Publicação
Influence of natural deep eutectic systems in water thermal behavior and their applications in cryopreservation
| Resumo: | Natural deep eutectic systems (NADES), which have applications as solvents for both engineering and life sciences, are mainly composed of sugars, aminoacids or organic acids. In this work NADES composed by glucose, urea and proline (G:U:P in a molar ratio of 1:1:1) and proline and glucose (P:G 5:3) were prepared and added in different mass fractions to water. By differential scanning calorimetry it was verified as the crystallization tendency of water is modified even for low fraction of NADES added. This is also observed by polarized optical microscopy which allowed following the formation of crystals with different crystalline morphologies as bulk water. Calorimetric data also shown as the crystallization temperature decreases for all P:G mixtures and this shift is more accentuated for weight fraction of NADES higher than 0.5. Crystallization is totally suppressed for NADES fraction higher than 0.7. NADES/water mixtures cytotoxicity was evaluated in vitro, revealing that they are less toxic as compared with the commonly used cryoprotective additives as dimethyl sulfoxide (DMSO). Additionally, cell viability tests with cell lines cryopreserved using DMSO and both the prepared NADES showed comparable viability. This work combines thermophysical data on NADES and evaluates it's in vitro performance, providing cues for their use in cryopreservation applications. |
|---|---|
| Autores principais: | Craveiro, Rita |
| Outros Autores: | Castro, Vânia I. B.; Viciosa, Maria Teresa; Dionísio, Madalena; Reis, R. L.; Duarte, Ana Rita C.; Paiva, Alexandre |
| Assunto: | Cytotoxicity Glucose NADES Proline Thermal properties Urea Water mixtures Electronic, Optical and Magnetic Materials Atomic and Molecular Physics, and Optics Condensed Matter Physics Spectroscopy Physical and Theoretical Chemistry Materials Chemistry |
| Ano: | 2021 |
| País: | Portugal |
| Tipo de documento: | artigo |
| Tipo de acesso: | acesso aberto |
| Instituição associada: | Universidade Nova de Lisboa |
| Idioma: | inglês |
| Origem: | Repositório Institucional da UNL |
| _version_ | 1879446606668890112 |
|---|---|
| author | Craveiro, Rita |
| author2 | Castro, Vânia I. B. Viciosa, Maria Teresa Dionísio, Madalena Reis, R. L. Duarte, Ana Rita C. Paiva, Alexandre |
| author2_role | author author author author author author |
| author_facet | Craveiro, Rita Castro, Vânia I. B. Viciosa, Maria Teresa Dionísio, Madalena Reis, R. L. Duarte, Ana Rita C. Paiva, Alexandre |
| author_role | author |
| contributor_name_str_mv | LAQV@REQUIMTE DQ - Departamento de Química Elsevier RUN |
| country_str | PT |
| creators_json_txt | [{\"Person.name\":\"Craveiro, Rita\"},{\"Person.name\":\"Castro, Vânia I. B.\"},{\"Person.name\":\"Viciosa, Maria Teresa\"},{\"Person.name\":\"Dionísio, Madalena\"},{\"Person.name\":\"Reis, R. L.\"},{\"Person.name\":\"Duarte, Ana Rita C.\"},{\"Person.name\":\"Paiva, Alexandre\"}] |
| datacite.contributors.contributor.contributorName.fl_str_mv | LAQV@REQUIMTE DQ - Departamento de Química Elsevier RUN |
| datacite.creators.creator.creatorName.fl_str_mv | Craveiro, Rita Castro, Vânia I. B. Viciosa, Maria Teresa Dionísio, Madalena Reis, R. L. Duarte, Ana Rita C. Paiva, Alexandre |
| datacite.date.Accepted.fl_str_mv | 2021-05-01T00:00:00Z |
| datacite.date.available.fl_str_mv | 2021-10-09T04:55:36Z |
| datacite.date.embargoed.fl_str_mv | 2021-10-09T04:55:36Z |
| datacite.rights.fl_str_mv | http://purl.org/coar/access_right/c_abf2 |
| datacite.subjects.subject.fl_str_mv | Cytotoxicity Glucose NADES Proline Thermal properties Urea Water mixtures Electronic, Optical and Magnetic Materials Atomic and Molecular Physics, and Optics Condensed Matter Physics Spectroscopy Physical and Theoretical Chemistry Materials Chemistry |
| datacite.titles.title.fl_str_mv | Influence of natural deep eutectic systems in water thermal behavior and their applications in cryopreservation |
| dc.contributor.none.fl_str_mv | LAQV@REQUIMTE DQ - Departamento de Química Elsevier RUN |
| dc.creator.none.fl_str_mv | Craveiro, Rita Castro, Vânia I. B. Viciosa, Maria Teresa Dionísio, Madalena Reis, R. L. Duarte, Ana Rita C. Paiva, Alexandre |
| dc.date.Accepted.fl_str_mv | 2021-05-01T00:00:00Z |
| dc.date.available.fl_str_mv | 2021-10-09T04:55:36Z |
| dc.date.embargoed.fl_str_mv | 2021-10-09T04:55:36Z |
| dc.format.none.fl_str_mv | application/pdf |
| dc.identifier.none.fl_str_mv | http://hdl.handle.net/10362/125817 |
| dc.language.none.fl_str_mv | eng |
