Publicação
Highly selective capture of minicircle DNA biopharmaceuticals by a novel zinc-histidine peptide conjugate
| Resumo: | The use of minicircle DNA (mcDNA) biomolecules as a pharmaceutical product holds remarkable potential due to their improved therapeutic efficacy in comparison with standard non-viral gene expression vectors. However, mcDNA translation into clinical application is still highly restricted due to the lack of robust technologies for minicircles detection and purification. In this study, the potential of a zinc-binding histidine-based peptide to function as a novel ligand for mcDNA recovery was investigated by using high-throughput surface plasmon resonance (SPR) analysis. The histidine-based peptide successfully bound zinc cationic ions and had affinity towards mcDNA biomolecules as confirmed by the dynamic binding responses obtained in SPR experiments. Notably, the obtained results indicate that not only zinc-peptide ligands are able to bind mcDNA with very high affinity (KD = 4.21 × 10−10 M), but also that this interaction is mostly dependent on buffer type. In general, the findings indicated that Zn2+ bound peptide has high affinity to mcDNA in low ionic strength buffers, whereas with high salt buffers no binding is detected. Overall, the novel zinc-binding peptide has shown to have suitable properties for mcDNA binding and recovery under experimental conditions that assure genetic material stability. More importantly, the straightforward approach of employing simple biomimetic ligands for mcDNA capture will contribute for development of new technologies to purify DNA biopharmaceuticals. |
|---|---|
| Autores principais: | Gaspar, Vítor Manuel Abreu |
| Outros Autores: | Cruz, Carla Patrícia Alves Freire Madeira; Queiroz, João; Pichon, Chantal; Correia, Ilídio; Sousa, Fani |
| Assunto: | Minicircle DNA Affinity interactions Biopharmaceuticals Biomimetic peptide |
| Ano: | 2016 |
| País: | Portugal |
| Tipo de documento: | artigo |
| Tipo de acesso: | acesso restrito |
| Instituição associada: | Universidade da Beira Interior |
| Idioma: | inglês |
| Origem: | uBibliorum |
| _version_ | 1868420920776851456 |
|---|---|
| author | Gaspar, Vítor Manuel Abreu |
| author2 | Cruz, Carla Patrícia Alves Freire Madeira Queiroz, João Pichon, Chantal Correia, Ilídio Sousa, Fani |
| author2_role | author author author author author |
| author_facet | Gaspar, Vítor Manuel Abreu Cruz, Carla Patrícia Alves Freire Madeira Queiroz, João Pichon, Chantal Correia, Ilídio Sousa, Fani |
| author_role | author |
| contributor_name_str_mv | uBibliorum |
| country_str | PT |
| creators_json_txt | [{\"Person.name\":\"Gaspar, Vítor Manuel Abreu\",\"Person.identifier.orcid\":\"0000-0002-0372-2493\"},{\"Person.name\":\"Cruz, Carla Patrícia Alves Freire Madeira\"},{\"Person.name\":\"Queiroz, João\",\"Person.identifier.orcid\":\"0000-0002-3096-8325\"},{\"Person.name\":\"Pichon, Chantal\"},{\"Person.name\":\"Correia, Ilídio\",\"Person.identifier.orcid\":\"0000-0003-1613-9675\"},{\"Person.name\":\"Sousa, Fani\",\"Person.identifier.orcid\":\"0000-0001-9996-2194\"}] |
| datacite.contributors.contributor.contributorName.fl_str_mv | uBibliorum |
| datacite.creators.creator.creatorName.fl_str_mv | Gaspar, Vítor Manuel Abreu Cruz, Carla Patrícia Alves Freire Madeira Queiroz, João Pichon, Chantal Correia, Ilídio Sousa, Fani |
| datacite.date.Accepted.fl_str_mv | 2016-10-31T00:00:00Z |
| datacite.date.available.fl_str_mv | 2018-03-22T08:45:52Z |
| datacite.date.embargoed.fl_str_mv | 2018-03-22T08:45:52Z |
