus, Listeria monocytogenes, and Staphylococcus aureus). The extracts exhibited activity against S. enterica and S. aureus, and two showed inhibitory activity against E. coli and Y. enterocolitica. This study provides valuable insights into the chemical composition and biological properties of Brassica rapa and Brassica napus collections in Portugal. The selected accessions can constitute potential sources of natural antioxidants and bioactive compounds, which can be used in breeding programs and improving human health and to promote healthy food systems.application/pdfpt_PTChemical Profile and Biological Activities of Brassica rapa and Brassica napus Ex Situ Collection from PortugalPersonalSerrano, CarmoDSpacehttp://dspace.org/items/8bff06b6-a003-48de-875b-99402d068438DSpacehttp://dspace.org/items/8bff06b6-a003-48de-875b-99402d068438Martins SerranoMaria do CarmoCiência IDhttps://www.ciencia-id.pt5713-9F35-DCDFORCIDhttp://orcid.org0000-0002-7632-3485Scopus Author IDhttps://www.scopus.com41862528200Oliveira, M. ConceiçãoLopes, V. R.Soares, AndreiaMolina, Adriana K.Paschoalinotto, Beatriz H.Pires, Tânia C. S. P.Serra, OctávioBarata, Ana M.HostingInstitutionOrganizationalRepositório Científico de Acesso Aberto da ULisboae-mailmailto:repositorio@ulisboa.ptrepositorio@ulisboa.ptDOIIsPartOf10.3390/foods130811642025-07-23T15:08:36Z2024-042024-04-01T00:00:00ZHandlehttp://hdl.handle.net/10400.5/102386http://purl.org/coar/access_right/c_abf2open accessBrassica rapaBrassica napusglucosinolatesphenolic compoundsantibacterial activityantioxidant activity1685158 bytesFundação para a Ciência e a TecnologiaMountain Research Centerinfo:eu-repo/grantAgreement/FCT/6817 - DCRRNI ID/UIDB%2F00690%2F2020/PTUIDB/00690/20206817 - DCRRNI IDCrossref Funder IDhttp://doi.org/10.13039/501100001871Fundação para a Ciência e a TecnologiaCentro de Química Estruturalinfo:eu-repo/grantAgreement/FCT/6817 - DCRRNI ID/UIDP%2F00100%2F2020/PTUIDP/00100/20206817 - DCRRNI IDCrossref Funder IDhttp://doi.org/10.13039/501100001871literaturehttp://purl.org/coar/resource_type/c_6501journal article2024-04http://creativecommons.org/licenses/by/4.0/http://purl.org/coar/access_right/c_abf2application/pdffulltexthttps://repositorio.ulisboa.pt/bitstreams/ce642519-7d84-41a6-b04f-fa80ebdaa739/downloadFoods1381164 |
| spellingShingle | Chemical Profile and Biological Activities of Brassica rapa and Brassica napus Ex Situ Collection from Portugal Serrano, Carmo Brassica rapa Brassica napus glucosinolates phenolic compounds antibacterial activity antioxidant activity |
| status | SINGLETON |
| subject.fl_str_mv | Brassica rapa Brassica napus glucosinolates phenolic compounds antibacterial activity antioxidant activity |
| title | Chemical Profile and Biological Activities of Brassica rapa and Brassica napus Ex Situ Collection from Portugal |
| title_full | Chemical Profile and Biological Activities of Brassica rapa and Brassica napus Ex Situ Collection from Portugal |
| title_fullStr | Chemical Profile and Biological Activities of Brassica rapa and Brassica napus Ex Situ Collection from Portugal |
| title_full_unstemmed | Chemical Profile and Biological Activities of Brassica rapa and Brassica napus Ex Situ Collection from Portugal |
| title_short | Chemical Profile and Biological Activities of Brassica rapa and Brassica napus Ex Situ Collection from Portugal |
| title_sort | Chemical Profile and Biological Activities of Brassica rapa and Brassica napus Ex Situ Collection from Portugal |
| topic | Brassica rapa Brassica napus glucosinolates phenolic compounds antibacterial activity antioxidant activity |
| topic_facet | Brassica rapa Brassica napus glucosinolates phenolic compounds antibacterial activity antioxidant activity |
| url | http://hdl.handle.net/10400.5/102386 |
| visible | 1 |