Publicação
Phenotypic and Molecular Traits of Staphylococcus coagulans Associated with Canine Skin Infections in Portugal
| Resumo: | Staphylococcus coagulans is among the three most frequent pathogens of canine pyoderma. Yet, studies on this species are scarce. Twenty-seven S. coagulans and one S. schleiferi, corresponding to all pyoderma-related isolations from these two species at two veterinary laboratories in Lisbon, Portugal, between 1999 and 2018 (Lab 1) or 2018 (Lab 2), were analyzed. Isolates were identified by the analysis of the nuc gene and urease production. Antibiotic susceptibility towards 27 antibiotics was evaluated by disk diffusion. Fourteen antibiotic resistance genes were screened by PCR. Isolates were typed by SmaI-PFGE. Two S. coagulans isolates (2/27, 7.4%) were methicillin-resistant (MRSC, mecA+) and four (4/27, 14.8%) displayed a multidrug-resistant (MDR) phenotype. We observed resistance to penicillin (17/27, 63.0%), fluoroquinolones (11/27, 40.7%), erythromycin and clindamycin (3/27, 11.1%), fusidic acid (3/27, 11.1%) and tetracycline (1/27, 3.7%). The blaZ and erm(B) genes were carried by 16 and 1 isolates resistant to penicillin and erythromycin/clindamycin, respectively. Only three S. coagulans carried plasmids. The single S. schleiferi isolate presented an MDR phenotype. SmaI-PFGE revealed a limited genetic diversity of S. coagulans, with a predominant lineage present from 2001 to 2018. This study describes the first MRSC causing canine infection in Portugal and reveals a high burden of antimicrobial resistance, with the emergence of MDR phenotypes within the main lineages. |
|---|---|
| Autores principais: | Costa, Sofia Santos |
| Outros Autores: | Oliveira, Valéria; Serrano, Maria; Pomba, Constança; Couto, Isabel |
| Assunto: | Microbiology Infectious Diseases General Veterinary Molecular Biology SDG 3 - Good Health and Well-being SDG 12 - Responsible Consumption and Production SDG 15 - Life on Land |
| Ano: | 2021 |
| País: | Portugal |
| Tipo de documento: | artigo |
| Tipo de acesso: | acesso aberto |
| Instituição associada: | Universidade Nova de Lisboa |
| Idioma: | inglês |
| Origem: | Repositório Institucional da UNL |
| _version_ | 1879447933919690752 |
|---|---|
| author | Costa, Sofia Santos |
| author2 | Oliveira, Valéria Serrano, Maria Pomba, Constança Couto, Isabel |
| author2_role | author author author author |
| author_facet | Costa, Sofia Santos Oliveira, Valéria Serrano, Maria Pomba, Constança Couto, Isabel |
| author_role | author |
| contributor_name_str_mv | Instituto de Higiene e Medicina Tropical (IHMT) Global Health and Tropical Medicine (GHTM) TB, HIV and opportunistic diseases and pathogens (THOP) MDPI - Multidisciplinary Digital Publishing Institute RUN |
| country_str | PT |
| creators_json_txt | [{\"Person.name\":\"Costa, Sofia Santos\"},{\"Person.name\":\"Oliveira, Valéria\"},{\"Person.name\":\"Serrano, Maria\"},{\"Person.name\":\"Pomba, Constança\"},{\"Person.name\":\"Couto, Isabel\"}] |
| datacite.contributors.contributor.contributorName.fl_str_mv | Instituto de Higiene e Medicina Tropical (IHMT) Global Health and Tropical Medicine (GHTM) TB, HIV and opportunistic diseases and pathogens (THOP) MDPI - Multidisciplinary Digital Publishing Institute RUN |
| datacite.creators.creator.creatorName.fl_str_mv | Costa, Sofia Santos Oliveira, Valéria Serrano, Maria Pomba, Constança Couto, Isabel |
| datacite.date.Accepted.fl_str_mv | 2021-05-02T00:00:00Z |
| datacite.date.available.fl_str_mv | 2021-06-14T22:16:14Z |
| datacite.date.embargoed.fl_str_mv | 2021-06-14T22:16:14Z |
| datacite.rights.fl_str_mv | http://purl.org/coar/access_right/c_abf2 |
| datacite.subjects.subject.fl_str_mv | Microbiology Infectious Diseases General Veterinary Molecular Biology SDG 3 - Good Health and Well-being SDG 12 - Responsible Consumption and Production SDG 15 - Life on Land |
| datacite.titles.title.fl_str_mv | Phenotypic and Molecular Traits of Staphylococcus coagulans Associated with Canine Skin Infections in Portugal |
