Publication
Ability of the MSC secretome to repair synaptic structure and function after hypoxia-induced injury
| Summary: | Ischemic stroke (IS) remains one of the leading causes of death and disability worldwide, representing a major clinical and economic challenge. It is caused by the temporary or permanent interruption of a blood vessel, leading to disruption of the central nervous system and consequent neuronal loss. In the acute phase, therapeutic strategies include thrombolysis and thrombectomy to restore blood flow, whereas in the chronic phase, approaches aimed at promoting neural plasticity, such as cell therapy, are employed. However, these techniques face several limitations, as their efficacy is directly influenced by multiple variables, making their response unpredictable. Recent studies have attributed the effects of mesenchymal stem cells (MSCs) mainly to their paracrine action, thus positioning the use of their secretome as a promising alternative. In this work, we evaluated the impact of secretome derived from umbilical cord MSCs (UC-MSCs), obtained under normoxic and hypoxic conditions, on neuronal viability and on the recovery of synaptic structure after oxygen and glucose deprivation (OGD) in vitro. The results suggest that the secretome can modulate cell survival, preserve the length of neuronal arborization processes, and modulate synaptic markers. Although some results are promising, further studies are required to explore alternative approaches in order to better understand the action of MSC-derived secretome in the treatment of ischemic stroke and other neurological disorders associated with hypoxicischemic injury. |
|---|---|
| Main Authors: | Marceta, Bragança Lourenço Moisés |
| Subject: | Acidente vascular cerebral isquémico Células estaminais mesenquimais Secretoma Hipóxia Neuroproteção Sinaptogénese Ischemic stroke Mesenchymal stem cells Secretome Hypoxia Neuroprotection Synaptogenesis |
| Year: | 2025 |
| Country: | Portugal |
| Document type: | master thesis |
| Access type: | embargoed access |
| Associated institution: | Universidade da Beira Interior |
| Language: | English |
| Origin: | uBibliorum |
| _version_ | 1868421017546784768 |
|---|---|
| author | Marceta, Bragança Lourenço Moisés |
| author_facet | Marceta, Bragança Lourenço Moisés |
| author_role | author |
| contributor_name_str_mv | Baltazar, Graça Maria Fernandes uBibliorum |
| country_str | PT |
| creators_json_txt | [{\"Person.name\":\"Marceta, Bragança Lourenço Moisés\",\"Person.identifier.orcid\":\"0009-0009-3456-6655\"}] |
| datacite.contributors.contributor.contributorName.fl_str_mv | Baltazar, Graça Maria Fernandes uBibliorum |
| datacite.creators.creator.creatorName.fl_str_mv | Marceta, Bragança Lourenço Moisés |
| datacite.date.Accepted.fl_str_mv | 2025-11-19T00:00:00Z |
| datacite.date.available.fl_str_mv | 2026-01-22T15:29:19Z |
| datacite.date.embargoed.fl_str_mv | 2026-01-22T15:29:19Z |
| datacite.rights.fl_str_mv | http://purl.org/coar/access_right/c_f1cf |
| datacite.subjects.subject.fl_str_mv | Acidente vascular cerebral isquémico Células estaminais mesenquimais Secretoma Hipóxia Neuroproteção Sinaptogénese Ischemic stroke Mesenchymal stem cells Secretome Hypoxia Neuroprotection Synaptogenesis |
| datacite.titles.title.fl_str_mv | Ability of the MSC secretome to repair synaptic structure and function after hypoxia-induced injury |
| dc.contributor.none.fl_str_mv | Baltazar, Graça Maria Fernandes uBibliorum |
| dc.creator.none.fl_str_mv | Marceta, Bragança Lourenço Moisés |
| dc.date.Accepted.fl_str_mv | 2025-11-19T00:00:00Z |
| dc.date.available.fl_str_mv | 2026-01-22T15:29:19Z |
| dc.date.embargoed.fl_str_mv | 2026-01-22T15:29:19Z |
