Publication
Low-Phytotoxic Deep Eutectic Systems as Alternative Extraction Media for the Recovery of Chitin from Brown Crab Shells
| Summary: | The versatility of chitin and its derivatives has allowed their utilization in a wide range of applications, from wastewater treatment to pharmaceutical or biomedical industries. However, even though the extraction method used industrially is extremely efficient, it involves the use of strong acids and bases and results in the disposal of large quantities of toxic effluents. Deep eutectic systems (DESs) have emerged as a promising new class of alternative solvents, including for chitin recovery. Yet, the assessment of their toxicity has often been neglected. Therefore, in this work, the phytotoxicity of choline chloride (ChCl)/organic acid-based DESs toward wheat seeds was evaluated by measuring different growth parameters and stress biomarkers. DESs were then explored for the efficient recovery of chitin contained in brown crab shell residues at varying conditions of temperature and processing time as well as with and without water addition. The obtained chitin was then characterized through different analytical techniques and compared to a standard as well as to chitin obtained by a conventional acid/alkaline hydrolysis. Results have shown that by applying a ChCl/lactic acid-based DES (which was the system that showed the least phytotoxic effects on wheat; EC50 ≥ 1.6 mg/mL) at 130 °C, it was possible to obtain pure chitin (up to 98%) with characteristics similar to those presented by commercial chitin or chitin recovered by conventional hydrolysis in a shorter time (more than 8-fold faster), thus suggesting that ChCl/organic acid-based DESs can truly represent a low-phytotoxic alternative extraction media for the recovery of chitin from the crab shell biomass. |
|---|---|
| Main Authors: | Rodrigues, Liliana A. |
| Other Authors: | Radojčić Redovniković, Ivana; Duarte, Ana Rita C.; Matias, Ana A.; Paiva, Alexandre |
| Subject: | General Chemistry General Chemical Engineering |
| Year: | 2021 |
| Country: | Portugal |
| Document type: | article |
| Access type: | open access |
| Associated institution: | Universidade Nova de Lisboa |
| Language: | English |
| Origin: | Repositório Institucional da UNL |
| _version_ | 1879447297965686784 |
|---|---|
| author | Rodrigues, Liliana A. |
| author2 | Radojčić Redovniković, Ivana Duarte, Ana Rita C. Matias, Ana A. Paiva, Alexandre |
| author2_role | author author author author |
| author_facet | Rodrigues, Liliana A. Radojčić Redovniković, Ivana Duarte, Ana Rita C. Matias, Ana A. Paiva, Alexandre |
| author_role | author |
| contributor_name_str_mv | Instituto de Tecnologia Química e Biológica António Xavier (ITQB) DQ - Departamento de Química LAQV@REQUIMTE ACS - American Chemical Society RUN |
| country_str | PT |
| creators_json_txt | [{\"Person.name\":\"Rodrigues, Liliana A.\"},{\"Person.name\":\"Radojčić Redovniković, Ivana\"},{\"Person.name\":\"Duarte, Ana Rita C.\"},{\"Person.name\":\"Matias, Ana A.\"},{\"Person.name\":\"Paiva, Alexandre\"}] |
| datacite.contributors.contributor.contributorName.fl_str_mv | Instituto de Tecnologia Química e Biológica António Xavier (ITQB) DQ - Departamento de Química LAQV@REQUIMTE ACS - American Chemical Society RUN |
| datacite.creators.creator.creatorName.fl_str_mv | Rodrigues, Liliana A. Radojčić Redovniković, Ivana Duarte, Ana Rita C. Matias, Ana A. Paiva, Alexandre |
| datacite.date.Accepted.fl_str_mv | 2021-11-02T00:00:00Z |
| datacite.date.available.fl_str_mv | 2021-12-20T23:17:49Z |
| datacite.date.embargoed.fl_str_mv | 2021-12-20T23:17:49Z |
| datacite.rights.fl_str_mv | http://purl.org/coar/access_right/c_abf2 |
| datacite.subjects.subject.fl_str_mv | General Chemistry General Chemical Engineering |
| datacite.titles.title.fl_str_mv | Low-Phytotoxic Deep Eutectic Systems as Alternative Extraction Media for the Recovery of Chitin from Brown Crab Shells |
| dc.contributor.none.fl_str_mv | Instituto de Tecnologia Química e Biológica António Xavier (ITQB) DQ - Departamento de Química LAQV@REQUIMTE ACS - American Chemical Society RUN |
| dc.creator.none.fl_str_mv | Rodrigues, Liliana A. Radojčić Redovniković, Ivana Duarte, Ana Rita C. Matias, Ana A. Paiva, Alexandre |