| dc.contributor.none.fl_str_mv | Instituto de Higiene e Medicina Tropical (IHMT) Global Health and Tropical Medicine (GHTM) TB, HIV and opportunistic diseases and pathogens (THOP) MDPI - Multidisciplinary Digital Publishing Institute RUN |
| dc.creator.none.fl_str_mv | Costa, Sofia Santos Oliveira, Valéria Serrano, Maria Pomba, Constança Couto, Isabel |
| dc.date.Accepted.fl_str_mv | 2021-05-02T00:00:00Z |
| dc.date.available.fl_str_mv | 2021-06-14T22:16:14Z |
| dc.date.embargoed.fl_str_mv | 2021-06-14T22:16:14Z |
| dc.format.none.fl_str_mv | application/pdf |
| dc.identifier.none.fl_str_mv | http://hdl.handle.net/10362/119234 |
| dc.language.none.fl_str_mv | eng |
| dc.rights.none.fl_str_mv | http://purl.org/coar/access_right/c_abf2 |
| dc.subject.none.fl_str_mv | Microbiology Infectious Diseases General Veterinary Molecular Biology SDG 3 - Good Health and Well-being SDG 12 - Responsible Consumption and Production SDG 15 - Life on Land |
| dc.title.fl_str_mv | Phenotypic and Molecular Traits of Staphylococcus coagulans Associated with Canine Skin Infections in Portugal |
| dc.type.none.fl_str_mv | http://purl.org/coar/resource_type/c_6501 |
| description | Staphylococcus coagulans is among the three most frequent pathogens of canine pyoderma. Yet, studies on this species are scarce. Twenty-seven S. coagulans and one S. schleiferi, corresponding to all pyoderma-related isolations from these two species at two veterinary laboratories in Lisbon, Portugal, between 1999 and 2018 (Lab 1) or 2018 (Lab 2), were analyzed. Isolates were identified by the analysis of the nuc gene and urease production. Antibiotic susceptibility towards 27 antibiotics was evaluated by disk diffusion. Fourteen antibiotic resistance genes were screened by PCR. Isolates were typed by SmaI-PFGE. Two S. coagulans isolates (2/27, 7.4%) were methicillin-resistant (MRSC, mecA+) and four (4/27, 14.8%) displayed a multidrug-resistant (MDR) phenotype. We observed resistance to penicillin (17/27, 63.0%), fluoroquinolones (11/27, 40.7%), erythromycin and clindamycin (3/27, 11.1%), fusidic acid (3/27, 11.1%) and tetracycline (1/27, 3.7%). The blaZ and erm(B) genes were carried by 16 and 1 isolates resistant to penicillin and erythromycin/clindamycin, respectively. Only three S. coagulans carried plasmids. The single S. schleiferi isolate presented an MDR phenotype. SmaI-PFGE revealed a limited genetic diversity of S. coagulans, with a predominant lineage present from 2001 to 2018. This study describes the first MRSC causing canine infection in Portugal and reveals a high burden of antimicrobial resistance, with the emergence of MDR phenotypes within the main lineages. |
| dirty | 0 |
| eu_rights_str_mv | openAccess |
| format | article |
| fulltext.url.fl_str_mv | https://run.unl.pt/bitstreams/5af8a41c-9ddd-4167-87bc-04ba0f9e13be/download |
| id | run_ae94c69f458e7969cc5fab65038f93db |
| identifier.url.fl_str_mv | http://hdl.handle.net/10362/119234 |
| inst_facet_str | urn:organizationAcronym:unl{{{_:::_}}}Universidade Nova de Lisboa |
| instacron_str | unl |
| institution | Universidade Nova de Lisboa |
| instname_str | Universidade Nova de Lisboa |
| language | eng |
| network_acronym_str | run |
| network_name_str | Repositório Institucional da UNL |
| oai_identifier_str | oai:run.unl.pt:10362/119234 |
| organization_str_mv | urn:organizationAcronym:unl |
| person_str_mv | Costa, Sofia Santos Oliveira, Valéria Serrano, Maria Pomba, Constança Couto, Isabel |
| publishDate | 2021 |
| repo_facet_str | urn:repositoryAcronym:run{{{_:::_}}}Repositório Institucional da UNL |
| reponame_str | Repositório Institucional da UNL |
| repository_id_str | urn:repositoryAcronym:run |
| service_str_mv | urn:repositoryAcronym:run |