| dc.description.none.fl_str_mv | O acidente vascular cerebral isquémico (IS, do inglês Ischemic Stroke) continua a representar uma das principais causas de morte e incapacidade no mundo, representando um desafio clínico e económico. É causado pela interrupção temporária ou permanente de um vaso sanguíneo causando disrupção a nível do sistema nervoso central devida à morte neural. Em fase aguda as estratégias incluem o uso de técnicas como trombólise e tromboctomia como forma de restaurar o fluxo sanguíneo. Em fase crónica são empregues técnicas que visam promover a plasticidade neural tais como a terapia celular, porém o uso dessas abordagens é influenciada por múltiplas variáveis, o que ainda limita o seu uso. Estudos recentes têm atribuído os efeitos das células estaminais mesenquimais (MSCs) à sua ação parácrina, emergindo assim o uso do seu secretoma como uma alternativa promissora. Neste trabalho, avaliou-se o impacto do secretoma derivado de MSCs do cordão umbilical (UC-MSCs), obtido sob condições normóxicas e hipóxicas, sobre a viabilidade neuronal e a recuperação da estrutura sináptica após lesão induzida por privação de oxigénio e glicose (OGD) in vitro. Os resultados sugerem que o secretoma pode modular a sobrevivência celular, preservar o comprimento de prolongamentos da arborização neuronal e modular marcadores sinápticos. Embora alguns resultados sejam promissores, é necessário, explorar outras abordagens como forma de melhor compreender a ação do secretoma das MSCs no tratamento do AVC isquémico e em outras patologias neurológicas associadas a lesão hipóxicoisquémica. |
| dc.format.none.fl_str_mv | application/pdf |
| dc.identifier.none.fl_str_mv | http://hdl.handle.net/10400.6/19747 |
| dc.language.none.fl_str_mv | eng |
| dc.rights.cclincense.fl_str_mv | http://creativecommons.org/licenses/by-nc-sa/4.0/ |
| dc.rights.none.fl_str_mv | http://purl.org/coar/access_right/c_f1cf |
| dc.subject.none.fl_str_mv | Acidente vascular cerebral isquémico Células estaminais mesenquimais Secretoma Hipóxia Neuroproteção Sinaptogénese Ischemic stroke Mesenchymal stem cells Secretome Hypoxia Neuroprotection Synaptogenesis |
| dc.title.fl_str_mv | Ability of the MSC secretome to repair synaptic structure and function after hypoxia-induced injury |
| dc.type.none.fl_str_mv | http://purl.org/coar/resource_type/c_bdcc |
| description | Ischemic stroke (IS) remains one of the leading causes of death and disability worldwide, representing a major clinical and economic challenge. It is caused by the temporary or permanent interruption of a blood vessel, leading to disruption of the central nervous system and consequent neuronal loss. In the acute phase, therapeutic strategies include thrombolysis and thrombectomy to restore blood flow, whereas in the chronic phase, approaches aimed at promoting neural plasticity, such as cell therapy, are employed. However, these techniques face several limitations, as their efficacy is directly influenced by multiple variables, making their response unpredictable. Recent studies have attributed the effects of mesenchymal stem cells (MSCs) mainly to their paracrine action, thus positioning the use of their secretome as a promising alternative. In this work, we evaluated the impact of secretome derived from umbilical cord MSCs (UC-MSCs), obtained under normoxic and hypoxic conditions, on neuronal viability and on the recovery of synaptic structure after oxygen and glucose deprivation (OGD) in vitro. The results suggest that the secretome can modulate cell survival, preserve the length of neuronal arborization processes, and modulate synaptic markers. Although some results are promising, further studies are required to explore alternative approaches in order to better understand the action of MSC-derived secretome in the treatment of ischemic stroke and other neurological disorders associated with hypoxicischemic injury. |
| dirty | 0 |
| eu_rights_str_mv | embargoedAccess |
| format | masterThesis |
| fulltext.url.fl_str_mv | https://ubibliorum.ubi.pt/bitstreams/c7ec2757-f82e-4f85-84c2-7398b9da7931/download |
| funding.identifier_str_mv | PL23-00001 POCI-01-0145-FEDER-022122 |
