Publicação
Effect of amniotic membrane proteins in human cancer cell lines: an exploratory study
| Resumo: | Human amniotic membrane (hAM) has recently drawn attention as an upcoming anti-cancer therapy. Regarding the strategies which have already investigated, little is known about hAM protein extracts (hAMPE) effect on cancer. So, this work aims to study the effect of hAMPE in metabolic activity of several human cancer cell lines. hAMPE were mechanically obtained, thus avoiding the effect of detergents and other reagents commonly used in protein extraction under the cell lines studied. After quantification of proteins in hAMPE, their effect on the metabolic activity of 21 human cancer cell lines was assessed by 3-(4,5-dimethylthia-zolyl-2)2,5-diphenyltetrazolium bromide (MTT) assay. Our results indicate that there is an inhibition of metabolic activity until 25 and 50% in two and seven cell lines, respectively. Five cell lines proved to be very sensitive to hAMPE, being its metabolic activity more than 50% inhibited. Our results show that hAMPE can inhibit the metabolic activity of some human cancer cell lines. However, research about this cell line-dependent response to hAMPE becomes indispensable. |
|---|---|
| Autores principais: | Mamede, AC |
| Outros Autores: | Laranjo, Marta; Carvalho, Maria J; Abrantes, Ana M; Pires, A S; Brito, A F; Moura, P; Maia, C J; Botelho, M F |
| Assunto: | Amniotic membrane Cancer Cell Lines Metabolic activity MTT Assay |
| Ano: | 2014 |
| País: | Portugal |
| Tipo de documento: | artigo |
| Tipo de acesso: | acesso restrito |
| Instituição associada: | Universidade da Beira Interior |
| Idioma: | inglês |
| Origem: | uBibliorum |
| _version_ | 1868421018762084352 |
|---|---|
| author | Mamede, AC |
| author2 | Laranjo, Marta Carvalho, Maria J Abrantes, Ana M Pires, A S Brito, A F Moura, P Maia, C J Botelho, M F |
| author2_role | author author author author author author author author |
| author_facet | Mamede, AC Laranjo, Marta Carvalho, Maria J Abrantes, Ana M Pires, A S Brito, A F Moura, P Maia, C J Botelho, M F |
| author_role | author |
| contributor_name_str_mv | uBibliorum |
| country_str | PT |
| creators_json_txt | [{\"Person.name\":\"Mamede, AC\",\"Person.identifier.orcid\":\"0000-0002-6149-3190\"},{\"Person.name\":\"Laranjo, Marta\",\"Person.identifier.orcid\":\"0000-0002-3900-5592\"},{\"Person.name\":\"Carvalho, Maria J\",\"Person.identifier.orcid\":\"0000-0001-6819-927X\"},{\"Person.name\":\"Abrantes, Ana M\",\"Person.identifier.orcid\":\"0000-0003-4185-7871\"},{\"Person.name\":\"Pires, A S\",\"Person.identifier.orcid\":\"0000-0001-7994-4650\"},{\"Person.name\":\"Brito, A F\"},{\"Person.name\":\"Moura, P\"},{\"Person.name\":\"Maia, C J\",\"Person.identifier.orcid\":\"0000-0002-5658-5445\"},{\"Person.name\":\"Botelho, M F\",\"Person.identifier.orcid\":\"0000-0001-7202-1650\"}] |
| datacite.contributors.contributor.contributorName.fl_str_mv | uBibliorum |
| datacite.creators.creator.creatorName.fl_str_mv | Mamede, AC Laranjo, Marta Carvalho, Maria J Abrantes, Ana M Pires, A S Brito, A F Moura, P Maia, C J Botelho, M F |
| datacite.date.Accepted.fl_str_mv | 2014-04-01T00:00:00Z |
| datacite.date.available.fl_str_mv | 2019-12-02T16:45:37Z |
| datacite.date.embargoed.fl_str_mv | 2019-12-02T16:45:37Z |
| datacite.rights.fl_str_mv | http://purl.org/coar/access_right/c_16ec |
| datacite.subjects.subject.fl_str_mv | Amniotic membrane Cancer Cell Lines Metabolic activity MTT Assay |
| datacite.titles.title.fl_str_mv | Effect of amniotic membrane proteins in human cancer cell lines: an exploratory study |
| dc.contributor.none.fl_str_mv | uBibliorum |