| datacite.rights.fl_str_mv | http://purl.org/coar/access_right/c_16ec |
| datacite.subjects.subject.fl_str_mv | Minicircle DNA Affinity interactions Biopharmaceuticals Biomimetic peptide |
| datacite.titles.title.fl_str_mv | Highly selective capture of minicircle DNA biopharmaceuticals by a novel zinc-histidine peptide conjugate |
| dc.contributor.none.fl_str_mv | uBibliorum |
| dc.creator.none.fl_str_mv | Gaspar, Vítor Manuel Abreu Cruz, Carla Patrícia Alves Freire Madeira Queiroz, João Pichon, Chantal Correia, Ilídio Sousa, Fani |
| dc.date.Accepted.fl_str_mv | 2016-10-31T00:00:00Z |
| dc.date.available.fl_str_mv | 2018-03-22T08:45:52Z |
| dc.date.embargoed.fl_str_mv | 2018-03-22T08:45:52Z |
| dc.format.none.fl_str_mv | application/pdf |
| dc.identifier.none.fl_str_mv | http://hdl.handle.net/10400.6/4693 |
| dc.language.none.fl_str_mv | eng |
| dc.publisher.none.fl_str_mv | Elsevier |
| dc.rights.none.fl_str_mv | http://purl.org/coar/access_right/c_16ec |
| dc.subject.none.fl_str_mv | Minicircle DNA Affinity interactions Biopharmaceuticals Biomimetic peptide |
| dc.title.fl_str_mv | Highly selective capture of minicircle DNA biopharmaceuticals by a novel zinc-histidine peptide conjugate |
| dc.type.none.fl_str_mv | http://purl.org/coar/resource_type/c_6501 |
| description | The use of minicircle DNA (mcDNA) biomolecules as a pharmaceutical product holds remarkable potential due to their improved therapeutic efficacy in comparison with standard non-viral gene expression vectors. However, mcDNA translation into clinical application is still highly restricted due to the lack of robust technologies for minicircles detection and purification. In this study, the potential of a zinc-binding histidine-based peptide to function as a novel ligand for mcDNA recovery was investigated by using high-throughput surface plasmon resonance (SPR) analysis. The histidine-based peptide successfully bound zinc cationic ions and had affinity towards mcDNA biomolecules as confirmed by the dynamic binding responses obtained in SPR experiments. Notably, the obtained results indicate that not only zinc-peptide ligands are able to bind mcDNA with very high affinity (KD = 4.21 × 10−10 M), but also that this interaction is mostly dependent on buffer type. In general, the findings indicated that Zn2+ bound peptide has high affinity to mcDNA in low ionic strength buffers, whereas with high salt buffers no binding is detected. Overall, the novel zinc-binding peptide has shown to have suitable properties for mcDNA binding and recovery under experimental conditions that assure genetic material stability. More importantly, the straightforward approach of employing simple biomimetic ligands for mcDNA capture will contribute for development of new technologies to purify DNA biopharmaceuticals. |
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| format | article |
| fulltext.url.fl_str_mv | https://ubibliorum.ubi.pt/bitstreams/83c2238f-5a04-4f3a-930e-d0c3fe06b5da/download |
| funding.funder.alternateName_str_mv | FCT FCT FCT |
| funding.funder.identifier_str_mv | http://doi.org/10.13039/501100001871 http://doi.org/10.13039/501100001871 http://doi.org/10.13039/501100001871 |
| funding.funder.name_str_mv | Fundação para a Ciência e a Tecnologia Fundação para a Ciência e a Tecnologia Fundação para a Ciência e a Tecnologia |
| funding.identifier_str_mv | PEst-C/SAU/UI0709/2011 EXPL/QEQ-MED/1068/2013 SFRH/BPD/100015/2014 |
| funding.name_str_mv | 6820 - DCRRNI ID 5876-PPCDTI Bolsa de Pós-Doutoramento |