| funding_str_mv | PL23-00001 https://cnc.uc.pt/pt/rd-projects/repair-reparar-e-recuperar-no-avc-isquemico-novas-estrategias-de-terapia-celularhttp://hdl.handle.net/10400.6/19745 POCI-01-0145-FEDER-022122 https://transparencia.gov.pt/pt/fundos-europeus/pt2020/beneficiarios-projetos/projeto/POCI-01-0145-FEDER-022122/#project_information_idhttp://hdl.handle.net/10400.6/19746 |
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| identifier.url.fl_str_mv | http://hdl.handle.net/10400.6/19747 |
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| institution | Universidade da Beira Interior |
| instname_str | Universidade da Beira Interior |
| language | eng |
| network_acronym_str | ubibliorum |
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| oai_identifier_str | oai:ubibliorum.ubi.pt:10400.6/19747 |
| organization_str_mv | urn:organizationAcronym:ubi |
| person_str_mv | Marceta, Bragança Lourenço Moisés Marceta, Bragança Lourenço Moisés https://www.ciencia-id.pt/BE1B-5B98-ED75 BE1B-5B98-ED75 http://orcid.org/0009-0009-3456-6655 0009-0009-3456-6655 |
| publishDate | 2025 |
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| service_str_mv | urn:repositoryAcronym:ubibliorum |
| spelling | engengIschemic stroke (IS) remains one of the leading causes of death and disability worldwide, representing a major clinical and economic challenge. It is caused by the temporary or permanent interruption of a blood vessel, leading to disruption of the central nervous system and consequent neuronal loss. In the acute phase, therapeutic strategies include thrombolysis and thrombectomy to restore blood flow, whereas in the chronic phase, approaches aimed at promoting neural plasticity, such as cell therapy, are employed. However, these techniques face several limitations, as their efficacy is directly influenced by multiple variables, making their response unpredictable. Recent studies have attributed the effects of mesenchymal stem cells (MSCs) mainly to their paracrine action, thus positioning the use of their secretome as a promising alternative. In this work, we evaluated the impact of secretome derived from umbilical cord MSCs (UC-MSCs), obtained under normoxic and hypoxic conditions, on neuronal viability and on the recovery of synaptic structure after oxygen and glucose deprivation (OGD) in vitro. The results suggest that the secretome can modulate cell survival, preserve the length of neuronal arborization processes, and modulate synaptic markers. Although some results are promising, further studies are required to explore alternative approaches in order to better understand the action of MSC-derived secretome in the treatment of ischemic stroke and other neurological disorders associated with hypoxicischemic injury.porO acidente vascular cerebral isquémico (IS, do inglês Ischemic Stroke) continua a representar uma das principais causas de morte e incapacidade no mundo, representando um desafio clínico e económico. É causado pela interrupção temporária ou permanente de um vaso sanguíneo causando disrupção a nível do sistema nervoso central devida à morte neural. Em fase aguda as estratégias incluem o uso de técnicas como trombólise e tromboctomia como forma de restaurar o fluxo sanguíneo. Em fase crónica são empregues técnicas que visam promover a plasticidade neural tais como a terapia celular, porém o uso dessas abordagens é influenciada por múltiplas variáveis, o que ainda limita o seu uso. Estudos recentes têm atribuído os efeitos das células estaminais mesenquimais (MSCs) à sua ação parácrina, emergindo assim o uso do seu secretoma como uma alternativa promissora. Neste trabalho, avaliou-se o impacto do secretoma derivado de MSCs do cordão umbilical (UC-MSCs), obtido sob condições normóxicas e hipóxicas, sobre a viabilidade neuronal e a recuperação da estrutura sináptica após lesão induzida por privação de oxigénio e glicose (OGD) in vitro. Os