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Effect of naringin enzymatic hydrolysis towards naringenin on the anti-inflammatory activity of both compounds
| Resumo: | The aim of this work was the hydrolysis of naringin towards naringenin with immobilized naringinase and the evaluation of their anti-inflammatory activity. An acute local inflammation model (rat paw edema induced by X-carrageenan) was chosen to evaluate the contribution of antioxidant properties to a possible anti-inflammatory effect. Grapefruit juice was processed with naringinase immobilized in k-carrageenan (2%) beads (approximate to 3 mm). A 95% naringin conversion in grapefruit juice was obtained with immobilized naringinase (1000 mg L-1), with an activity of 19.5 mg mL(-1) min(-1) and the formation of 215 mg L-1 of naringenin. Ascorbic acid and indometacine were used as positive anti-inflammatory controls. All results were analysed using ANOVA with Dunnett's Post-test. The results show that rats (n = 9) pre-treated with a solution of naringin, rats (n = 9) pre-treated with a solution of naringenin (in concentrations equal as in grapefruit juice) and rats (n = 9) treated with a solution of naringenin and naringin, revealed a significant reduction on edema formation, 6h after X-carrageenan injection. Naringenin demonstrated a high in vivo anti-inflammatory activity, only 8% of paw edema (p0.001) was observed in rats pre-treated with a solution of naringenin. Comparability studies, in rats administered orally with grapefruit juice (before and after processing), showed that enzymatic processing did not affect the anti-inflammatory properties of the juice. (C) 2007 Elsevier B.V. All rights reserved. |
|---|---|
| Autores principais: | Ribeiro, Isabel A. |
| Outros Autores: | Rocha, Joao; Sepodes, Bruno; Mota-Filipe, Helder; Ribeiro, Maria H. |
| Assunto: | Biochemistry & Molecular Biology Chemistry, Physical |
| Ano: | 2008 |
| País: | Portugal |
| Tipo de documento: | artigo |
| Tipo de acesso: | acesso a metadados |
| Instituição associada: | Universidade de Lisboa |
| Idioma: | inglês |
| Origem: | Repositório da Universidade de Lisboa |
| _version_ | 1877770806533029888 |
|---|---|
| author | Ribeiro, Isabel A. |
| author2 | Rocha, Joao Sepodes, Bruno Mota-Filipe, Helder Ribeiro, Maria H. |
| author2_role | author author author author |
| author_facet | Ribeiro, Isabel A. Rocha, Joao Sepodes, Bruno Mota-Filipe, Helder Ribeiro, Maria H. |
| author_role | author |
| contributor_name_str_mv | Repositório Científico de Acesso Aberto da ULisboa |
| country_str | PT |
| creators_json_txt | [{\"Person.name\":\"Ribeiro, Isabel A.\"},{\"Person.name\":\"Rocha, Joao\"},{\"Person.name\":\"Sepodes, Bruno\"},{\"Person.name\":\"Mota-Filipe, Helder\"},{\"Person.name\":\"Ribeiro, Maria H.\"}] |
| datacite.contributors.contributor.contributorName.fl_str_mv | Repositório Científico de Acesso Aberto da ULisboa |
| datacite.creators.creator.creatorName.fl_str_mv | Ribeiro, Isabel A. Rocha, Joao Sepodes, Bruno Mota-Filipe, Helder Ribeiro, Maria H. |
| datacite.date.Accepted.fl_str_mv | 2008-01-01T00:00:00Z |
| datacite.date.available.fl_str_mv | 2015-12-30T10:17:17Z |
| datacite.date.embargoed.fl_str_mv | 2015-12-30T10:17:17Z |
| datacite.rights.fl_str_mv | http://purl.org/coar/access_right/c_14cb |
| datacite.subjects.subject.fl_str_mv | Biochemistry & Molecular Biology Chemistry, Physical |
| datacite.titles.title.fl_str_mv | Effect of naringin enzymatic hydrolysis towards naringenin on the anti-inflammatory activity of both compounds |
| dc.contributor.none.fl_str_mv | Repositório Científico de Acesso Aberto da ULisboa |
| dc.creator.none.fl_str_mv | Ribeiro, Isabel A. Rocha, Joao Sepodes, Bruno Mota-Filipe, Helder Ribeiro, Maria H. |
| dc.date.Accepted.fl_str_mv | 2008-01-01T00:00:00Z |
| dc.date.available.fl_str_mv | 2015-12-30T10:17:17Z |
| dc.date.embargoed.fl_str_mv | 2015-12-30T10:17:17Z |
| dc.identifier.none.fl_str_mv | http://hdl.handle.net/10451/20993 |
| dc.language.none.fl_str_mv | eng |
| dc.publisher.none.fl_str_mv | ELSEVIER SCIENCE BV |
| dc.rights.none.fl_str_mv | http://purl.org/coar/access_right/c_14cb |
| dc.subject.none.fl_str_mv | Biochemistry & Molecular Biology Chemistry, Physical |
| dc.title.fl_str_mv | Effect of naringin enzymatic hydrolysis towards naringenin on the anti-inflammatory activity of both compounds |
| dc.type.none.fl_str_mv | http://purl.org/coar/resource_type/c_6501 |
| description | The aim of this work was the hydrolysis of naringin towards naringenin with immobilized naringinase and the evaluation of their anti-inflammatory activity. An acute local inflammation model (rat paw edema induced by X-carrageenan) was chosen to evaluate the contribution of antioxidant properties to a possible anti-inflammatory effect. Grapefruit juice was processed with naringinase immobilized in k-carrageenan (2%) beads (approximate to 3 mm). A 95% naringin conversion in grapefruit juice was obtained with immobilized naringinase (1000 mg L-1), with an activity of 19.5 mg mL(-1) min(-1) and the formation of 215 mg L-1 of naringenin. Ascorbic acid and indometacine were used as positive anti-inflammatory controls. All results were analysed using ANOVA with Dunnett's Post-test. The results show that rats (n = 9) pre-treated with a solution of naringin, rats (n = 9) pre-treated with a solution of naringenin (in concentrations equal as in grapefruit juice) and rats (n = 9) treated with a solution of naringenin and naringin, revealed a significant reduction on edema formation, 6h after X-carrageenan injection. Naringenin demonstrated a high in vivo anti-inflammatory activity, only 8% of paw edema (p0.001) was observed in rats pre-treated with a solution of naringenin. Comparability studies, in rats administered orally with grapefruit juice (before and after processing), showed that enzymatic processing did not affect the anti-inflammatory properties of the juice. (C) 2007 Elsevier B.V. All rights reserved. |