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| identifier.url.fl_str_mv | http://hdl.handle.net/10400.6/4693 |
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| institution | Universidade da Beira Interior |
| instname_str | Universidade da Beira Interior |
| language | eng |
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| person_str_mv | Gaspar, Vítor Manuel Abreu Gaspar, Vítor Manuel Abreu https://www.ciencia-id.pt/6F16-3640-73E3 6F16-3640-73E3 http://orcid.org/0000-0002-0372-2493 0000-0002-0372-2493 Cruz, Carla Patrícia Alves Freire Madeira Queiroz, João Queiroz, João https://www.ciencia-id.pt/931E-B66D-E341 931E-B66D-E341 http://orcid.org/0000-0002-3096-8325 0000-0002-3096-8325 Pichon, Chantal Correia, Ilídio Correia, Ilídio https://www.ciencia-id.pt/F610-7373-DC81 F610-7373-DC81 http://orcid.org/0000-0003-1613-9675 0000-0003-1613-9675 Sousa, Fani Sousa, Fani https://www.ciencia-id.pt/991D-2E13-A840 991D-2E13-A840 http://orcid.org/0000-0001-9996-2194 0000-0001-9996-2194 |
| publishDate | 2016 |
| publisher.none.fl_str_mv | Elsevier |
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| spelling | engElsevierpt_PTThe use of minicircle DNA (mcDNA) biomolecules as a pharmaceutical product holds remarkable potential due to their improved therapeutic efficacy in comparison with standard non-viral gene expression vectors. However, mcDNA translation into clinical application is still highly restricted due to the lack of robust technologies for minicircles detection and purification. In this study, the potential of a zinc-binding histidine-based peptide to function as a novel ligand for mcDNA recovery was investigated by using high-throughput surface plasmon resonance (SPR) analysis. The histidine-based peptide successfully bound zinc cationic ions and had affinity towards mcDNA biomolecules as confirmed by the dynamic binding responses obtained in SPR experiments. Notably, the obtained results indicate that not only zinc-peptide ligands are able to bind mcDNA with very high affinity (KD = 4.21 × 10−10 M), but also that this interaction is mostly dependent on buffer type. In general, the findings indicated that Zn2+ bound peptide has high affinity to mcDNA in low ionic strength buffers, whereas with high salt buffers no binding is detected. Overall, the novel zinc-binding peptide has shown to have suitable properties for mcDNA binding and recovery under experimental conditions that assure genetic material stability. More importantly, the straightforward approach of employing simple biomimetic ligands for mcDNA capture will contribute for development of new technologies to purify DNA biopharmaceuticals.application/pdfpt_PTHighly selective capture of minicircle DNA biopharmaceuticals by a novel zinc-histidine peptide conjugatePersonalGaspar, Vítor Manuel AbreuDSpacehttp://dspace.org/items/3eeac15e-ebf4-4dc2-aeb8-698087d6d2fcDSpacehttp://dspace.org/items/3eeac15e-ebf4-4dc2-aeb8-698087d6d2fcGasparVítorCiência IDhttps://www.ciencia-id.pt6F16-3640-73E3ORCIDhttp://orcid.org0000-0002-0372-2493Researcher IDhttps://www.researcherid.comB-1602-2017Scopus Author IDhttps://www.scopus.com36968590900Cruz, Carla Patrícia Alves Freire MadeiraPersonalQueiroz, JoãoDSpacehttp://dspace.org/items/c798c033-c160-4f99-a8ad-04416923943fDSpacehttp://dspace.org/items/c798c033-c160-4f99-a8ad-04416923943fQueirozJoãoCiência IDhttps://www.ciencia-id.pt931E-B66D-E341ORCIDhttp://orcid.org0000-0002-3096-8325Researcher IDhttps://www.researcherid.comL-3104-2014Scopus Author