resultados sugerem que o secretoma pode modular a sobrevivência celular, preservar o comprimento de prolongamentos da arborização neuronal e modular marcadores sinápticos. Embora alguns resultados sejam promissores, é necessário, explorar outras abordagens como forma de melhor compreender a ação do secretoma das MSCs no tratamento do AVC isquémico e em outras patologias neurológicas associadas a lesão hipóxicoisquémica.application/pdfporAbility of the MSC secretome to repair synaptic structure and function after hypoxia-induced injuryPersonalMarceta, Bragança Lourenço MoisésDSpacehttp://dspace.org/items/1aee4e7c-f38b-457d-90fe-af04ea907b5fDSpacehttp://dspace.org/items/1aee4e7c-f38b-457d-90fe-af04ea907b5fMarcetaBragança Lourenço MoisésCiência IDhttps://www.ciencia-id.ptBE1B-5B98-ED75ORCIDhttp://orcid.org0009-0009-3456-6655Baltazar, Graça Maria FernandesHostingInstitutionOrganizationaluBibliorume-mailmailto:noreplyuBibliorum@ubi.ptnoreplyuBibliorum@ubi.ptURNurn:tid:2041371442026-01-22T15:29:19Z2025-11-192025-11-19T00:00:00ZHandlehttp://hdl.handle.net/10400.6/19747http://purl.org/coar/access_right/c_f1cfembargoed accessAcidente vascular cerebral isquémicoCélulas estaminais mesenquimaisSecretomaHipóxiaNeuroproteçãoSinaptogéneseIschemic strokeMesenchymal stem cellsSecretomeHypoxiaNeuroprotectionSynaptogenesis2948991 bytesREPAIR - Reparar e Recuperar no AVC isquémico: novas estratégias de terapia celular [PL23-00001]https://cnc.uc.pt/pt/rd-projects/repair-reparar-e-recuperar-no-avc-isquemico-novas-estrategias-de-terapia-celularhttp://hdl.handle.net/10400.6/19745PL23-00001PPBI: Plataforma Portuguesa de BioImagem [POCI-01-0145-FEDER-022122]https://transparencia.gov.pt/pt/fundos-europeus/pt2020/beneficiarios-projetos/projeto/POCI-01-0145-FEDER-022122/#project_information_idhttp://hdl.handle.net/10400.6/19746POCI-01-0145-FEDER-022122literaturehttp://purl.org/coar/resource_type/c_bdccmaster thesis2025-11-19http://creativecommons.org/licenses/by-nc-sa/4.0/http://purl.org/coar/access_right/c_f1cfapplication/pdffulltexthttps://ubibliorum.ubi.pt/bitstreams/c7ec2757-f82e-4f85-84c2-7398b9da7931/download |
| spellingShingle | Ability of the MSC secretome to repair synaptic structure and function after hypoxia-induced injury Marceta, Bragança Lourenço Moisés Acidente vascular cerebral isquémico Células estaminais mesenquimais Secretoma Hipóxia Neuroproteção Sinaptogénese Ischemic stroke Mesenchymal stem cells Secretome Hypoxia Neuroprotection Synaptogenesis |
| status | SINGLETON |
| subject.fl_str_mv | Acidente vascular cerebral isquémico Células estaminais mesenquimais Secretoma Hipóxia Neuroproteção Sinaptogénese Ischemic stroke Mesenchymal stem cells Secretome Hypoxia Neuroprotection Synaptogenesis |
| title | Ability of the MSC secretome to repair synaptic structure and function after hypoxia-induced injury |
| title_full | Ability of the MSC secretome to repair synaptic structure and function after hypoxia-induced injury |
| title_fullStr | Ability of the MSC secretome to repair synaptic structure and function after hypoxia-induced injury |
| title_full_unstemmed | Ability of the MSC secretome to repair synaptic structure and function after hypoxia-induced injury |
| title_short | Ability of the MSC secretome to repair synaptic structure and function after hypoxia-induced injury |
| title_sort | Ability of the MSC secretome to repair synaptic structure and function after hypoxia-induced injury |
| topic | Acidente vascular cerebral isquémico Células estaminais mesenquimais Secretoma Hipóxia Neuroproteção Sinaptogénese Ischemic stroke Mesenchymal stem cells Secretome Hypoxia Neuroprotection Synaptogenesis |
| topic_facet | Acidente vascular cerebral isquémico Células estaminais mesenquimais Secretoma Hipóxia Neuroproteção Sinaptogénese Ischemic stroke Mesenchymal stem cells Secretome Hypoxia Neuroprotection Synaptogenesis |
| url | http://hdl.handle.net/10400.6/19747 |
| visible | 1 |