Publicação
Optimization of liquid overlay technique to formulate heterogenic 3D co‐cultures models
| Resumo: | Three‐dimensional (3D) cell culture models of solid tumors are currently having a tremendous impact in the in vitro screening of candidate anti‐tumoral therapies. These 3D models provide more reliable results than those provided by standard 2D in vitro cell cultures. However, 3D manufacturing techniques need to be further optimized in order to increase the robustness of these models and provide data that can be properly correlated with the in vivo situation. Therefore, in the present study the parameters used for producing multicellular tumor spheroids (MCTS) by liquid overlay technique (LOT) were optimized in order to produce heterogeneous cellular agglomerates comprised of cancer cells and stromal cells, during long periods. Spheroids were produced under highly controlled conditions, namely: (i) agarose coatings; (ii) horizontal stirring, and (iii) a known initial cell number. The simultaneous optimization of these parameters promoted the assembly of 3D characteristic cellular organization similar to that found in the in vivo solid tumors. Such improvements in the LOT technique promoted the assembly of highly reproducible, individual 3D spheroids, with a low cost of production and that can be used for future in vitro drug screening assays. |
|---|---|
| Autores principais: | Costa, Elisabete C. |
| Outros Autores: | Gaspar, Vítor Manuel Abreu; Coutinho, Paula; Correia, Ilídio Joaquim Sobreira |
| Assunto: | Cancer In vitro models LOT 3D MCTS Tumor microenvironment |
| Ano: | 2014 |
| País: | Portugal |
| Tipo de documento: | artigo |
| Tipo de acesso: | acesso restrito |
| Instituição associada: | Universidade da Beira Interior |
| Idioma: | inglês |
| Origem: | uBibliorum |
| _version_ | 1868420707691528192 |
|---|---|
| author | Costa, Elisabete C. |
| author2 | Gaspar, Vítor Manuel Abreu Coutinho, Paula Correia, Ilídio Joaquim Sobreira |
| author2_role | author author author |
| author_facet | Costa, Elisabete C. Gaspar, Vítor Manuel Abreu Coutinho, Paula Correia, Ilídio Joaquim Sobreira |
| author_role | author |
| contributor_name_str_mv | uBibliorum |
| country_str | PT |
| creators_json_txt | [{\"Person.name\":\"Costa, Elisabete C.\",\"Person.identifier.orcid\":\"0000-0002-0490-0095\"},{\"Person.name\":\"Gaspar, Vítor Manuel Abreu\",\"Person.identifier.orcid\":\"0000-0002-0372-2493\"},{\"Person.name\":\"Coutinho, Paula\",\"Person.identifier.orcid\":\"0000-0001-6832-3082\"},{\"Person.name\":\"Correia, Ilídio Joaquim Sobreira\",\"Person.identifier.orcid\":\"0000-0003-1613-9675\"}] |
| datacite.contributors.contributor.contributorName.fl_str_mv | uBibliorum |
| datacite.creators.creator.creatorName.fl_str_mv | Costa, Elisabete C. Gaspar, Vítor Manuel Abreu Coutinho, Paula Correia, Ilídio Joaquim Sobreira |
| datacite.date.Accepted.fl_str_mv | 2014-02-25T00:00:00Z |
| datacite.date.available.fl_str_mv | 2018-03-20T10:38:37Z |
| datacite.date.embargoed.fl_str_mv | 2018-03-20T10:38:37Z |
| datacite.rights.fl_str_mv | http://purl.org/coar/access_right/c_16ec |
| datacite.subjects.subject.fl_str_mv | Cancer In vitro models LOT 3D MCTS Tumor microenvironment |
| datacite.titles.title.fl_str_mv | Optimization of liquid overlay technique to formulate heterogenic 3D co‐cultures models |
| dc.contributor.none.fl_str_mv | uBibliorum |
| dc.creator.none.fl_str_mv | Costa, Elisabete C. Gaspar, Vítor Manuel Abreu Coutinho, Paula Correia, Ilídio Joaquim Sobreira |
| dc.date.Accepted.fl_str_mv | 2014-02-25T00:00:00Z |
| dc.date.available.fl_str_mv | 2018-03-20T10:38:37Z |
| dc.date.embargoed.fl_str_mv | 2018-03-20T10:38:37Z |
| dc.format.none.fl_str_mv | application/pdf |
| dc.identifier.none.fl_str_mv | http://hdl.handle.net/10400.6/4650 |
| dc.language.none.fl_str_mv | eng |
| dc.publisher.none.fl_str_mv | Wiley |
| dc.rights.none.fl_str_mv | http://purl.org/coar/access_right/c_16ec |
| dc.subject.none.fl_str_mv | Cancer In vitro models LOT 3D MCTS Tumor microenvironment |