IDhttps://www.scopus.com7003705645Pichon, ChantalPersonalCorreia, IlídioDSpacehttp://dspace.org/items/f2c3e779-34a2-4309-950a-80687664c6adDSpacehttp://dspace.org/items/f2c3e779-34a2-4309-950a-80687664c6adCorreiaIlídio Joaquim SobreiraCiência IDhttps://www.ciencia-id.ptF610-7373-DC81ORCIDhttp://orcid.org0000-0003-1613-9675Scopus Author IDhttps://www.scopus.com7003557499PersonalSousa, FaniDSpacehttp://dspace.org/items/2935de97-fc90-4d38-9505-4caa364f8a10DSpacehttp://dspace.org/items/2935de97-fc90-4d38-9505-4caa364f8a10SousaFaniCiência IDhttps://www.ciencia-id.pt991D-2E13-A840ORCIDhttp://orcid.org0000-0001-9996-2194Researcher IDhttps://www.researcherid.comA-2014-2017Scopus Author IDhttps://www.scopus.com7005110268HostingInstitutionOrganizationaluBibliorume-mailmailto:noreplyuBibliorum@ubi.ptnoreplyuBibliorum@ubi.ptDOIIsPartOf10.1016/j.seppur.2016.10.0542018-03-22T08:45:52Z2016-10-312016-10-31T00:00:00ZHandlehttp://hdl.handle.net/10400.6/4693http://purl.org/coar/access_right/c_16ecrestricted accessMinicircle DNAAffinity interactionsBiopharmaceuticalsBiomimetic peptide1582020 bytesFundação para a Ciência e a TecnologiaStrategic Project - UI 709 - 2011-2012 [PEst-C/SAU/UI0709/2011]info:eu-repo/grantAgreement/FCT/6820 - DCRRNI ID/PEst-C%2FSAU%2FUI0709%2F2011/PTPEst-C/SAU/UI0709/20116820 - DCRRNI IDCrossref Funder IDhttp://doi.org/10.13039/501100001871Fundação para a Ciência e a TecnologiaG-quadruplex binders for cancer therapy [EXPL/QEQ-MED/1068/2013]info:eu-repo/grantAgreement/FCT/5876-PPCDTI/EXPL%2FQEQ-MED%2F1068%2F2013/PTEXPL/QEQ-MED/1068/20135876-PPCDTICrossref Funder IDhttp://doi.org/10.13039/501100001871Fundação para a Ciência e a TecnologiaBolsa de Pós-Doutoramento FCT: Purification, detection and stabilization of G-quadruplex for cancer therapy [SFRH/BPD/100015/2014]info:eu-repo/grantAgreement/FCT//SFRH%2FBPD%2F100015%2F2014/PTSFRH/BPD/100015/2014Bolsa de Pós-DoutoramentoCrossref Funder IDhttp://doi.org/10.13039/501100001871literaturehttp://purl.org/coar/resource_type/c_6501journal articlehttp://purl.org/coar/access_right/c_16ecapplication/pdffulltexthttps://ubibliorum.ubi.pt/bitstreams/83c2238f-5a04-4f3a-930e-d0c3fe06b5da/downloadSeparation and Purification Technology174417424 |
| spellingShingle | Highly selective capture of minicircle DNA biopharmaceuticals by a novel zinc-histidine peptide conjugate Gaspar, Vítor Manuel Abreu Minicircle DNA Affinity interactions Biopharmaceuticals Biomimetic peptide |
| status | SINGLETON |
| subject.fl_str_mv | Minicircle DNA Affinity interactions Biopharmaceuticals Biomimetic peptide |
| title | Highly selective capture of minicircle DNA biopharmaceuticals by a novel zinc-histidine peptide conjugate |
| title_full | Highly selective capture of minicircle DNA biopharmaceuticals by a novel zinc-histidine peptide conjugate |
| title_fullStr | Highly selective capture of minicircle DNA biopharmaceuticals by a novel zinc-histidine peptide conjugate |
| title_full_unstemmed | Highly selective capture of minicircle DNA biopharmaceuticals by a novel zinc-histidine peptide conjugate |
| title_short | Highly selective capture of minicircle DNA biopharmaceuticals by a novel zinc-histidine peptide conjugate |
| title_sort | Highly selective capture of minicircle DNA biopharmaceuticals by a novel zinc-histidine peptide conjugate |
| topic | Minicircle DNA Affinity interactions Biopharmaceuticals Biomimetic peptide |
| topic_facet | Minicircle DNA Affinity interactions Biopharmaceuticals Biomimetic peptide |
| url | http://hdl.handle.net/10400.6/4693 |
| visible | 1 |