| dc.rights.none.fl_str_mv | http://purl.org/coar/access_right/c_abf2 |
| dc.subject.none.fl_str_mv | Cytotoxicity Glucose NADES Proline Thermal properties Urea Water mixtures Electronic, Optical and Magnetic Materials Atomic and Molecular Physics, and Optics Condensed Matter Physics Spectroscopy Physical and Theoretical Chemistry Materials Chemistry |
| dc.title.fl_str_mv | Influence of natural deep eutectic systems in water thermal behavior and their applications in cryopreservation |
| dc.type.none.fl_str_mv | http://purl.org/coar/resource_type/c_6501 |
| description | Natural deep eutectic systems (NADES), which have applications as solvents for both engineering and life sciences, are mainly composed of sugars, aminoacids or organic acids. In this work NADES composed by glucose, urea and proline (G:U:P in a molar ratio of 1:1:1) and proline and glucose (P:G 5:3) were prepared and added in different mass fractions to water. By differential scanning calorimetry it was verified as the crystallization tendency of water is modified even for low fraction of NADES added. This is also observed by polarized optical microscopy which allowed following the formation of crystals with different crystalline morphologies as bulk water. Calorimetric data also shown as the crystallization temperature decreases for all P:G mixtures and this shift is more accentuated for weight fraction of NADES higher than 0.5. Crystallization is totally suppressed for NADES fraction higher than 0.7. NADES/water mixtures cytotoxicity was evaluated in vitro, revealing that they are less toxic as compared with the commonly used cryoprotective additives as dimethyl sulfoxide (DMSO). Additionally, cell viability tests with cell lines cryopreserved using DMSO and both the prepared NADES showed comparable viability. This work combines thermophysical data on NADES and evaluates it's in vitro performance, providing cues for their use in cryopreservation applications. |
| dirty | 0 |
| eu_rights_str_mv | openAccess |
| format | article |
| fulltext.url.fl_str_mv | https://run.unl.pt/bitstreams/d9387099-c805-4e14-831b-7506199d98d7/download |
| funder_facet_str_mv | FCT{{{_:::_}}}Fundação para a Ciência e a Tecnologia FCT{{{_:::_}}}Fundação para a Ciência e a Tecnologia FCT{{{_:::_}}}Fundação para a Ciência e a Tecnologia |
| funding.funder.alternateName_str_mv | FCT FCT FCT |
| funding.funder.identifier_str_mv | http://doi.org/10.13039/501100001871 http://doi.org/10.13039/501100001871 http://doi.org/10.13039/501100001871 |
| funding.funder.name_str_mv | Fundação para a Ciência e a Tecnologia Fundação para a Ciência e a Tecnologia Fundação para a Ciência e a Tecnologia |
| funding.identifier_str_mv | IF/01146/2015/CP1293/CT0003 UIDB/50006/2020 UIDP/50006/2020 |
| funding.name_str_mv | Investigador FCT 6817 - DCRRNI ID 6817 - DCRRNI ID |
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| identifier.url.fl_str_mv | http://hdl.handle.net/10362/125817 |
| inst_facet_str | urn:organizationAcronym:unl{{{_:::_}}}Universidade Nova de Lisboa |
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| institution | Universidade Nova de Lisboa |
| instname_str | Universidade Nova de Lisboa |
| language | eng |
| network_acronym_str | run |
| network_name_str | Repositório Institucional da UNL |
| oai_identifier_str | oai:run.unl.pt:10362/125817 |
| organization_str_mv | urn:organizationAcronym:unl |
| person_str_mv | Craveiro, Rita Castro, Vânia I. B. Viciosa, Maria Teresa Dionísio, Madalena Reis, R. L. Duarte, Ana Rita C. Paiva, Alexandre |
| publishDate | 2021 |
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| reponame_str | Repositório Institucional da UNL |
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| spelling | engenNatural deep eutectic systems (NADES), which have applications as solvents for both engineering and life sciences, are mainly composed of sugars, aminoacids or organic acids. In this work NADES composed by glucose, urea and proline (G:U:P in a molar ratio of 1:1:1) and proline and glucose (P:G 5:3) were prepared and added in different mass fractions to water. By differential scanning calorimetry it was verified as the crystallization tendency of water is modified even for low fraction of NADES added. This is also observed by polarized optical microscopy which allowed following the formation of crystals with different crystalline morphologies as bulk water. Calorimetric data also shown as the crystallization temperature decreases for all P:G mixtures and this shift is more accentuated for weight fraction of NADES higher than 0.5. Crystallization is totally suppressed for NADES fraction higher than 