Publication
Effects of salinity challenge on the endocrine control of osmoregulation and calcium homeostasis in the sea bream
| Summary: | The gilthead sea bream (Sparus auratu) is a marine species often found in coastal lagoons, experimenting episodic exposures to both brackish and hypersaline environments. However, little is known about the underlying endocrine mechanisms controlling osmoregulation in this and in most marine species. This study aimed at characterising some of the endocrine basis of sea bream osmoregulation, with emphasis on calcium homeostasis. Juvenile fish were exposed to different salinities, either by direct transfer or continuous adaptation over a short period of time. Salinities ranged from 0 to 55 p.p.t. and sampling was carried out 4, 24, 96 and 192 h after transfer. Six fish per group and per time point were sacrificed and plasma and tissue samples were collected. Osmolarity, osmolites and cortisol were measured in plasma. Prolactin, growth hormone, stanniocalcin, and calcitonin mRNA expressions were determined by PCR and northern blot. Mortality occurred after 4 hours in FW. Sea bream fry (2 month old, 20-60 may) were exposed to hypersaline and dilute seawater loaded with Ca and calcium fluxes were determined. Exposure of fry to lowered external salinity (50 and 25% SW) resulted in no mortality within 24 h and significantly decreased whole body calcium influx. Results will be discussed in relation to gene expression. |
|---|---|
| Main Authors: | Guerreiro, P. M. |
| Other Authors: | Fuentes, J.; Flik, G.; Canario, Adelino V. M.; Power, Deborah |
| Year: | 1999 |
| Country: | Portugal |
| Document type: | article |
| Access type: | open access |
| Associated institution: | Universidade do Algarve |
| Language: | English |
| Origin: | Sapientia - Universidade do Algarve |
| _version_ | 1877785105810849792 |
|---|---|
| author | Guerreiro, P. M. |
| author2 | Fuentes, J. Flik, G. Canario, Adelino V. M. Power, Deborah |
| author2_role | author author author author |
| author_facet | Guerreiro, P. M. Fuentes, J. Flik, G. Canario, Adelino V. M. Power, Deborah |
| author_role | author |
| contributor_name_str_mv | Sapientia |
| country_str | PT |
| creators_json_txt | [{\"Person.name\":\"Guerreiro, P. M.\",\"Person.identifier.cienciaID\":\"5C13-965D-3148\",\"Person.identifier.orcid\":\"0000-0001-5371-7919\"},{\"Person.name\":\"Fuentes, J.\",\"Person.identifier.cienciaID\":\"421B-E196-2C33\",\"Person.identifier.orcid\":\"0000-0003-0430-8734\",\"Person.identifier.scopusAuthorID\":\"7201832526\",\"Person.identifier.scopusAuthorID\":\"57197279468\"},{\"Person.name\":\"Flik, G.\"},{\"Person.name\":\"Canario, Adelino V. M.\",\"Person.identifier.cienciaID\":\"1F1E-D3B3-F804\",\"Person.identifier.orcid\":\"0000-0002-6244-6468\",\"Person.identifier.researcherID\":\"C-7942-2009\",\"Person.identifier.scopusAuthorID\":\"56568523700\"},{\"Person.name\":\"Power, Deborah\",\"Person.identifier.cienciaID\":\"891A-8A44-3CAE\",\"Person.identifier.orcid\":\"0000-0003-1366-0246\",\"Person.identifier.scopusAuthorID\":\"7101806760\"}] |
| datacite.contributors.contributor.contributorName.fl_str_mv | Sapientia |
| datacite.creators.creator.creatorName.fl_str_mv | Guerreiro, P. M. Fuentes, J. Flik, G. Canario, Adelino V. M. Power, Deborah |
| datacite.date.Accepted.fl_str_mv | 1999-01-01T00:00:00Z |
| datacite.date.available.fl_str_mv | 2014-10-24T13:59:52Z |
| datacite.date.embargoed.fl_str_mv | 2014-10-24T13:59:52Z |
| datacite.rights.fl_str_mv | http://purl.org/coar/access_right/c_abf2 |
| datacite.titles.title.fl_str_mv | Effects of salinity challenge on the endocrine control of osmoregulation and calcium homeostasis in the sea bream |
| dc.contributor.none.fl_str_mv | Sapientia |
| dc.creator.none.fl_str_mv | Guerreiro, P. M. Fuentes, J. Flik, G. Canario, Adelino V. M. Power, Deborah |
| dc.date.Accepted.fl_str_mv | 1999-01-01T00:00:00Z |
| dc.date.available.fl_str_mv | 2014-10-24T13:59:52Z |