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| format | article |
| id | ul_1440bef7d8eae16d2bd33a96cd9cd609 |
| identifier.url.fl_str_mv | http://hdl.handle.net/10451/20993 |
| inst_facet_str | urn:organizationAcronym:ul{{{_:::_}}}Universidade de Lisboa |
| instacron_str | ul |
| institution | Universidade de Lisboa |
| instname_str | Universidade de Lisboa |
| language | eng |
| network_acronym_str | ul |
| network_name_str | Repositório da Universidade de Lisboa |
| oai_identifier_str | oai:repositorio.ulisboa.pt:10451/20993 |
| organization_str_mv | urn:organizationAcronym:ul |
| person_str_mv | Ribeiro, Isabel A. Rocha, Joao Sepodes, Bruno Mota-Filipe, Helder Ribeiro, Maria H. |
| publishDate | 2008 |
| publisher.none.fl_str_mv | ELSEVIER SCIENCE BV |
| repo_facet_str | urn:repositoryAcronym:ul{{{_:::_}}}Repositório da Universidade de Lisboa |
| reponame_str | Repositório da Universidade de Lisboa |
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| spelling | engELSEVIER SCIENCE BVThe aim of this work was the hydrolysis of naringin towards naringenin with immobilized naringinase and the evaluation of their anti-inflammatory activity. An acute local inflammation model (rat paw edema induced by X-carrageenan) was chosen to evaluate the contribution of antioxidant properties to a possible anti-inflammatory effect. Grapefruit juice was processed with naringinase immobilized in k-carrageenan (2%) beads (approximate to 3 mm). A 95% naringin conversion in grapefruit juice was obtained with immobilized naringinase (1000 mg L-1), with an activity of 19.5 mg mL(-1) min(-1) and the formation of 215 mg L-1 of naringenin. Ascorbic acid and indometacine were used as positive anti-inflammatory controls. All results were analysed using ANOVA with Dunnett's Post-test. The results show that rats (n = 9) pre-treated with a solution of naringin, rats (n = 9) pre-treated with a solution of naringenin (in concentrations equal as in grapefruit juice) and rats (n = 9) treated with a solution of naringenin and naringin, revealed a significant reduction on edema formation, 6h after X-carrageenan injection. Naringenin demonstrated a high in vivo anti-inflammatory activity, only 8% of paw edema (p0.001) was observed in rats pre-treated with a solution of naringenin. Comparability studies, in rats administered orally with grapefruit juice (before and after processing), showed that enzymatic processing did not affect the anti-inflammatory properties of the juice. (C) 2007 Elsevier B.V. All rights reserved.Effect of naringin enzymatic hydrolysis towards naringenin on the anti-inflammatory activity of both compoundsRibeiro, Isabel A.Rocha, JoaoSepodes, BrunoMota-Filipe, HelderRibeiro, Maria H.HostingInstitutionOrganizationalRepositório Científico de Acesso Aberto da ULisboae-mailmailto:repositorio@ulisboa.ptrepositorio@ulisboa.ptISSNIsPartOf1381-1177DOIIsPartOfhttp://dx.doi.org/10.1016/j.molcatb.2007.10.0112015-12-30T10:17:17Z20082008-01-01T00:00:00ZHandlehttp://hdl.handle.net/10451/20993http://purl.org/coar/access_right/c_14cbmetadata only accessBiochemistry & Molecular BiologyChemistry, Physicalliteraturehttp://purl.org/coar/resource_type/c_6501journal articleJOURNAL OF MOLECULAR CATALYSIS B-ENZYMATICVol. 52-3 (JUN 2008)1318 |
| spellingShingle | Effect of naringin enzymatic hydrolysis towards naringenin on the anti-inflammatory activity of both compounds Ribeiro, Isabel A. Biochemistry & Molecular Biology Chemistry, Physical |
| status | SINGLETON |
| subject.fl_str_mv | Biochemistry & Molecular Biology Chemistry, Physical |
| title | Effect of naringin enzymatic hydrolysis towards naringenin on the anti-inflammatory activity of both compounds |
| title_full | Effect of naringin enzymatic hydrolysis towards naringenin on the anti-inflammatory activity of both compounds |
| title_fullStr | Effect of naringin enzymatic hydrolysis towards naringenin on the anti-inflammatory activity of both compounds |
| title_full_unstemmed | Effect of naringin enzymatic hydrolysis towards naringenin on the anti-inflammatory activity of both compounds |
| title_short | Effect of naringin enzymatic hydrolysis towards naringenin on the anti-inflammatory activity of both compounds |
| title_sort | Effect of naringin enzymatic hydrolysis towards naringenin on the anti-inflammatory activity of both compounds |
| topic | Biochemistry & Molecular Biology Chemistry, Physical |
| topic_facet | Biochemistry & Molecular Biology Chemistry, Physical |
| url | http://hdl.handle.net/10451/20993 |
| visible | 1 |
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