| dc.creator.none.fl_str_mv | Mamede, AC Laranjo, Marta Carvalho, Maria J Abrantes, Ana M Pires, A S Brito, A F Moura, P Maia, C J Botelho, M F |
| dc.date.Accepted.fl_str_mv | 2014-04-01T00:00:00Z |
| dc.date.available.fl_str_mv | 2019-12-02T16:45:37Z |
| dc.date.embargoed.fl_str_mv | 2019-12-02T16:45:37Z |
| dc.format.none.fl_str_mv | application/pdf |
| dc.identifier.none.fl_str_mv | http://hdl.handle.net/10400.6/7647 |
| dc.language.none.fl_str_mv | eng |
| dc.rights.none.fl_str_mv | http://purl.org/coar/access_right/c_16ec |
| dc.subject.none.fl_str_mv | Amniotic membrane Cancer Cell Lines Metabolic activity MTT Assay |
| dc.title.fl_str_mv | Effect of amniotic membrane proteins in human cancer cell lines: an exploratory study |
| dc.type.none.fl_str_mv | http://purl.org/coar/resource_type/c_6501 |
| description | Human amniotic membrane (hAM) has recently drawn attention as an upcoming anti-cancer therapy. Regarding the strategies which have already investigated, little is known about hAM protein extracts (hAMPE) effect on cancer. So, this work aims to study the effect of hAMPE in metabolic activity of several human cancer cell lines. hAMPE were mechanically obtained, thus avoiding the effect of detergents and other reagents commonly used in protein extraction under the cell lines studied. After quantification of proteins in hAMPE, their effect on the metabolic activity of 21 human cancer cell lines was assessed by 3-(4,5-dimethylthia-zolyl-2)2,5-diphenyltetrazolium bromide (MTT) assay. Our results indicate that there is an inhibition of metabolic activity until 25 and 50% in two and seven cell lines, respectively. Five cell lines proved to be very sensitive to hAMPE, being its metabolic activity more than 50% inhibited. Our results show that hAMPE can inhibit the metabolic activity of some human cancer cell lines. However, research about this cell line-dependent response to hAMPE becomes indispensable. |
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| format | article |
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| funding.identifier_str_mv | SFRH/BD/73649/2010 SFRH/BD/44957/2008 SFRH/SINTD/60068/2009 SFRH/BD/75300/2010 SFRH/BD/61378/2009 |
| funding.name_str_mv | SFRH Bolsa de Doutoramento SFRH SFRH Bolsa de Doutoramento SFRH |
| funding_str_mv | SFRH/BD/73649/2010 info:eu-repo/grantAgreement/FCT/SFRH/SFRH%2FBD%2F73649%2F2010/PT SFRH/BD/44957/2008 info:eu-repo/grantAgreement/FCT/SFRH/SFRH%2FBD%2F44957%2F2008/PT SFRH/SINTD/60068/2009 info:eu-repo/grantAgreement/FCT/SFRH/SFRH%2FSINTD%2F60068%2F2009/PT SFRH/BD/75300/2010 info:eu-repo/grantAgreement/FCT//SFRH%2FBD%2F75300%2F2010/PT SFRH/BD/61378/2009 info:eu-repo/grantAgreement/FCT/SFRH/SFRH%2FBD%2F61378%2F2009/PT |
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| identifier.url.fl_str_mv | http://hdl.handle.net/10400.6/7647 |
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| institution | Universidade da Beira Interior |
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| language | eng |
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| person_str_mv | Mamede, AC Mamede, AC https://www.ciencia-id.pt/1811-97D9-3899 1811-97D9-3899 http://orcid.org/0000-0002-6149-3190 0000-0002-6149-3190 Laranjo, Marta Laranjo, Marta https://www.ciencia-id.pt/861A-EEAC-70E0 861A-EEAC-70E0 http://orcid.org/0000-0002-3900-5592 0000-0002-3900-5592 Carvalho, Maria J Carvalho, Maria J https://www.ciencia-id.pt/2B1C-C120-B274 2B1C-C120-B274 http://orcid.org/0000-0001-6819-927X 0000-0001-6819-927X Abrantes, Ana M Abrantes, Ana M https://www.ciencia-id.pt/7818-2E25-9410 7818-2E25-9410 http://orcid.org/0000-0003-4185-7871 0000-0003-4185-7871 Pires, A S Pires, A S https://www.ciencia-id.pt/1A1F-A52B-D09F 1A1F-A52B-D09F http://orcid.org/0000-0001-7994-4650 0000-0001-7994-4650 Brito, A F Moura, P Maia, C J Maia, C J https://www.ciencia-id.pt/5115-FCBD-A31F 5115-FCBD-A31F http://orcid.org/0000-0002-5658-5445 0000-0002-5658-5445 Botelho, M F Botelho, M F https://www.ciencia-id.pt/D716-3387-EAC1 D716-3387-EAC1 http://orcid.org/0000-0001-7202-1650 0000-0001-7202-1650 |