| dc.date.embargoed.fl_str_mv | 2014-10-24T13:59:52Z |
| dc.format.none.fl_str_mv | application/pdf |
| dc.identifier.none.fl_str_mv | http://hdl.handle.net/10400.1/5497 |
| dc.language.none.fl_str_mv | eng |
| dc.publisher.none.fl_str_mv | Elsevier |
| dc.rights.none.fl_str_mv | http://purl.org/coar/access_right/c_abf2 |
| dc.title.fl_str_mv | Effects of salinity challenge on the endocrine control of osmoregulation and calcium homeostasis in the sea bream |
| dc.type.none.fl_str_mv | http://purl.org/coar/resource_type/c_6501 |
| description | The gilthead sea bream (Sparus auratu) is a marine species often found in coastal lagoons, experimenting episodic exposures to both brackish and hypersaline environments. However, little is known about the underlying endocrine mechanisms controlling osmoregulation in this and in most marine species. This study aimed at characterising some of the endocrine basis of sea bream osmoregulation, with emphasis on calcium homeostasis. Juvenile fish were exposed to different salinities, either by direct transfer or continuous adaptation over a short period of time. Salinities ranged from 0 to 55 p.p.t. and sampling was carried out 4, 24, 96 and 192 h after transfer. Six fish per group and per time point were sacrificed and plasma and tissue samples were collected. Osmolarity, osmolites and cortisol were measured in plasma. Prolactin, growth hormone, stanniocalcin, and calcitonin mRNA expressions were determined by PCR and northern blot. Mortality occurred after 4 hours in FW. Sea bream fry (2 month old, 20-60 may) were exposed to hypersaline and dilute seawater loaded with Ca and calcium fluxes were determined. Exposure of fry to lowered external salinity (50 and 25% SW) resulted in no mortality within 24 h and significantly decreased whole body calcium influx. Results will be discussed in relation to gene expression. |
| dirty | 0 |
| eu_rights_str_mv | openAccess |
| format | article |
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| identifier.url.fl_str_mv | http://hdl.handle.net/10400.1/5497 |
| inst_facet_str | urn:organizationAcronym:ualg{{{_:::_}}}Universidade do Algarve |
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| language | eng |
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| network_name_str | Sapientia - Universidade do Algarve |
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| organization_str_mv | urn:organizationAcronym:ualg |
| person_str_mv | Guerreiro, P. M. Guerreiro, P. M. https://www.ciencia-id.pt/5C13-965D-3148 5C13-965D-3148 http://orcid.org/0000-0001-5371-7919 0000-0001-5371-7919 Fuentes, J. Fuentes, J. https://www.ciencia-id.pt/421B-E196-2C33 421B-E196-2C33 http://orcid.org/0000-0003-0430-8734 0000-0003-0430-8734 Flik, G. Canario, Adelino V. M. Canario, Adelino V. M. https://www.ciencia-id.pt/1F1E-D3B3-F804 1F1E-D3B3-F804 http://orcid.org/0000-0002-6244-6468 0000-0002-6244-6468 Power, Deborah Power, Deborah https://www.ciencia-id.pt/891A-8A44-3CAE 891A-8A44-3CAE http://orcid.org/0000-0003-1366-0246 0000-0003-1366-0246 |
| publishDate | 1999 |
| publisher.none.fl_str_mv | Elsevier |
| repo_facet_str | urn:repositoryAcronym:sapientia{{{_:::_}}}Sapientia - Universidade do Algarve |
| reponame_str | Sapientia - Universidade do Algarve |
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| spelling | engElsevierporThe gilthead sea bream (Sparus auratu) is a marine species often found in coastal lagoons, experimenting episodic exposures to both brackish and hypersaline environments. However, little is known about the underlying endocrine mechanisms controlling osmoregulation in this and in most marine species. This study aimed at characterising some of the endocrine basis of sea bream osmoregulation, with emphasis on calcium homeostasis. Juvenile fish were exposed to different salinities, either by direct transfer or continuous adaptation over a short period of