| dc.title.fl_str_mv | Optimization of liquid overlay technique to formulate heterogenic 3D co‐cultures models |
| dc.type.none.fl_str_mv | http://purl.org/coar/resource_type/c_6501 |
| description | Three‐dimensional (3D) cell culture models of solid tumors are currently having a tremendous impact in the in vitro screening of candidate anti‐tumoral therapies. These 3D models provide more reliable results than those provided by standard 2D in vitro cell cultures. However, 3D manufacturing techniques need to be further optimized in order to increase the robustness of these models and provide data that can be properly correlated with the in vivo situation. Therefore, in the present study the parameters used for producing multicellular tumor spheroids (MCTS) by liquid overlay technique (LOT) were optimized in order to produce heterogeneous cellular agglomerates comprised of cancer cells and stromal cells, during long periods. Spheroids were produced under highly controlled conditions, namely: (i) agarose coatings; (ii) horizontal stirring, and (iii) a known initial cell number. The simultaneous optimization of these parameters promoted the assembly of 3D characteristic cellular organization similar to that found in the in vivo solid tumors. Such improvements in the LOT technique promoted the assembly of highly reproducible, individual 3D spheroids, with a low cost of production and that can be used for future in vitro drug screening assays. |
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| eu_rights_str_mv | restrictedAccess |
| format | article |
| fulltext.url.fl_str_mv | https://ubibliorum.ubi.pt/bitstreams/6e29c2da-bdbb-40b1-8b29-1fecad6cdaef/download |
| funding.funder.alternateName_str_mv | FCT |
| funding.funder.identifier_str_mv | http://doi.org/10.13039/501100001871 |
| funding.funder.name_str_mv | Fundação para a Ciência e a Tecnologia |
| funding.identifier_str_mv | SFRH/BD/80402/2011 |
| funding.name_str_mv | FARH Bolsa de Doutoramento |
| funding_str_mv | SFRH/BD/80402/2011 info:eu-repo/grantAgreement/FCT/FARH/SFRH%2FBD%2F80402%2F2011/PT |
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| identifier.url.fl_str_mv | http://hdl.handle.net/10400.6/4650 |
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| institution | Universidade da Beira Interior |
| instname_str | Universidade da Beira Interior |
| language | eng |
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| person_str_mv | Costa, Elisabete C. Costa, Elisabete C. https://www.ciencia-id.pt/B314-4CF0-6633 B314-4CF0-6633 http://orcid.org/0000-0002-0490-0095 0000-0002-0490-0095 Gaspar, Vítor Manuel Abreu Gaspar, Vítor Manuel Abreu https://www.ciencia-id.pt/6F16-3640-73E3 6F16-3640-73E3 http://orcid.org/0000-0002-0372-2493 0000-0002-0372-2493 Coutinho, Paula Coutinho, Paula https://www.ciencia-id.pt/2311-7DBC-FA07 2311-7DBC-FA07 http://orcid.org/0000-0001-6832-3082 0000-0001-6832-3082 Correia, Ilídio Joaquim Sobreira Correia, Ilídio Joaquim Sobreira https://www.ciencia-id.pt/F610-7373-DC81 F610-7373-DC81 http://orcid.org/0000-0003-1613-9675 0000-0003-1613-9675 |
| publishDate | 2014 |
| publisher.none.fl_str_mv | Wiley |
| reponame_str | uBibliorum |
| repository_id_str | urn:repositoryAcronym:ubibliorum |
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| spelling | engWileypt_PTThree‐dimensional (3D) cell culture models of solid tumors are currently having a tremendous impact in the in vitro screening of candidate anti‐tumoral therapies. These 3D models provide more reliable results than those provided by standard 2D in vitro cell cultures. However, 3D manufacturing techniques need to be further optimized in order to increase the robustness of these models and provide data that can be properly correlated with the in vivo situation. Therefore, in the present study the parameters used for producing multicellular tumor spheroids (MCTS) by liquid overlay technique (LOT) were optimized in order to produce heterogeneous cellular agglomerates comprised of cancer cells and stromal cells, during long periods. Spheroids were produced