| publishDate | 2014 |
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| spelling | engpt_PTHuman amniotic membrane (hAM) has recently drawn attention as an upcoming anti-cancer therapy. Regarding the strategies which have already investigated, little is known about hAM protein extracts (hAMPE) effect on cancer. So, this work aims to study the effect of hAMPE in metabolic activity of several human cancer cell lines. hAMPE were mechanically obtained, thus avoiding the effect of detergents and other reagents commonly used in protein extraction under the cell lines studied. After quantification of proteins in hAMPE, their effect on the metabolic activity of 21 human cancer cell lines was assessed by 3-(4,5-dimethylthia-zolyl-2)2,5-diphenyltetrazolium bromide (MTT) assay. Our results indicate that there is an inhibition of metabolic activity until 25 and 50% in two and seven cell lines, respectively. Five cell lines proved to be very sensitive to hAMPE, being its metabolic activity more than 50% inhibited. Our results show that hAMPE can inhibit the metabolic activity of some human cancer cell lines. However, research about this cell line-dependent response to hAMPE becomes indispensable.application/pdfpt_PTEffect of amniotic membrane proteins in human cancer cell lines: an exploratory studyPersonalMamede, ACDSpacehttp://dspace.org/items/a7b98390-29cc-4e9b-9129-3083545c1ad1DSpacehttp://dspace.org/items/a7b98390-29cc-4e9b-9129-3083545c1ad1MamedeAna Catarina ManjolinhaCiência IDhttps://www.ciencia-id.pt1811-97D9-3899ORCIDhttp://orcid.org0000-0002-6149-3190Google Scholar IDhttps://scholar.google.comClNSflIAAAAJ&hlPersonalLaranjo, MartaDSpacehttp://dspace.org/items/1758cb3f-370e-417d-91cd-d4822373b6b4DSpacehttp://dspace.org/items/1758cb3f-370e-417d-91cd-d4822373b6b4LaranjoMartaCiência IDhttps://www.ciencia-id.pt861A-EEAC-70E0ORCIDhttp://orcid.org0000-0002-3900-5592Scopus Author IDhttps://www.scopus.com7801545833PersonalCarvalho, Maria JDSpacehttp://dspace.org/items/083bb301-7670-4417-9642-0143940c0a4dDSpacehttp://dspace.org/items/083bb301-7670-4417-9642-0143940c0a4dCarvalhoMaria JCiência IDhttps://www.ciencia-id.pt2B1C-C120-B274ORCIDhttp://orcid.org0000-0001-6819-927XScopus Author IDhttps://www.scopus.com55440132400Scopus Author IDhttps://www.scopus.com57198404419PersonalAbrantes, Ana MDSpacehttp://dspace.org/items/67a1671d-1c2c-4a42-ad0e-6213e203e560DSpacehttp://dspace.org/items/67a1671d-1c2c-4a42-ad0e-6213e203e560AbrantesAna MCiência IDhttps://www.ciencia-id.pt7818-2E25-9410ORCIDhttp://orcid.org0000-0003-4185-7871Researcher IDhttps://www.researcherid.comG-6913-2018Scopus Author IDhttps://www.scopus.com26029747600PersonalPires, A SDSpacehttp://dspace.org/items/361cddf1-5bf8-4758-a0bd-481853b62110DSpacehttp://dspace.org/items/361cddf1-5bf8-4758-a0bd-481853b62110Pires LourençoAna SaloméCiência IDhttps://www.ciencia-id.pt1A1F-A52B-D09FORCIDhttp://orcid.org0000-0001-7994-4650Scopus Author IDhttps://www.scopus.com56401575000Brito, A FMoura, PPersonalMaia, C JDSpacehttp://dspace.org/items/1080c532-c737-4b60-bc44-1d18e3c877d7DSpacehttp://dspace.org/items/1080c532-c737-4b60-bc44-1d18e3c877d7Maia BaptistaCláudio JorgeCiência IDhttps://www.ciencia-id.pt5115-FCBD-A31FORCIDhttp://orcid.org0000-0002-5658-5445Scopus Author