time. Salinities ranged from 0 to 55 p.p.t. and sampling was carried out 4, 24, 96 and 192 h after transfer. Six fish per group and per time point were sacrificed and plasma and tissue samples were collected. Osmolarity, osmolites and cortisol were measured in plasma. Prolactin, growth hormone, stanniocalcin, and calcitonin mRNA expressions were determined by PCR and northern blot. Mortality occurred after 4 hours in FW. Sea bream fry (2 month old, 20-60 may) were exposed to hypersaline and dilute seawater loaded with Ca and calcium fluxes were determined. Exposure of fry to lowered external salinity (50 and 25% SW) resulted in no mortality within 24 h and significantly decreased whole body calcium influx. Results will be discussed in relation to gene expression.application/pdfporEffects of salinity challenge on the endocrine control of osmoregulation and calcium homeostasis in the sea breamPersonalGuerreiro, P. M.DSpacehttp://dspace.org/items/d0a8a148-98e7-4899-8fb0-0b8afa91e4a4DSpacehttp://dspace.org/items/d0a8a148-98e7-4899-8fb0-0b8afa91e4a4GuerreiroPedro MiguelCiência IDhttps://www.ciencia-id.pt5C13-965D-3148ORCIDhttp://orcid.org0000-0001-5371-7919PersonalFuentes, J.DSpacehttp://dspace.org/items/d354b937-b18c-4ca5-a843-04a04269d60fDSpacehttp://dspace.org/items/d354b937-b18c-4ca5-a843-04a04269d60fFuentesJuanCiência IDhttps://www.ciencia-id.pt421B-E196-2C33ORCIDhttp://orcid.org0000-0003-0430-8734Scopus Author IDhttps://www.scopus.com7201832526Scopus Author IDhttps://www.scopus.com57197279468Flik, G.PersonalCanario, Adelino V. M.DSpacehttp://dspace.org/items/5f6e51ee-9113-469e-8b9e-f30f2d452521DSpacehttp://dspace.org/items/5f6e51ee-9113-469e-8b9e-f30f2d452521CanarioAdelinoCiência IDhttps://www.ciencia-id.pt1F1E-D3B3-F804ORCIDhttp://orcid.org0000-0002-6244-6468Researcher IDhttps://www.researcherid.comC-7942-2009Scopus Author IDhttps://www.scopus.com56568523700PersonalPower, DeborahDSpacehttp://dspace.org/items/c68f5ffb-63f6-4c70-8957-29e464fb59c0DSpacehttp://dspace.org/items/c68f5ffb-63f6-4c70-8957-29e464fb59c0PowerDeborah MaryCiência IDhttps://www.ciencia-id.pt891A-8A44-3CAEORCIDhttp://orcid.org0000-0003-1366-0246Scopus Author IDhttps://www.scopus.com7101806760HostingInstitutionOrganizationalSapientiae-mailmailto:repositorio@ualg.ptrepositorio@ualg.ptISSNIsPartOf1095-6433URNIsPartOfAUT: PMG01122; DPO00386; ACA00258;DOIIsPartOfhttp://dx.doi.org/10.1016/S1095-6433(99)90314-82014-10-24T13:59:52Z19991999-01-01T00:00:00ZHandlehttp://hdl.handle.net/10400.1/5497http://purl.org/coar/access_right/c_abf2open access141878 bytesliteraturehttp://purl.org/coar/resource_type/c_6501journal articlehttp://purl.org/coar/access_right/c_abf2application/pdffulltexthttps://sapientia.ualg.pt/bitstreams/f58e0943-bf27-4a0b-a118-8accfe1dc71b/downloadComparative Biochemistry and Physiology A-molecular & Integrative Physiology124Supl.S80S80 |
| spellingShingle | Effects of salinity challenge on the endocrine control of osmoregulation and calcium homeostasis in the sea bream Guerreiro, P. M. |
| status | SINGLETON |
| title | Effects of salinity challenge on the endocrine control of osmoregulation and calcium homeostasis in the sea bream |
| title_full | Effects of salinity challenge on the endocrine control of osmoregulation and calcium homeostasis in the sea bream |
| title_fullStr | Effects of salinity challenge on the endocrine control of osmoregulation and calcium homeostasis in the sea bream |
| title_full_unstemmed | Effects of salinity challenge on the endocrine control of osmoregulation and calcium homeostasis in the sea bream |
| title_short | Effects of salinity challenge on the endocrine control of osmoregulation and calcium homeostasis in the sea bream |
| title_sort | Effects of salinity challenge on the endocrine control of osmoregulation and calcium homeostasis in the sea bream |
| url | http://hdl.handle.net/10400.1/5497 |
| visible | 1 |
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