| dc.date.Accepted.fl_str_mv | 2021-11-02T00:00:00Z |
| dc.date.available.fl_str_mv | 2021-12-20T23:17:49Z |
| dc.date.embargoed.fl_str_mv | 2021-12-20T23:17:49Z |
| dc.format.none.fl_str_mv | application/pdf |
| dc.identifier.none.fl_str_mv | http://hdl.handle.net/10362/129530 |
| dc.language.none.fl_str_mv | eng |
| dc.rights.none.fl_str_mv | http://purl.org/coar/access_right/c_abf2 |
| dc.subject.none.fl_str_mv | General Chemistry General Chemical Engineering |
| dc.title.fl_str_mv | Low-Phytotoxic Deep Eutectic Systems as Alternative Extraction Media for the Recovery of Chitin from Brown Crab Shells |
| dc.type.none.fl_str_mv | http://purl.org/coar/resource_type/c_6501 |
| description | The versatility of chitin and its derivatives has allowed their utilization in a wide range of applications, from wastewater treatment to pharmaceutical or biomedical industries. However, even though the extraction method used industrially is extremely efficient, it involves the use of strong acids and bases and results in the disposal of large quantities of toxic effluents. Deep eutectic systems (DESs) have emerged as a promising new class of alternative solvents, including for chitin recovery. Yet, the assessment of their toxicity has often been neglected. Therefore, in this work, the phytotoxicity of choline chloride (ChCl)/organic acid-based DESs toward wheat seeds was evaluated by measuring different growth parameters and stress biomarkers. DESs were then explored for the efficient recovery of chitin contained in brown crab shell residues at varying conditions of temperature and processing time as well as with and without water addition. The obtained chitin was then characterized through different analytical techniques and compared to a standard as well as to chitin obtained by a conventional acid/alkaline hydrolysis. Results have shown that by applying a ChCl/lactic acid-based DES (which was the system that showed the least phytotoxic effects on wheat; EC50 ≥ 1.6 mg/mL) at 130 °C, it was possible to obtain pure chitin (up to 98%) with characteristics similar to those presented by commercial chitin or chitin recovered by conventional hydrolysis in a shorter time (more than 8-fold faster), thus suggesting that ChCl/organic acid-based DESs can truly represent a low-phytotoxic alternative extraction media for the recovery of chitin from the crab shell biomass. |
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| person_str_mv | Rodrigues, Liliana A. Radojčić Redovniković, Ivana Duarte, Ana Rita C. Matias, Ana A. Paiva, Alexandre |
| publishDate | 2021 |
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| spelling | engenThe versatility of chitin and its derivatives has allowed their utilization in a wide range of applications, from wastewater treatment to pharmaceutical or biomedical industries. However, even though the extraction method used industrially is extremely efficient, it involves the use of strong acids and bases and results in the disposal of large quantities of toxic effluents. Deep eutectic systems (DESs) have emerged as a promising new class of alternative solvents, including for chitin recovery. Yet, the assessment of their toxicity has often been neglected. Therefore, in this work, the phytotoxicity of choline chloride (ChCl)/organic acid-based DESs toward wheat seeds was evaluated by measuring different growth parameters and stress biomarkers. DESs were then explored for the efficient recovery of chitin contained in brown crab shell residues at varying conditions of temperature and processing time as well as with and without water addition. The obtained chitin was then characterized through different analytical techniques and compared to a standard as well as to chitin obtained by a conventional acid/alkaline hydrolysis. Results have shown that by applying a ChCl/lactic acid-based DES (which was the system that showed the least phytotoxic effects on wheat; EC50 ≥ 1.6 mg/mL) at 130 °C, it was possible to obtain pure chitin (up to 98%) with characteristics similar to those presented by commercial chitin or chitin recovered by conventional hydrolysis in a shorter time (more than 8-fold