IDhttps://www.scopus.com23097426600PersonalBotelho, M FDSpacehttp://dspace.org/items/a8825cf9-7020-473f-baa2-f4a5738dc962DSpacehttp://dspace.org/items/a8825cf9-7020-473f-baa2-f4a5738dc962BotelhoMaria FilomenaCiência IDhttps://www.ciencia-id.ptD716-3387-EAC1ORCIDhttp://orcid.org0000-0001-7202-1650Researcher IDhttps://www.researcherid.comG-1860-2011Scopus Author IDhttps://www.scopus.com7005751167HostingInstitutionOrganizationaluBibliorume-mailmailto:noreplyuBibliorum@ubi.ptnoreplyuBibliorum@ubi.ptDOIIsPartOf10.1007/s00232-014-9642-32019-12-02T16:45:37Z2014-042014-04-01T00:00:00ZHandlehttp://hdl.handle.net/10400.6/7647http://purl.org/coar/access_right/c_16ecrestricted accessAmniotic membraneCancer Cell LinesMetabolic activityMTT Assay176357 bytesFundação para a Ciência e a TecnologiaBolsa de Doutoramento FCT - Estudo da membrana amniótica humana e o seu efeito em linhagens celulares de cancro, particularmente em carcinoma hepatocelular (cancro do fígado) [SFRH/BD/73649/2010]info:eu-repo/grantAgreement/FCT/SFRH/SFRH%2FBD%2F73649%2F2010/PTSFRH/BD/73649/2010SFRHBolsa de DoutoramentoCrossref Funder IDhttp://doi.org/10.13039/501100001871Fundação para a Ciência e a TecnologiaBolsa de doutoramento FCT: Marcação com 99mTc de Sensibilizadores para Imagiologia do Tratamento Fotodinâmico [SFRH/BD/44957/2008]info:eu-repo/grantAgreement/FCT/SFRH/SFRH%2FBD%2F44957%2F2008/PTSFRH/BD/44957/2008SFRHCrossref Funder IDhttp://doi.org/10.13039/501100001871Fundação para a Ciência e a TecnologiaBolsa de investigação FCT: Células tumorais do cancro do endométrio: caracterização, resposta à terapêutica e padrão de metastização in vivo [SFRH/SINTD/60068/2009]info:eu-repo/grantAgreement/FCT/SFRH/SFRH%2FSINTD%2F60068%2F2009/PTSFRH/SINTD/60068/2009SFRHCrossref Funder IDhttp://doi.org/10.13039/501100001871Fundação para a Ciência e a TecnologiaBolsa de Doutoramento FCT - Vitamina C e Cancro: Estudo Experimental [SFRH/BD/75300/2010]info:eu-repo/grantAgreement/FCT//SFRH%2FBD%2F75300%2F2010/PTSFRH/BD/75300/2010Bolsa de DoutoramentoCrossref Funder IDhttp://doi.org/10.13039/501100001871Fundação para a Ciência e a TecnologiaBolsa de doutoramento FCT [SFRH/BD/61378/2009]info:eu-repo/grantAgreement/FCT/SFRH/SFRH%2FBD%2F61378%2F2009/PTSFRH/BD/61378/2009SFRHCrossref Funder IDhttp://doi.org/10.13039/501100001871literaturehttp://purl.org/coar/resource_type/c_6501journal articlehttp://purl.org/coar/access_right/c_16ecapplication/pdffulltexthttps://ubibliorum.ubi.pt/bitstreams/3bac8cdd-c749-4a24-a7c5-740811bfc955/downloadJournal of Membrane Biology2474357360 |
| spellingShingle | Effect of amniotic membrane proteins in human cancer cell lines: an exploratory study Mamede, AC Amniotic membrane Cancer Cell Lines Metabolic activity MTT Assay |
| status | SINGLETON |
| subject.fl_str_mv | Amniotic membrane Cancer Cell Lines Metabolic activity MTT Assay |
| title | Effect of amniotic membrane proteins in human cancer cell lines: an exploratory study |
| title_full | Effect of amniotic membrane proteins in human cancer cell lines: an exploratory study |
| title_fullStr | Effect of amniotic membrane proteins in human cancer cell lines: an exploratory study |
| title_full_unstemmed | Effect of amniotic membrane proteins in human cancer cell lines: an exploratory study |
| title_short | Effect of amniotic membrane proteins in human cancer cell lines: an exploratory study |
| title_sort | Effect of amniotic membrane proteins in human cancer cell lines: an exploratory study |
| topic | Amniotic membrane Cancer Cell Lines Metabolic activity MTT Assay |
| topic_facet | Amniotic membrane Cancer Cell Lines Metabolic activity MTT Assay |
| url | http://hdl.handle.net/10400.6/7647 |
| visible | 1 |