0.7. NADES/water mixtures cytotoxicity was evaluated in vitro, revealing that they are less toxic as compared with the commonly used cryoprotective additives as dimethyl sulfoxide (DMSO). Additionally, cell viability tests with cell lines cryopreserved using DMSO and both the prepared NADES showed comparable viability. This work combines thermophysical data on NADES and evaluates it's in vitro performance, providing cues for their use in cryopreservation applications.application/pdfenInfluence of natural deep eutectic systems in water thermal behavior and their applications in cryopreservationCraveiro, RitaCastro, Vânia I. B.Viciosa, Maria TeresaDionísio, MadalenaReis, R. L.Duarte, Ana Rita C.Paiva, AlexandreLAQV@REQUIMTEDQ - Departamento de QuímicaElsevierHostingInstitutionOrganizationalRUNe-mailmailto:run@unl.ptrun@unl.ptISSNIsPartOf0167-7322URNIsPartOfPURE: 28466706URNIsPartOfPURE UUID: d50a88f8-53ed-41e0-aab2-26113e72f6d7URNIsPartOfScopus: 85100660843URNIsPartOfWOS: 000636646200044URNIsPartOfORCID: /0000-0003-0800-0112/work/101182582URNIsPartOfORCID: /0000-0002-1487-0889/work/101182628DOIIsPartOf10.1016/j.molliq.2021.1155332021-10-09T04:55:36Z2021-05-012021-05-01T00:00:00ZHandlehttp://hdl.handle.net/10362/125817http://purl.org/coar/access_right/c_abf2open accessCytotoxicityGlucoseNADESProlineThermal propertiesUreaWater mixturesElectronic, Optical and Magnetic MaterialsAtomic and Molecular Physics, and OpticsCondensed Matter PhysicsSpectroscopyPhysical and Theoretical ChemistryMaterials Chemistry2432528 bytesFundação para a Ciência e a TecnologiaDES-ZYME: Biocatalysis in Natural Deep Eutectic Solvents.info:eu-repo/grantAgreement/FCT/Investigador FCT/IF%2F01146%2F2015%2FCP1293%2FCT0003/PTIF/01146/2015/CP1293/CT0003Investigador FCTCrossref Funder IDhttp://doi.org/10.13039/501100001871Fundação para a Ciência e a TecnologiaAssociated Laboratory for Green Chemistry - Clean Technologies and Processesinfo:eu-repo/grantAgreement/FCT/6817 - DCRRNI ID/UIDB%2F50006%2F2020/PTUIDB/50006/20206817 - DCRRNI IDCrossref Funder IDhttp://doi.org/10.13039/501100001871Fundação para a Ciência e a TecnologiaAssociated Laboratory for Green Chemistry - Clean Technologies and Processesinfo:eu-repo/grantAgreement/FCT/6817 - DCRRNI ID/UIDP%2F50006%2F2020/PTUIDP/50006/20206817 - DCRRNI IDCrossref Funder IDhttp://doi.org/10.13039/501100001871literaturehttp://purl.org/coar/resource_type/c_6501journal articlehttp://purl.org/coar/access_right/c_abf2application/pdffulltexthttps://run.unl.pt/bitstreams/d9387099-c805-4e14-831b-7506199d98d7/download |
| spellingShingle | Influence of natural deep eutectic systems in water thermal behavior and their applications in cryopreservation Craveiro, Rita Cytotoxicity Glucose NADES Proline Thermal properties Urea Water mixtures Electronic, Optical and Magnetic Materials Atomic and Molecular Physics, and Optics Condensed Matter Physics Spectroscopy Physical and Theoretical Chemistry Materials Chemistry |
| status | SINGLETON |
| subject.fl_str_mv | Cytotoxicity Glucose NADES Proline Thermal properties Urea Water mixtures Electronic, Optical and Magnetic Materials Atomic and Molecular Physics, and Optics Condensed Matter Physics Spectroscopy Physical and Theoretical Chemistry Materials Chemistry |
| title | Influence of natural deep eutectic systems in water thermal behavior and their applications in cryopreservation |
| title_full | Influence of natural deep eutectic systems in water thermal behavior and their applications in cryopreservation |
| title_fullStr | Influence of natural deep eutectic systems in water thermal behavior and their applications in cryopreservation |
| title_full_unstemmed | Influence of natural deep eutectic systems in water thermal behavior and their applications in cryopreservation |
| title_short | Influence of natural deep eutectic systems in water thermal behavior and their applications in cryopreservation |
| title_sort | Influence of natural deep eutectic systems in water thermal behavior and their applications in cryopreservation |
| topic | Cytotoxicity Glucose NADES Proline Thermal properties Urea Water mixtures Electronic, Optical and Magnetic Materials Atomic and Molecular Physics, and Optics Condensed Matter Physics Spectroscopy Physical and Theoretical Chemistry Materials Chemistry |
| topic_facet | Cytotoxicity Glucose NADES Proline Thermal properties Urea Water mixtures Electronic, Optical and Magnetic Materials Atomic and Molecular Physics, and Optics Condensed Matter Physics Spectroscopy Physical and Theoretical Chemistry Materials Chemistry |
| url | http://hdl.handle.net/10362/125817 |
| visible | 1 |