faster), thus suggesting that ChCl/organic acid-based DESs can truly represent a low-phytotoxic alternative extraction media for the recovery of chitin from the crab shell biomass.application/pdfenLow-Phytotoxic Deep Eutectic Systems as Alternative Extraction Media for the Recovery of Chitin from Brown Crab ShellsRodrigues, Liliana A.Radojčić Redovniković, IvanaDuarte, Ana Rita C.Matias, Ana A.Paiva, AlexandreInstituto de Tecnologia Química e Biológica António Xavier (ITQB)DQ - Departamento de QuímicaLAQV@REQUIMTEACS - American Chemical SocietyHostingInstitutionOrganizationalRUNe-mailmailto:run@unl.ptrun@unl.ptISSNIsPartOf2470-1343URNIsPartOfPURE: 35417650URNIsPartOfPURE UUID: b46f1c8d-0e65-4d70-9404-f6e644246e5cURNIsPartOfScopus: 85118599160URNIsPartOfPubMed: 34746567URNIsPartOfPubMedCentral: PMC8567356URNIsPartOfWOS: 000714105800028URNIsPartOfORCID: /0000-0003-0800-0112/work/105094938DOIIsPartOf10.1021/acsomega.1c034022021-12-20T23:17:49Z2021-11-022021-11-02T00:00:00ZHandlehttp://hdl.handle.net/10362/129530http://purl.org/coar/access_right/c_abf2open accessGeneral ChemistryGeneral Chemical Engineering5989775 bytesFundação para a Ciência e a TecnologiaiNOVA4Health - Programme in Translational Medicine (iBET, CEDOC/FCM, IPOLFG e ITQB)info:eu-repo/grantAgreement/FCT/6817 - DCRRNI ID/UIDB%2F04462%2F2020/PTUIDB/04462/20206817 - DCRRNI IDCrossref Funder IDhttp://doi.org/10.13039/501100001871Fundação para a Ciência e a TecnologiaAssociated Laboratory for Green Chemistry - Clean Technologies and Processesinfo:eu-repo/grantAgreement/FCT/6817 - DCRRNI ID/UIDB%2F50006%2F2020/PTUIDB/50006/20206817 - DCRRNI IDCrossref Funder IDhttp://doi.org/10.13039/501100001871Fundação para a Ciência e a TecnologiaDevelopment of process technologies for the valorization of fish processing waste streams: integration of natural deep eutectic solvents NADES with supercritical CO2 extractioninfo:eu-repo/grantAgreement/FCT/OE/SFRH%2FBD%2F116002%2F2016/PTSFRH/BD/116002/2016OECrossref Funder IDhttp://doi.org/10.13039/501100001871Fundação para a Ciência e a TecnologiaDES-ZYME: Biocatalysis in Natural Deep Eutectic Solvents.info:eu-repo/grantAgreement/FCT/Investigador FCT/IF%2F01146%2F2015%2FCP1293%2FCT0003/PTIF/01146/2015/CP1293/CT0003Investigador FCTCrossref Funder IDhttp://doi.org/10.13039/501100001871Fundação para a Ciência e a TecnologiaGRAPHYT - Towards a biorefinery approach of agricultural wastes and agri-food co-products streams: GReen solid-liquid Adsorption processes for PHYTochemicals isolationinfo:eu-repo/grantAgreement/FCT/Investigador FCT/IF%2F00723%2F2014%2FCP1229%2FCT0002/PTIF/00723/2014/CP1229/CT0002Investigador FCTCrossref Funder IDhttp://doi.org/10.13039/501100001871literaturehttp://purl.org/coar/resource_type/c_6501journal articlehttp://purl.org/coar/access_right/c_abf2application/pdffulltexthttps://run.unl.pt/bitstreams/9a08511c-0cb8-47b8-8982-50f58fed5e33/download |
| spellingShingle | Low-Phytotoxic Deep Eutectic Systems as Alternative Extraction Media for the Recovery of Chitin from Brown Crab Shells Rodrigues, Liliana A. General Chemistry General Chemical Engineering |
| status | SINGLETON |
| subject.fl_str_mv | General Chemistry General Chemical Engineering |
| title | Low-Phytotoxic Deep Eutectic Systems as Alternative Extraction Media for the Recovery of Chitin from Brown Crab Shells |
| title_full | Low-Phytotoxic Deep Eutectic Systems as Alternative Extraction Media for the Recovery of Chitin from Brown Crab Shells |
| title_fullStr | Low-Phytotoxic Deep Eutectic Systems as Alternative Extraction Media for the Recovery of Chitin from Brown Crab Shells |
| title_full_unstemmed | Low-Phytotoxic Deep Eutectic Systems as Alternative Extraction Media for the Recovery of Chitin from Brown Crab Shells |
| title_short | Low-Phytotoxic Deep Eutectic Systems as Alternative Extraction Media for the Recovery of Chitin from Brown Crab Shells |
| title_sort | Low-Phytotoxic Deep Eutectic Systems as Alternative Extraction Media for the Recovery of Chitin from Brown Crab Shells |
| topic | General Chemistry General Chemical Engineering |
| topic_facet | General Chemistry General Chemical Engineering |
| url | http://hdl.handle.net/10362/129530 |
| visible | 1 |