Publication

Implications of epigallocatechin-3-gallate in cultured human Sertoli cells glycolytic and oxidative profile

View document

Bibliographic Details
Summary:Sertoli cells are crucial for the success of spermatogenesis, which is the biological process that ensures male fertility. These cells present high metabolic rates, being often subjected to high oxidative stress levels that, if uncontrolled, may compromise male fertility. Since the most abundant tea catechin, epigallocatechin-3-gallate (EGCG), has demonstrated a potent preventive activity against oxidative stress, we have evaluated its effect at concentrations of 5 and 50μM, on the metabolism, mitochondrial functionality and oxidative profile of human Sertoli cells (hSCs). While, the highest concentration of EGCG (50μM) increased glucose and pyruvate consumption, it decreased the conversion of pyruvate to alanine to sustain a regular lactate production. However, despite maintaining Krebs cycle functionality, EGCG (50μM) decreased mitochondrial membrane potential of hSCs, which could compromise the normal rates of ATP production. Interestingly, oxidative damages to proteins and lipids decreased in this experimental group, which may be valuable for the nutritional support of spermatogenesis.
Main Authors:Dias, Tânia R.
Other Authors:Alves, Marco G; Silva, Joaquina; Barros, Alberto; Sousa, Mário; Casal, Susana; Silva, Branca M.; Oliveira, Pedro F.
Subject:Alanine Catechin Cell Proliferation Cells Cultured Glucose Glucose Transporter Type 2 Glycolysis Humans L-Lactate Dehydrogenase Male Membrane Potential Mitochondrial Monocarboxylic Acid Transporters Muscle Proteins Oxidative Stress Pyruvic Acid Sertoli Cells
Year:2017
Country:Portugal
Document type:article
Access type:open access
Associated institution:Universidade da Beira Interior
Language:English
Origin:uBibliorum

Similar Items

Funded activities

Loading funded projects...

View more

Similar Items