under highly controlled conditions, namely: (i) agarose coatings; (ii) horizontal stirring, and (iii) a known initial cell number. The simultaneous optimization of these parameters promoted the assembly of 3D characteristic cellular organization similar to that found in the in vivo solid tumors. Such improvements in the LOT technique promoted the assembly of highly reproducible, individual 3D spheroids, with a low cost of production and that can be used for future in vitro drug screening assays.application/pdfpt_PTOptimization of liquid overlay technique to formulate heterogenic 3D co‐cultures modelsPersonalCosta, Elisabete C.DSpacehttp://dspace.org/items/bfc28f1b-bd1b-4564-97e3-ae6a0c3b6c0cDSpacehttp://dspace.org/items/bfc28f1b-bd1b-4564-97e3-ae6a0c3b6c0cda Rocha CostaElisabete CristinaCiência IDhttps://www.ciencia-id.ptB314-4CF0-6633ORCIDhttp://orcid.org0000-0002-0490-0095Scopus Author IDhttps://www.scopus.com55805850100PersonalGaspar, Vítor Manuel AbreuDSpacehttp://dspace.org/items/3eeac15e-ebf4-4dc2-aeb8-698087d6d2fcDSpacehttp://dspace.org/items/3eeac15e-ebf4-4dc2-aeb8-698087d6d2fcGasparVítorCiência IDhttps://www.ciencia-id.pt6F16-3640-73E3ORCIDhttp://orcid.org0000-0002-0372-2493Researcher IDhttps://www.researcherid.comB-1602-2017Scopus Author IDhttps://www.scopus.com36968590900PersonalCoutinho, PaulaDSpacehttp://dspace.org/items/33a4d54f-73df-4170-9244-498a68c255c1DSpacehttp://dspace.org/items/33a4d54f-73df-4170-9244-498a68c255c1BorgesPaula Isabel Teixeira Gonçalves CoutinhoCiência IDhttps://www.ciencia-id.pt2311-7DBC-FA07ORCIDhttp://orcid.org0000-0001-6832-3082Scopus Author IDhttps://www.scopus.com36709479900PersonalCorreia, Ilídio Joaquim SobreiraDSpacehttp://dspace.org/items/f2c3e779-34a2-4309-950a-80687664c6adDSpacehttp://dspace.org/items/f2c3e779-34a2-4309-950a-80687664c6adCorreiaIlídio Joaquim SobreiraCiência IDhttps://www.ciencia-id.ptF610-7373-DC81ORCIDhttp://orcid.org0000-0003-1613-9675Scopus Author IDhttps://www.scopus.com7003557499HostingInstitutionOrganizationaluBibliorume-mailmailto:noreplyuBibliorum@ubi.ptnoreplyuBibliorum@ubi.ptDOIIsPartOf10.1002/bit.252102018-03-20T10:38:37Z2014-02-252014-02-25T00:00:00ZHandlehttp://hdl.handle.net/10400.6/4650http://purl.org/coar/access_right/c_16ecrestricted accessCancerIn vitro modelsLOT3D MCTSTumor microenvironment5146305 bytesFundação para a Ciência e a TecnologiaBolsa de Doutoramento FCT: Biosynthesis and purification of minicircle DNA for application in diabetes cell-specific gene therapy [SFRH/BD/80402/2011]info:eu-repo/grantAgreement/FCT/FARH/SFRH%2FBD%2F80402%2F2011/PTSFRH/BD/80402/2011FARHBolsa de DoutoramentoCrossref Funder IDhttp://doi.org/10.13039/501100001871literaturehttp://purl.org/coar/resource_type/c_6501journal articlehttp://purl.org/coar/access_right/c_16ecapplication/pdffulltexthttps://ubibliorum.ubi.pt/bitstreams/6e29c2da-bdbb-40b1-8b29-1fecad6cdaef/downloadBiotechnology and Bioengineering11116721685 |
| spellingShingle | Optimization of liquid overlay technique to formulate heterogenic 3D co‐cultures models Costa, Elisabete C. Cancer In vitro models LOT 3D MCTS Tumor microenvironment |
| status | SINGLETON |
| subject.fl_str_mv | Cancer In vitro models LOT 3D MCTS Tumor microenvironment |
| title | Optimization of liquid overlay technique to formulate heterogenic 3D co‐cultures models |
| title_full | Optimization of liquid overlay technique to formulate heterogenic 3D co‐cultures models |
| title_fullStr | Optimization of liquid overlay technique to formulate heterogenic 3D co‐cultures models |
| title_full_unstemmed | Optimization of liquid overlay technique to formulate heterogenic 3D co‐cultures models |
| title_short | Optimization of liquid overlay technique to formulate heterogenic 3D co‐cultures models |
| title_sort | Optimization of liquid overlay technique to formulate heterogenic 3D co‐cultures models |
| topic | Cancer In vitro models LOT 3D MCTS Tumor microenvironment |
| topic_facet | Cancer In vitro models LOT 3D MCTS Tumor microenvironment |
| url | http://hdl.handle.net/10400.6/4650 |
| visible | 1 |