| spelling | engenStaphylococcus coagulans is among the three most frequent pathogens of canine pyoderma. Yet, studies on this species are scarce. Twenty-seven S. coagulans and one S. schleiferi, corresponding to all pyoderma-related isolations from these two species at two veterinary laboratories in Lisbon, Portugal, between 1999 and 2018 (Lab 1) or 2018 (Lab 2), were analyzed. Isolates were identified by the analysis of the nuc gene and urease production. Antibiotic susceptibility towards 27 antibiotics was evaluated by disk diffusion. Fourteen antibiotic resistance genes were screened by PCR. Isolates were typed by SmaI-PFGE. Two S. coagulans isolates (2/27, 7.4%) were methicillin-resistant (MRSC, mecA+) and four (4/27, 14.8%) displayed a multidrug-resistant (MDR) phenotype. We observed resistance to penicillin (17/27, 63.0%), fluoroquinolones (11/27, 40.7%), erythromycin and clindamycin (3/27, 11.1%), fusidic acid (3/27, 11.1%) and tetracycline (1/27, 3.7%). The blaZ and erm(B) genes were carried by 16 and 1 isolates resistant to penicillin and erythromycin/clindamycin, respectively. Only three S. coagulans carried plasmids. The single S. schleiferi isolate presented an MDR phenotype. SmaI-PFGE revealed a limited genetic diversity of S. coagulans, with a predominant lineage present from 2001 to 2018. This study describes the first MRSC causing canine infection in Portugal and reveals a high burden of antimicrobial resistance, with the emergence of MDR phenotypes within the main lineages.application/pdfenPhenotypic and Molecular Traits of Staphylococcus coagulans Associated with Canine Skin Infections in PortugalCosta, Sofia SantosOliveira, ValériaSerrano, MariaPomba, ConstançaCouto, IsabelInstituto de Higiene e Medicina Tropical (IHMT)Global Health and Tropical Medicine (GHTM)TB, HIV and opportunistic diseases and pathogens (THOP)MDPI - Multidisciplinary Digital Publishing InstituteHostingInstitutionOrganizationalRUNe-mailmailto:run@unl.ptrun@unl.ptISSNIsPartOf2079-6382URNIsPartOfPURE: 31529875URNIsPartOfPURE UUID: 629018f8-754a-4546-869b-a874491c5a66URNIsPartOfORCID: /0000-0002-4096-2410/work/93163098URNIsPartOfScopus: 85105922622URNIsPartOfORCID: /0000-0003-3536-2733/work/94752369DOIIsPartOf10.3390/antibiotics100505182021-06-14T22:16:14Z2021-05-022021-05-02T00:00:00ZHandlehttp://hdl.handle.net/10362/119234http://purl.org/coar/access_right/c_abf2open accessMicrobiologyInfectious DiseasesGeneral VeterinaryMolecular BiologySDG 3 - Good Health and Well-beingSDG 12 - Responsible Consumption and ProductionSDG 15 - Life on Land1245089 bytesliteraturehttp://purl.org/coar/resource_type/c_6501journal articlehttp://purl.org/coar/access_right/c_abf2application/pdffulltexthttps://run.unl.pt/bitstreams/5af8a41c-9ddd-4167-87bc-04ba0f9e13be/download |
| spellingShingle | Phenotypic and Molecular Traits of Staphylococcus coagulans Associated with Canine Skin Infections in Portugal Costa, Sofia Santos Microbiology Infectious Diseases General Veterinary Molecular Biology SDG 3 - Good Health and Well-being SDG 12 - Responsible Consumption and Production SDG 15 - Life on Land |
| status | SINGLETON |
| subject.fl_str_mv | Microbiology Infectious Diseases General Veterinary Molecular Biology SDG 3 - Good Health and Well-being SDG 12 - Responsible Consumption and Production SDG 15 - Life on Land |
| title | Phenotypic and Molecular Traits of Staphylococcus coagulans Associated with Canine Skin Infections in Portugal |
| title_full | Phenotypic and Molecular Traits of Staphylococcus coagulans Associated with Canine Skin Infections in Portugal |
| title_fullStr | Phenotypic and Molecular Traits of Staphylococcus coagulans Associated with Canine Skin Infections in Portugal |
| title_full_unstemmed | Phenotypic and Molecular Traits of Staphylococcus coagulans Associated with Canine Skin Infections in Portugal |
| title_short | Phenotypic and Molecular Traits of Staphylococcus coagulans Associated with Canine Skin Infections in Portugal |
| title_sort | Phenotypic and Molecular Traits of Staphylococcus coagulans Associated with Canine Skin Infections in Portugal |
| topic | Microbiology Infectious Diseases General Veterinary Molecular Biology SDG 3 - Good Health and Well-being SDG 12 - Responsible Consumption and Production SDG 15 - Life on Land |
| topic_facet | Microbiology Infectious Diseases General Veterinary Molecular Biology SDG 3 - Good Health and Well-being SDG 12 - Responsible Consumption and Production SDG 15 - Life on Land |
| url | http://hdl.handle.net/10362/119234 |
| visible | 1 |
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